Antibody Response to Strain Combinations of Newcastle Disease Virus as Measured by Hemagglutination-Inhibition

Abstract

Differences in antibody response to three Newcastle disease virus (NDV) strains--B-1, LaSota, and Ulster--were investigated using the hemagglutination-inhibition (HI) micro-titer test in chickens hatched from ND-immune and unimmune flocks. When used singly as primary vaccines, the Ulster strain stimulated the lowest antibody response of the three in both immune and unimmune (susceptible) chickens. Subgroups of each of the primary-vaccinated groups were revaccinated with each of the three strains. Ulster-vaccinated chicks, revaccinated with Ulster, gave the poorest booster response. All other revaccination combinations gave a significant titer increase, though some were better than others. It is suggested that the Ulster strain as primary vaccine followed by booster does of B-1 or LaSota will induce a higher antibody response (i.e., immunity) in susceptible chicken populations with less risk of a post-vaccination reaction.

Comparative proteome analysis of tracheal tissues in response to infectious bronchitis coronavirus, Newcastle disease virus, and avian influenza virus H9 subtype virus infection

“…The allantoic fluid was clarified by centrifugation at 3000 × g for 10 min and filtered with a Teflon membrane and the presence of IBV was confirmed by negative contrast electron microscopy (JEM‐1200, EX; JEOL Ltd., Tokyo, Japan) and RT‐PCR as previously described . NDV and subtype H9 AIV viral particles in the allantoic fluids of inoculated eggs were confirmed using hemagglutination inhibition (HI) analysis with specific antibodies, respectively . The titers of the viruses were determined by inoculation at tenfold dilutions into groups of five 10‐day‐old embryonated chicken eggs.…”

Effect of Probiotics, Prebiotics, Synbiotics, Organic Acids and Enzymes Supplementation on broiler Chicks’ Immunity in relation to the Economic Performance

“…Reagents used in the HI test were prepared according to the standard microplate system described by Majiyagble and Hitchner (1977) HI test was performed as the following: Using the multichannel microtiter pipette, 50 µl of PBS-pH were dispensed in each well of the 96-well microtiter plates. 50 µl of each serum sample (all serum samples of all group) from the beginning till the end of the experiment were dispensed in the first well of plates (one column in each plate was left as RBCs control).…”

Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein

“…Newcastle disease virus (NDV) La Sota vaccine strain, infectious laryngotracheitis virus (ILTV) (Tong et al, 2001) and subtype H9 avian influenza virus (AIV) (Yu et al, 2008) were used for evaluating the cross-reactivity with the 2 mAbs. All these virus strains were propagated once in 9-11-day-old SPF embryonated chicken eggs and the presence of NDV and subtype H9 AIV viral particles in the allantoic fluids of inoculated eggs was confirmed by HI using specific antibodies, respectively (Majiyagbe and Hitchner, 1977). The ILTV was confirmed by RT-PCR as previously described (Tong et al, 2001).…”