Antibodies with the Cell-type Specificity to the Morula Cells of the Solitary Ascidians Styela Rustica and Boltenia Echinata.

Podgornaya, O. I.; Shaposhnikova, T. G.

doi:10.1247/csf.23.349

Cited by 18 publications

(14 citation statements)

References 24 publications

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“…Acid-urea polyacrylamide gel electrophoresis (acid-urea PAGE) was performed for the mesoglein charge determination (Panyim and Chalkley, 1969;Podgornaya and Shaposhikova, 1998). AU-PAGE is sensitive to the proper charge of the proteins: only positively charged proteins, such as histones, could be separated.…”

Section: Mesoglein Extraction and Electrophoretic Analysismentioning

confidence: 99%

A novel Aurelia aurita protein mesoglein contains DSL and ZP domains

Matveev

Shaposhnikova

Podgornaya

2007

Gene

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Section: Mesoglein Extraction and Electrophoretic Analysismentioning

confidence: 99%

A novel Aurelia aurita protein mesoglein contains DSL and ZP domains

Matveev

Shaposhnikova

Podgornaya

2007

Gene

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“…3A). In all cell-containing fractions, 3 zones of 16-19 kDa could be distinguished, which probably corresponded to the histones based on their apparent mobility in SDS-PAGE (Podgornaya and Shaposhnikova, 1998). Two bottom fractions contained a large amount of 42 kDa actin.…”

Section: Polypeptide Fraction Compositionmentioning

confidence: 92%

The fate of larval flagellated cells during metamorphosis of the sponge Halisarca dujardini

Mukhina¹,

Kumeiko²,

Podgornaya³

et al. 2006

Int. J. Dev. Biol.

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Sponge larval flagellated cells have been known to form the external layer of larva, but their subsequent fate and morphogenetic role are still unclear. It is actually impossible to follow flagellated cell developmental fate unless a specific marker is found. We used percoll density gradient fractionation to separate different larval cell types of Halisarca dujardini (Demospongiae, Halisarcida). A total of 5 fractions were obtained which together contained all cell types. Fraction 1 contained about 100% FC and its polypeptide composition was very different to that of the other fractions. Of all larval cell types, flagellated cells displayed the lowest in vitro aggregation capacity. We raised a polyclonal antibody against a 68 kDa protein expressed by larval flagellated cells. Its specificity was tested on total protein extract from adult sponges by Western blotting and proved to be suitable for immunofluorescence. By means of double immunofluorescence using both this polyclonal antibody and commercial anti-tubulin antibodies, we studied the distribution of the 68 kDa protein in larval flagellated cells and its fate at successive stages of metamorphosis. In juvenile sponges just after metamorphosis the choanocytes and the upper pinacoderm were labelled with both antibodies. In larval flagellated cells, the 68 kDa protein was found all over the cytoplasm appearing as granules, while in adult sponges, it was present in the apical part of choanocytes in the vicinity of collars. Direct participation of the larval flagellated cells in the development of definitive structures was demonstrated.

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“…Mesoglein is a charged protein so acid-urea polyacrylamide gel electrophoresis (AU-PAGE; Podgornaya and Shaposhikova 1998;Waterborg 2002) was used to separate the protein for immunization. Acrylamide concentration in gels was 7 %.…”

Section: Au Electrophoresis and Antibodies Productionmentioning

confidence: 99%

Mesoglein differences between two jellyfish populations of the genus Aurelia

et al. 2015

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The medusa, Aurelia aurita (Scyphozoa, Cnidaria), is counted as a cosmopolitan species with a worldwide distribution in most seas, from the poles to the tropics. Cnidarian is thought to possess two tissue layers: endoderm (gastroderm) and ectoderm, which are separated by huge mesoglea in medusa. The main medusas' morphology is similar in different populations. Previously the new protein ''mesoglein'' was determined as one of the main components of mesoglea. Deduced amino acid sequence of mesoglein contains Zona Pellucida (ZP) domain. The comparison of mesoglein and its gene from three habitats White Sea (WsA), Black Sea (BsA), Japonic Sea (JsA) has been done in the current work. The set of the mesoglea protein bands after SDS-PAGE is similar in all samples. Nevertheless, JsA mesogleins' M r is 53\55 kDa, while WsA and BsA mesogleins have M r of 47 kDa. Antibodies raised against WsA mesoglein recognize only mesoglein with M r of 47 kDa, but not 53\55 kDa, both on immunoblot and immunocytochemistry. Mesogleal cells and ''elastic'' fibrils are stained intensively in the mesoglea both from WsA and BsA but not from JsA. The possibility of gene diversity was checked by PCR with primers specific for WsA mesoglein gene. PCR products of expected length were obtained on cDNA from polyA-RNA template from mesogleal cells of WsA and BsA medusa but not on cDNA of JsA medusa. Our results evidence that there are two different species in genus Aurelia: Aurelia aurita inhabits White and Black Seas while Aurelia sp.1 inhabits Japonic Sea. Such a suggestion is in agreement with the one previously done on the base of comparison by other molecular biology methods.

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Antibodies with the Cell-type Specificity to the Morula Cells of the Solitary Ascidians Styela Rustica and Boltenia Echinata.

Cited by 18 publications

References 24 publications

A novel Aurelia aurita protein mesoglein contains DSL and ZP domains

A novel Aurelia aurita protein mesoglein contains DSL and ZP domains

The fate of larval flagellated cells during metamorphosis of the sponge Halisarca dujardini

Mesoglein differences between two jellyfish populations of the genus Aurelia

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