Antibodies to structural and nonstructural gag-coded proteins of type D retroviruses in patients with lymphadenopathies

Morozov, V A; Il'inskii, P. O.; Ильин, К. В.

doi:10.1007/bf00802184

Cited by 5 publications

(6 citation statements)

References 6 publications

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“…Most of these, however, were carried out in groups of patients with specific diagnoses (8,19,25) or in populations with no specific exposure to NHPs (4,26,27). For the majority of these studies, the results have been inconclusive, describing only partial seroreactivity, most commonly to a single SRV gag gene product (e.g., p25 or p27) by Western immunoblotting (WB) (4,8,19,(25)(26)(27). Type D retroviruses have also been isolated from human cell lines in culture, but the majority of these infections have been attributed to laboratory contamination (13,28).…”

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confidence: 99%

Evidence of Infection with Simian Type D Retrovirus in Persons Occupationally Exposed to Nonhuman Primates

Lerche

Switzer

Yee

et al. 2001

J Virol

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Simian type D retrovirus (SRV) is enzootic in many populations of Asian monkeys of the genusMacaca and is associated with immunodeficiency diseases. However, the zoonotic potential of this agent has not been well defined. Screening for antibodies to SRV was performed as part of an ongoing study looking for evidence of infection with simian retroviruses among persons occupationally exposed to nonhuman primates (NHPs). Of 231 persons tested, 2 (0.9%) were found to be strongly seropositive, showing reactivity against multiple SRV antigens representing gag, pol, and env gene products by Western immunoblotting. Persistent long-standing seropositivity, as well as neutralizing antibody specific to SRV type 2, was documented in one individual (subject 1), while waning antibody with eventual seroreversion was observed in a second (subject 2). Repeated attempts to detect SRV by isolation in tissue culture and by using sensitive PCR assays for amplification of two SRV gene regions (gag and pol) were negative. Both individuals remain apparently healthy. We were also unable to transmit this seropositivity to an SRV-negative macaque by using inoculation of whole blood from subject 1. The results of this study provide evidence that occupational exposure to NHPs may increase the risk of infection with SRV and underscore the importance of both occupational safety practices and efforts to eliminate this virus from established macaque colonies.

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confidence: 99%

Evidence of Infection with Simian Type D Retrovirus in Persons Occupationally Exposed to Nonhuman Primates

Lerche

Switzer

Yee

et al. 2001

J Virol

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“…A number of established human cell lines were found to harbor MPMV or other type D viruses (11,18,24,25,30,36,37,46,47), but these infections were usually ascribed to laboratory contaminations (30, 47). Other investigators have described MPMVreactive antisera from normal humans (22,35,45) and those with AIDS-related or non-AIDS-related lymphadenopathy (34). However, there has been no conclusive evidence for active type D retrovirus infections in humans.…”

Section: Discussionmentioning

confidence: 99%

“…Extensive serological surveys in the past failed to show evidence of human type D retrovirus infection (3,17). However, a number of isolated reports have appeared over the last 15 years describing serological reactivity of human sera to type D virus antigens (22,34,35,44,45). In addition, a number of established human cell lines producing type D retroviruses have been described (11,16,24,25,37,47 cell line-derived type D isolates have been physically mapped with restriction endonucleases, and at least two of them have been molecularly cloned and sequenced.…”

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Isolation of a type D retrovirus from B-cell lymphomas of a patient with AIDS

Bohannon

Donehower

Ford

1991

J Virol

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An atypical syncytial variant of a high-grade Burkitt's-type B-cell lymphoma from a patient with AIDS who was seropositive for human immunodeficiency virus type 1 was studied. A productive type D retrovirus infection was identified in early-passage cell lines derived from two lymphomas from this patient. Nucleotide and amino acid sequence analysis as well as immunologic reactivity indicated that the isolated virus was highly related to Mason-Pfizer monkey virus (MPMV). MPMV is an immunosuppressive type D retrovirus that causes an AIDS-like syndrome in rhesus macaques. Amplification of DNA from the patient's diagnostic bone marrow biopsy specimen by polymerase chain reaction generated the appropriate MPMV-specific fragments and indicated that the patient was infected with the MPMV-like retrovirus. In addition, the patient's serum contained antibodies which recognized type D viral env proteins (gp7O and gp2O) and gag proteins (p27 and p14). Although there have been reports of human cell lines infected with type D retroviruses and of type D-reactive human sera, this is the first evidence of a type D retrovirus infection in a human confirmed by virus isolation, serum reactivity, and viral DNA identification in tumor tissue.

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“…They are capable to induce immune deficiency-like diseases in monkeys kept in Primate Research Centers [4,11]. Possibly they are associating with human immune deficiency as well [10]. The gag-coded structural proteins of type D retroviruses originate from polyprotein precursor--Pr78gag [2,7].…”

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confidence: 99%

Study of the gag-coded polyproteins of type D retroviruses Block of glycosylation inhibits the processing of major structural protein

Ilyinskii¹,

Morozov²,

Ilyin³

1990

Archives of Virology

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The study of the gag-coded glycosylated polyprotein (gPr78gag) from type D retrovirus-producing cells is reported. It is shown that glycosylation inhibitors tunicamycin and 2-deoxy-D-glucose are blocking the synthesis of gPr78gag. This demonstrates that gPr78gag is a N-glycan. The protein part of gPr78gag is supposedly identified (MW = 65 k). The inhibitors used partially stopped the processing of gag-coded polyprotein Pr78gag into core protein p27 as shown in pulse-chase experiments.

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Antibodies to structural and nonstructural gag-coded proteins of type D retroviruses in patients with lymphadenopathies

Cited by 5 publications

References 6 publications

Evidence of Infection with Simian Type D Retrovirus in Persons Occupationally Exposed to Nonhuman Primates

Evidence of Infection with Simian Type D Retrovirus in Persons Occupationally Exposed to Nonhuman Primates

Isolation of a type D retrovirus from B-cell lymphomas of a patient with AIDS

Study of the gag-coded polyproteins of type D retroviruses Block of glycosylation inhibits the processing of major structural protein

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