Anticancer activity of CopA3 dimer peptide in human gastric cancer cells

Lee, Joon Ha; Kim, In-Woo; Kim, Sanghee; Yun, Eun‐Young; Nam, Sung‐Hee; Ahn, Mi-Young; Kang, Dong‐Chul; Hwang, Jae Sam

doi:10.5483/bmbrep.2015.48.6.073

Cited by 35 publications

(23 citation statements)

References 19 publications

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“…AMPs isolated from insects, including melittin, cecropin related peptides and magainins have been shown to exhibit antitumoral activity for cells derived from mammalian tumors (Lee et al, 2015b; Su et al, 2015). In the present study, HI-3 at the concentration of 40-160 μg/ml also exhibited the proliferation inhibition of CNE2 cells in a dose- and time-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Purification of Active Antimicrobial Peptides from Hermetia illucens L., and Its Effects on CNE2 Cells

Tian

Feng

Sun

et al. 2018

Preprint

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Active antimicrobial peptide HI-3 was isolated and purified from the 5th instar larvae of Hermetia illucens L., and its effects on proliferation, apoptosis and migration of nasopharyngeal carcinoma (CNE2) cells were investigated. The expressions of telomerase reverse transcriptase (hTERT) in CNE2 cells were also studied in vitro to elucidate the mechanism involved in the action of HI-3 on CNE2 cells. Results showed that three fractions (HI-1, HI-2, HI-3) were isolated from the hemolymph of H. illucens larvae. After purified by RP-HPLC, only HI-3 showed the inhibitory activities to four strains of bacteria. It was also showed that HI-3 could effectively inhibit the proliferation of CNE2 cells in a dose- and time-dependent manner. Apoptosis of CNE2 cells was observed in the treatment with 160 μg/ml HI-3, and the early apoptosis rate up to 27.59 ± 1.14%. However, no significantly inhibitory effects and apoptosis were found on human umbilical vein endothelial cells (HUV-C). Moreover, HI-3 could significantly reduce the migration ability of CNE2 cells when compared with that of the control. On the other hand, the levels of mRNA and protein of hTERT in the HI-3 treatment were all significantly lower than that of the control. Results indicated that HI-3 could inhibit the proliferation of CNE2 cells and induce the apoptosis of CNE2 cells by down-regulating the telomerase activity in CNE2 cells, while no obvious effect was occurred on HUV-C. It inferred that HI-3 is a potential anti-tumor drug with low toxicity to normal cells.Summary StatementAn active antimicrobial peptide HI-3 was isolated and purified.Inhibitory proliferation of CNE2 cells, but no effect on normal cells.A potential antitumoral drug.

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Section: Discussionmentioning

confidence: 99%

Isolation and Purification of Active Antimicrobial Peptides from Hermetia illucens L., and Its Effects on CNE2 Cells

Tian

Feng

Sun

et al. 2018

Preprint

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“…After 48 hr of treatment, cell injury in neuron cultures was quantitatively assessed by measuring the lactate dehydrogenase (LDH) activity released into the bathing medium from damaged cells, using a Cytotox 96 assay kit (Promega), according to the manufacturer’s guidelines (31) .…”

Section: Methodsmentioning

confidence: 99%

c-Jun N-terminal Kinase (JNK) induces phosphorylation of amyloid precursor protein (APP) at Thr668, in okadaic acid-induced neurodegeneration

Ahn

Kim

et al. 2016

BMB Reports

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Several lines of evidence have revealed that phosphorylation of amyloid precursor protein (APP) at Thr668 is involved in the pathogenesis of Alzheimer’s disease (AD). Okadaic acid (OA), a protein phosphatase-2A inhibitor, has been used in AD research models to increase tau phosphorylation and induce neuronal death. We previously showed that OA increased levels of APP and induced accumulation of APP in axonal swellings. In this study, we found that in OA-treated neurons, phosphorylation of APP at Thr668 increased and accumulated in axonal swellings by c-jun N-terminal kinase (JNK), and not by Cdk5 or ERK/MAPK. These results suggest that JNK may be one of therapeutic targets for the treatment of AD. [BMB Reports 2016; 49(7): 376-381]

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“…This in turn increases the membrane negative charge and transmembrane potential ( Iwasaki et al, 2009 ; Teixeira et al, 2012 ). Cancer cells also express other biomolecules which can be targeted, such as chaperone proteins HSP90 and GRP78, sialic acid ( Liu et al, 2018 ), heparin sulfate ( Lee et al, 2015 ) and O -glycosylated mucines ( Teixeira et al, 2012 ). Below, such cases will be examined.…”

Section: Nanotechnology Involvement In the Delivery Of Antimicrobial mentioning

confidence: 99%

Antimicrobial Peptides and Nanotechnology, Recent Advances and Challenges

et al. 2018

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Antimicrobial peptides are sequences of amino acids, which present activity against microorganisms. These peptides were discovered over 70 years ago, and are abundant in nature from soil bacteria, insects, amphibians to mammals and plants. They vary in amino acids number, the distance between amino acids within individual peptide structure, net charge, solubility and other physical chemical properties as well as differ in mechanism of action. These peptides may provide an alternative treatment to conventional antibiotics, which encounter resistance such as the peptide nisin applied in treating methicillin resistant Staphylococcus aureus (MRSA) or may behave synergistically with known antibiotics against parasites for instance, nisin Z when used in synergy with ampicillin reported better activity against Pseudomonas fluorescens than when the antibiotic was alone. AMPs are known to be active against viruses, bacteria, fungi and protozoans. Nanotechnology is an arena which explores the synthesis, characterization and application of an array of delivery systems at a one billionth of meter scale. Such systems are implemented to deliver drugs, proteins, vaccines, and peptides. The role of nanotechnology in delivering AMPs is still at its early development stage. There are challenges of incorporating AMPs into drug delivery system. This review intends to explore in depth, the role of nanotechnology in delivering AMPs as well as presenting the current advances and accompanying challenges of the technology.

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Anticancer activity of CopA3 dimer peptide in human gastric cancer cells

Cited by 35 publications

References 19 publications

Isolation and Purification of Active Antimicrobial Peptides from Hermetia illucens L., and Its Effects on CNE2 Cells

Isolation and Purification of Active Antimicrobial Peptides from Hermetia illucens L., and Its Effects on CNE2 Cells

c-Jun N-terminal Kinase (JNK) induces phosphorylation of amyloid precursor protein (APP) at Thr668, in okadaic acid-induced neurodegeneration

Antimicrobial Peptides and Nanotechnology, Recent Advances and Challenges

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