Antibiotic Sensitivity and Histopathological Study of Clostridium perfringens Associated with Necrotic Enteritis in Poultry

Shelke, Prerana; Pawade, M.M.; Mhase, P. P.; Mehere, Prajwalini; Sangle, Jyotika D.

doi:10.20546/ijcmas.2018.711.363

Cited by 3 publications

(6 citation statements)

References 9 publications

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“…However, a lower level of susceptibility was detected to CHL (36.0%). These findings are partially corroborated by other studies [ 26 , 45 - 47 ]. Alarmingly, a few antimicrobials such as ERY, gentamycin, and OTE were captured as S to 0.0% of isolates.…”

Section: Discussionsupporting

confidence: 90%

“…The samples were processed immediately after arrival at the designated laboratory as per the standard method [ 25 ]. Isolation and identification of C. perfringens were accomplished as per the protocol described earlier [ 26 ]. In brief, initial enrichment was made using the processed sample in Robertson’s Cooked Meat media (RCM broth), (HiMedia, Mumbai, India), subsequently, anaerobic incubation at 37°C for 24 h through candle jar method.…”

Section: Methodsmentioning

confidence: 99%

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Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

et al. 2021

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Background and Aim: Necrotic enteritis (NE) is one of the most prevalent diseases in broiler poultry caused by Clostridium perfringens connected with significant economic losses. A cross-sectional survey was conducted in Mymensingh district of Bangladesh to assess the prevalence of C. perfringens through toxinotyping molecular assay and confirm the risk factors for NE, including antimicrobial-resistant (AMR) status of the isolates. Materials and Methods: We included 40 small-scale commercial broiler farms randomly selected from two subdistricts of Mymensingh district of Bangladesh. As an individual sample, 240 cloacal swabs, and as a pooled sample, 40 drinking water, 40 workers' hand washing, 40 litter swab, and 40 feed samples were collected and evaluated by culture, biochemical, and molecular assays. A pretested semi-structured interview questionnaire was employed to capture flock-level data on risk factors from the farm owners. The flock-level data on risk factors were assessed through univariable and multivariable logistic regression analyses with p<0.05 was considered statistically significant. Results: Overall flock-level prevalence of C. perfringens was estimated to be 10.3% (95% confidence interval [CI] 7.5- 13.6%). Litter swab (pooled) was found to be highly contaminated with C. perfringens (25.0%, 95% CI: 12.7-41.2%) followed by the cloacal swab (10.4%, 95% CI: 6.9-15.0%) and feed sample (5.0%, 95% CI: 0.6-16.9%). History of coccidia infection (Adjusted odds ratio =33.01, 95% CI: 2.14-507.59, p=0.01) was significantly associated with flock-level C. perfringens infection status. In this study, 78.1% isolates were found as multidrug-resistant as they demonstrated resistance to 3-5 antimicrobial agents. Conclusion: Evidence-based control options need to be taken through the uses of prebiotics and probiotics, biosecurity, and hygienic measurement, including control of coccidia infection, is needed to lessen the NE infection and AMR related to this pathogen in small-scale commercial broiler poultry.

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Section: Discussionsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

et al. 2021

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“…ii. Staining properties and microscopic observation: The smear was prepared from suspected bacterial colonies followed by Gram-staining method and examined under a light microscope to observe the nature, shape, size, and other criteria of Clostridium species as per standard protocol (Shelke et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

“…It plays a major role in necrotic enteritis (food poisoning) in humans and animals (Xiu et al, 2020). In humans, the clinical signs are vomiting, abdominal pain, abdominal cramps, tenesmus, and bloody diarrhoea beginning within 24 hours and lasting less than 24 hours (Nyrah et al, 2017;Shelke et al, 2018: Samul et al, 2013.…”

Section: Introductionmentioning

confidence: 99%

Toxin-producing Clostridium perfringens in cooked cereal food in restaurants in Bangladesh

MA,

et al. 2024

Bangl. vet.

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Clostridium perfringens causes food poisoning in humans worldwide and C. perfringens beta toxin is associated with improperly heated or reheated cooked food. A study was undertaken to determine the prevalence of beta toxin-producing C. perfringens in cooked Plain rice, Pulao, and Biryani in different types of restaurants (general, well furnished) of four districts [Dhaka (north and south city corporation area), Cumilla, Narayangonj, Gazipur)] of Bangladesh. A total of 200 food samples were examined for the presence of C. perfringens and its beta toxin. The positive samples were further tested for the CPB gene (236 bp) of beta toxin-producing C. perfringens using PCR assay. Three samples had C. perfringens (1.5%), in food samples of the restaurants in Dhaka South City Corporation (DSCC). Beta toxin-producing C. perfringens was in one sample (0.5%) in a sample of pulao in the same area during the winter. It is suggested that the prevalence of beta toxin-producing C. perfringens was low but, further studies are required in other cities in Bangladesh. Bangl. vet. 2023. Vol. 40, No. 1-2, 8–15

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“…Standard methods were employed for processing samples immediately after arrival at the laboratory (Ezatkhah et al, 2016). An earlier described protocol was used for the isolation and identification of C. perfringens (Shelke et al, 2018). In this method, Robertson's Cooked meat media (RCM broth), (HiMedia, Mumbai, India) was used for initial enrichment of the processed sample, and afterward, anaerobic incubation was done at 37°C for 24 h via candle jar method.…”

Section: Detection Of C Perfringens 241 Culture and Biochemical Assaysmentioning

confidence: 99%

Occurrence of Clostridium perfringens in layer flocks of selected districts in Bangladesh: molecular typing, antimicrobial susceptibility

Arif

Sultana

Islam

et al. 2022

Asian Australas. J. Biosci. Biotechnol.

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Necrotic enteritis (NE) is one of the important enteric diseases in poultry caused by Clostridium perfringens which leads to considerable economic losses for poultry raisers. The present research was carried out to estimate prevalence, risk factors and to confirm molecular pattern, antimicrobial susceptibility status of Clostridium perfringens in layer flocks in Mymensingh and Gazipur districts of Bangladesh, where a total of 287 samples were collected that comprised of water (30), workers’ hand washing (30), and feed (30) as environmental pooled samples; cloacal swab (150) and post mortem sample (intestine) (47) as individual samples, and evaluated by basic (culture, biochemical test), and finally toxinotyping multiplex PCR assays. The flock-level data on potential risk factors were collected through semi-structured interviews with the farm owners and evaluated via univariable and multivariable logistic regression analyses where a p-value of <0.05 was considered as statistically significant. Overall flock-level prevalence of Clostridium perfringens was confirmed to be 6.3% (95% confidence interval [CI]: 3.8-9.7%). The occurrence of C. perfringens was found to be higher in the intestine samples (12.8%, 95% CI: 4.8- 25.7%) followed by feed (6.7%, 95% CI: 0.8-22.1%), cloacal swabs (6.0%, 95% CI: 2.8-11.1%) and workers’ hand washing (3.3%, 95% CI: 0.1- 12.2%). History of immune-suppressive disease (like New castle disease/egg drop syndrome/infectious bursal disease) [Odds Ratio (OR) = 22.16, 95% CI: 1.40-349.36, P = 0.027] and flock size more than 3000 birds (OR = 11.16, 95% CI: 1.26-98.25, P = 0.029) were found to be associated with C. perfringens infection status in the layer flocks. In this study, C. perfringens toxinotype A is circulating in layer flocks with 72.2% of isolates were established as multidrug-resistant as they demonstrated resistance between 3 to 6 antimicrobial agents. Evidence-based control measures to be taken namely use of probiotics, prebiotic, organic acids, and plant extracts as natural alternatives including control of immune-suppressive diseases are needed to minimize the NE infection and antimicrobial resistance in low biosecurity layer flocks. Asian Australas. J. Biosci. Biotechnol. 2022, 7(2), 36-49

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Antibiotic Sensitivity and Histopathological Study of Clostridium perfringens Associated with Necrotic Enteritis in Poultry

Cited by 3 publications

References 9 publications

Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

Toxin-producing Clostridium perfringens in cooked cereal food in restaurants in Bangladesh

Occurrence of Clostridium perfringens in layer flocks of selected districts in Bangladesh: molecular typing, antimicrobial susceptibility

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