Antibody Immobilization on Waveguides Using aFlow–Through System Shows Improved Listeria monocytogenesDetection in an Automated Fiber Optic Biosensor: RAPTORTM

Nanduri, Viswaprakash; Kim, Giyoung; Morgan, Mark T.; Ess, Daniel R.; Hahm, Byoung-Kwon; Kothapalli, Aparna; Valadez, Angela M.; Geng, Tao; Bhunia, Arun K.

doi:10.3390/s6080808

Cited by 48 publications

(29 citation statements)

References 18 publications

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“…This is further dependent on the type of immobilization technique used. Stronger antibodybacterial binding is indicated by lower K d values, which in turn implies higher sensitivity of the biosensor [36].…”

Section: Principle Of Operationmentioning

confidence: 99%

Magnetoelastic biosensor for the detection of Salmonella typhimurium in food products

Guntupalli

Lakshmanan

Johnson

et al. 2007

Sens. & Instrumen. Food Qual.

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In this article, a magnetoelastic sensor immobilized with polyclonal antibody for the detection of Salmonella typhimurium in food products is described. The remote query nature of magnetoelastic sensors enables the detection of bacterial species in sealed and opaque containers. Bacterial binding to the antibody on the sensor surfaces changed the resonance parameters, and these changes were quantified by the shift in the sensor's resonance frequency. Response of the sensors to increasing concentrations (5 · 10 1 -5 · 10 8 cfu/ml) of S. typhimurium in three different food products (water, fat-free milk and apple juice) was studied and similar responses were observed. These results were also further ascertained by Scanning Electron Microscopy (SEM) studies. A detection limit of 5 · 10 3 cfu/ml, with a sensitivity of 139 Hz/decade was obtained for the sensors tested in water samples, as compared to 129 Hz/decade in apple juice and 127 Hz/ decade in fat free milk. A 2 · 0.4 · 0.015 mm sensor was employed in all the investigations. The dissociation constant K d and the binding valencies for S. typhimurium spiked in water samples was 435 cfu/ml and 2.33 respectively; as compared to 309 cfu/ml and 2.38 for apple juice; and 1389 cfu/ml and 1.85 for fat free milk samples. Bacterial binding was specific and a divalent binding was observed.

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Section: Principle Of Operationmentioning

confidence: 99%

Magnetoelastic biosensor for the detection of Salmonella typhimurium in food products

Guntupalli

Lakshmanan

Johnson

et al. 2007

Sens. & Instrumen. Food Qual.

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“…In sandwich assay, the effective disassociation constant (K d ) and the binding valences for static mode of antibody immobilization in spiked PBS samples was 4×10 5 CFU/mL and 4.9 as compared to 7×10 4 CFU/mL and 3.9 for flow through method of antibody immobilization. Thus the sensitive flow-through immobilization method was used to test food samples, which could detect 5×10 5 CFU/mL of L. monocytogenes in frankfurter sample (Nanduri et al 2006). A surface plasmon resonance based immunosensor for the rapid, sensitive and selective detection of generic E. coli in water samples was developed.…”

Section: Detection Of Pathogensmentioning

confidence: 99%

Optical biosensors for food quality and safety assurance—a review

et al. 2011

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Food quality and safety is a scientific discipline describing handling, preparation and storage of food in ways that prevent food borne illness. Food serves as a growth medium for microorganisms that can be pathogenic or cause food spoilage. Therefore, it is imperative to have stringent laws and standards for the preparation, packaging and transportation of food. The conventional methods for detection of food contamination based on culturing, colony counting, chromatography and immunoassay are tedious and time consuming while biosensors have overcome some of these disadvantages. There is growing interest in biosensors due to high specificity, convenience and quick response. Optical biosensors show greater potential for the detection of pathogens, pesticide and drug residues, hygiene monitoring, heavy metals and other toxic substances in the food to check whether it is safe for consumption or not. This review focuses on optical biosensors, the recent developments in the associated instrumentation with emphasis on fiber optic and surface plasmon resonance (SPR) based biosensors for detecting a range of analytes in food samples, the major advantages and challenges associated with optical biosensors. It also briefly covers the different methods employed for the immobilization of bio-molecules used in developing biosensors.

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“…A hill plot was constructed using the dose response curves [22,28,34,35] to determine the binding kinetics (K d and n H ) (Fig. 4).…”

Section: Salmonella Enteridis Eschericia Colimentioning

confidence: 99%

Selective detection of Salmonella typhimurium in the presence of high concentrations of masking bacteria

Lakshmanan

Guntupalli

Hong

et al. 2008

Sens. & Instrumen. Food Qual.

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This article presents the results of an investigation into the ability of a magnetoelastic biosensor to detect Salmonella typhimurium, with high specificity, even in the presence of high concentrations of masking bacteria. Magnetoelastic biosensors are mass sensitive devices comprised of a magnetoelastic material that serves as the transducer and bacteriophage as the bio-recognition element. The sensors were tested individually with several different genus bacteria (Salmonella typhimurium, Salmonella entertidis, Escherichia coli O157:H7 and Listeria monocytogenes) to compare the bioprobe's affinity towards species other than the target analyte. The effect of the presence of one masking bacteria (E. coli) and two masking bacteria (E. coli and L. monocytogenes) in a mixture with S. typhimurium upon the response of the biosensor was studied. It was observed that the response of the sensors followed similar trends; however the sensitivity and the frequency changes were slightly lower for the mixtures. The sensitivity of the sensors exposed to only S. typhimurium was 161 Hz decade -1 and in mixtures with one and two masking bacteria were 131 Hz decade -1 and 127 Hz decade -1 , respectively. Binding kinetics studies show that the dissociation constant (K d ) and the binding valency (1/n H ) samples with only S. typhimurium are 149 cfu mL -1 and 2.49, respectively.

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Antibody Immobilization on Waveguides Using aFlow–Through System Shows Improved Listeria monocytogenesDetection in an Automated Fiber Optic Biosensor: RAPTORTM

Cited by 48 publications

References 18 publications

Magnetoelastic biosensor for the detection of Salmonella typhimurium in food products

Magnetoelastic biosensor for the detection of Salmonella typhimurium in food products

Optical biosensors for food quality and safety assurance—a review

Selective detection of Salmonella typhimurium in the presence of high concentrations of masking bacteria

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