Antibodies to wounded tissue enhance cutaneous wound healing

Abstract

SummaryThe wound repair process is a highly ordered sequence of events that encompasses haemostasis, inflammatory cell infiltration, tissue regrowth and remodelling. Wound healing follows tissue destruction so we hypothesized that antibodies might bind to wounded tissues, which would facilitate the engulfment of damaged tissues by macrophages. Here, we show that B cells, which produce antibodies to damaged tissues, are engaged in the process of wound healing. Splenectomy delayed wound healing, and transfer of spleen cells into splenectomized mice recovered the delay in wound healing. Furthermore, wound healing in splenectomized nude mice was also delayed. Transfer of enriched B220 + cells by magnetic beads accelerated wound healing in splenectomized mice. We detected immunoglobulin G1 (IgG1) binding to wounded tissues by using fluorescein isothiocyanate-labelled anti-IgG1 6-24 hr after wounding. Splenectomy reduced the amount of IgG1 binding to wounded tissues. Immunoblotting studies revealed several bands, which were reduced by splenectomy. Using immunoprecipitation with anti-IgG bound to protein G we found that the intensity of several bands was lower in the serum from splenectomized mice than in that from sham-operated mice. These bands were matched to myosin IIA, carbamoyl-phosphate synthase, argininosuccinate synthase, actin and a-actinin-4 by liquid chromatography tandem mass spectrometry analysis.

Exploring the Bone Proteome to Help Explain Altered Bone Remodeling and Preservation of Bone Architecture and Strength in Hibernating Marmots

“…For example, we found that immunoglobulin heavy chain (a large polypeptide subunit of an antibody) was higher in bone samples in hibernating compared with active marmots. This is consistent with previous studies showing higher levels of immunoglobulin heavy chain in serum from hibernating compared with active bears (Chow et al 2013), which may possibly help explain bears' ability for wound healing during hibernation (Nishio et al 2009;Iaizzo et al 2012). Our finding is also consistent with increased numbers of B-lymphocytes in blood of hibernating 13-lined ground squirrels compared with active squirrels (Bouma et al 2013).…”

Expression of keratinocyte growth factor (KGF) and its receptor in a middle-ear cavity problem

“…Second, a large number of infiltrating B cells might be related with the pathogenesis of the cavity problem. B cells produce antibodies to damaged tissues, and are engaged in the process of wound healing [19]. As in recent observations, chronic inflammatory diseases such as the inflammatory bowel diseases and rheumatoid arthritis are characterized by a local accumulation of activated B cells [20,21,22].…”

“…However, we indicated that T lymphocytes, usually predominant during the acute inflammatory process, were thought to produce several cytokines that enhance the proliferation of epithelial cells in cholesteatoma [13,15]. On the other hand, B cells produce antibodies in damaged tissues, and are engaged in the process of wound healing [19] and some chronic inflammatory diseases [20,21,22]. In this study, we also investigated the difference in the population of B and T lymphocytes between a cavity problem and cholesteatoma.…”

Tumor-Associated and Disease-Associated Autoantibody Repertoires in Healthy Colostrum and Maternal and Newborn Cord Sera

“…The immune system is composed of complex networks of molecules and cells that act together to orchestrate the beneficial inflammation needed to maintain and repair the body as well as to protect it from neoplastic cells and invading pathogens (1)(2)(3)(4)(5)(6). Abs binding to body molecules-autoantibodieswould appear to mark the self-reactivity needed for tissue healing (7) and for tumor surveillance (8); autoantibodies also mark autoimmune diseases (9). Thus, it would be important to investigate the characteristics of autoantibodies at birth in healthy humans as a starting point for subsequent evolution of the autoreactive repertoire.…”