Antibody response in cattle after vaccination with inactivated and attenuated rabies vaccines

Abstract

SUMMARYDespite the absence of current official reports showing the number of cattle infected by rabies, it is estimated that nearly 30,000 bovines are lost each year in Brazil. In order to minimize the important economic losses, control of the disease is achieved by eliminating bat colonies and by herd vaccination. In this study, we compare the antibody response in cattle elicited by vaccination with an attenuated ERA vaccine (AEvac) and an inactivated-adjuvanted PV (IPVvac) vaccine. The antibody titers were appraised by cell-culture neutralization test and ELISA, and the percentage of seropositivity was ascertained for a period of 180 days. IPVvac elicited complete seropositivity rates from day 30 to day 150, and even on day 180, 87% of the sera showed virus-neutralizing antibody titers (VNA) higher than 0.5IU/ml. There were no significant differences between the VNA titers and seropositivity rates obtained with IPVvac in the two methods tested. AEvac, however, elicited significantly lower titers than those observed in the group receiving inactivated vaccine. In addition, the profiles of antirabies IgG antibodies, evaluated by ELISA, and VNA, appraised by cellculture neutralization test, were slightly different, when both vaccines were compared.

Association between handling stress in the corral and rabies antibody titers in selenium-supplemented cattle

“…By the end of the study, rabies antibody titers had decreased in most of the groups (Figure 2), as expected for primo-vaccinated cattle (30)(31)(32)(33)(34). In contrast, these antibody titers were sustained in group G 3.6 that received selenium supplementation close to 3 mg/day, the dose recommended by the NRC for taurine breeds (23).…”

Interval between first dose and booster affected antibody production in cattle vaccinated against rabies

“…However, single doses of rabies vaccines have been associated with low levels of neutralizing antibodies and also with occurrence of disease in vaccinated animals (9,15). On the other hand, the use of a booster has significantly increased antibody specific levels (2,6,10,14,17).…”

Effect of the Contents and Form of Rabies Glycoprotein on the Potency of Rabies Vaccination in Cattle

“…Among them (discussed in detail by Zanetti et al1998, Rodrigues da Silva et al 2000 a point that must be raised is the fact that, because rabies vaccines are officially controlled in Brazil by tests performed on laboratory animals (mice or guinea-pigs), it is always questionable whether the minimal mandatory requirements for a vaccine being approved really correspond to high immunogenicity in the target species. We have recently reported that an attenuated ERA vaccine officially approved by the LARA evoked very low and transient titers of neutralizing antibodies in cattle (Rodrigues da Silva et al 2000). Another point to be stressed is the importance of the content of rabies glycoprotein (G) in the vaccine.…”