Anticancer Activity of Micro-Algae Extract on Human Cancer Cell Line (Mg-63)

James, Jimcy; Thomas, Jibu

doi:10.22159/ajpcr.2019.v12i1.28652

Cited by 10 publications

(3 citation statements)

References 11 publications

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“…7&8). A similar trend was observed in the cytotoxicity activity [53][54][55]. As a result of the current investigation, Myrmecia bisecta and Tetradesmus obliquus microalgae, were isolated, cultivated, and the dried microalgal biomass performed solvent extraction.…”

Section:  Antifungal Activitysupporting

confidence: 76%

Antioxidant, antimicrobial, and anticancer activity of an extract of two pollution-tolerant green microalgae

Abdelrahim,

Eladel,

Battah

et al. 2024

Benha Journal of Applied Sciences

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Microalgae are a potentially valuable source of structurally different and biologically active compounds experiencing pharmaceutical and nutraceutical value. Here we compared the antioxidant, in vitro antimicrobial activity, and anticancer potential of the widely investigated green microalga Tetradesmus obliquus and the lessstudied unicellular species Myrmecia bisecta. Based on our findings, T. obliquus extracts had much more outstanding antioxidant potentials than those of M. bisecta where IC50 values of DPPH˙ and ABTS ˙+ were 16.18 and 23.35 µg.ml -1 in T. obliquus vs. 20.21 and 26.05 µg.ml -1 in M. bisecta, respectively. Interestingly, the antibacterial activity of M. bisecta extract against the tested human pathogenic bacterial strains was more powerful than those of T. obliquus, i.e., 45.2% and 25.3% inhibition vs. 32.3% and 14.20% inhibition against Escherichia coli and Staphylococcus aureus, respectively. Both microalgal species showed significant cytotoxic effects against the HepG2 cell line with IC50 values of 36.44 and 40.12 µg.ml -1 for T. obliquus and M. bisecta, respectively. Also, both of the two microalgal species showed significant cytotoxic effects against the WI-38 cell line with IC50 values of 65.63 and 61.74 µg.ml -1 for T. obliquus and M. bisecta, respectively. Based on our results, both T. obliquus and Myrmecia bisecta are reasonable candidates for future applications in pharmaceutical manufacturing.

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Section:  Antifungal Activitysupporting

confidence: 76%

Antioxidant, antimicrobial, and anticancer activity of an extract of two pollution-tolerant green microalgae

Abdelrahim,

Eladel,

Battah

et al. 2024

Benha Journal of Applied Sciences

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“…Phytol-inhibited cell proliferation and promoted autophagy in MCF-7 and human gastric adenocarcinoma AGS cells [ 37 ]. Other constituents observed were stigmasterol chemopreventive activity in cancer [ 38 ], Ergost-5-en-3-ol [anti-cancer in gastric, lung, and ovarian cancer [ [39] , [40] , [41] , [42] ]], 3,7,11,15-Tetramethyl-2-hexadecen-1-ol [anti-cancer [ 43 ]], 9-Octadecenoic acid, 1,2,3-propanetriyl ester, (E,E,E)-compound (cytotoxic on human epidermoid larynx carcinoma (Hep-2) cell line) [ 44 ], stigmast-4-en-3-one [anti-cancer effect on the MG-63 cell line [ 45 ]].…”

Section: Discussionmentioning

confidence: 99%

Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats

Kumar

et al. 2021

Journal of Ayurveda and Integrative Medicine

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Background Colorectal cancer (CC) is the third most common cancer in the world. Annona reticulata (AR) also known as bullock's heart, is a traditional herb. AR leaf extract was initially investigated for its anti-bacterial, anti-inflammatory, anti-malarial, anti-helminthic, anti-stress, and wound healing properties. Only a few in vitro cancer studies have been conducted on AR. Although few studies have linked AR leaf extract to many cancers, comprehensive studies addressing regulation, biological functions, and molecular mechanisms leading to CC pathogenesis are clearly lacking. Objectives The present study aimed to explore the antioxidant and anti-cancer potentials of AR leaf extract in CC. Materials and methods The MTT assay was used to test the anti-proliferative activity of AR leaf extract in vitro on the HCT116 cell line. Qualitative and quantitative phytochemical characterization was carried out using gas chromatography: mass spectrometry (GC–MS). 1,2-dimethylhydrazine (DMH) was used to establish CC model in female Wistar rats. The acute toxicity of AR leaf extract was tested in accordance with OECD guidelines. Aberrant Crypt Foci (ACF) count, organ index, and hematological estimations were used to screen for in vivo anti-cancer potential. The antioxidant activity of colon homogenate was determined. Results The alcoholic leaf extract (IC50, 0.55 μg/ml) was found to be more potent than the aqueous extract. Using GC–MS, a total of 108 compounds were quantified in the alcoholic leaf extract. The LD 50 value was found to be safe at a dose of 98.11 mg/kg of body weight. AR alcoholic leaf extract significantly (p < 0.05) decreased ACF count and normalized colon length/weight ratio. AR leaf extract increased RBC, hemoglobin and platelets levels. The AR alcoholic leaf extract reduced the DMH-induced tumors and significantly (p < 0.05) increased the activity of endogenous antioxidant enzymes such as catalase, reduced glutathione, superoxide dismutase, and decreased the lipid peroxidase activity. AR leaf extract reduced the inflammation caused by DMH and helped to repair the colon's damaged muscle layers. Conclusion Based on the findings from the present study, it can be concluded that the alcoholic leaf extract of AR has antioxidant and anti-proliferative properties and can aid in the prevention of CC development and dysplasia caused by DMH.

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“…38 using the microculture tetrazolium , and compared to that of untreated controls [12]. Cells ulbecco's Minimum Essential Media which supplemented with 10% fetal bovine serum, 100 streptomycin, and 250 ng/ml BioScience, Copenhagen, Denmark).…”

Section: Moharram Et Al Int J Pharm Pharm Sci Vol 12 Issuementioning

confidence: 99%

Secondary Metabolites From Rice Culture of Aspergillus Sp. Isolated From Melaleuca Subulata (Cheel) Craven Leaves and Their Anticancer Activity

Ibrahim

Kamel

et al. 2020

Int J Pharm Pharm Sci

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Objective: Aspergillus fungus is a rich source of natural products with broad biological activities. This study was conducted to identify secondary metabolites from the rice culture of Aspergillus species isolated from Melaleuca subulata leaves and evaluated their anticancer activity. Methods: Ethyl acetate extract was fractionated on silica gel and Sephadex columns. Structures of the compounds were established using physical and chemical methods. Cytotoxic activities of the extract and pure compounds against two human cancer cell lines (Mcf-7and Hep G2) were evaluated using microculture tetrazolium assay as well as the mode of the cytotoxicity was evaluated. Molecular docking studies have been performed using the Hsp 90 enzyme as an anticancer target. Results: Methyl linoleate (1), arugosin C (2), ergosterol (3), sterigmatocystin (4), diorcinol (5), alternariol-5-O-methyl ether (6), averufin (7), averufanin (8), and alternariol (9) were identified from ethyl acetate extract. All tested compounds exhibit week activity against MCF-7 and Hep G2 cell lines but a mixture of compounds 7 and 8 is considered to be more active towards both MCF-7 and Hep G 2 in comparison to other compounds. Compound 4 exhibits moderate activity against Hep G2 only as well as the ethyl acetate extract exerts moderate activity against MCF-7 cell line Moreover, compound 4 and a mixture of 7 and 8 caused a decrease in the number of Hep G2 cancer cells due to apoptotic and necrotic processes. Most active anticancer candidates 7 and 8 showed binding to the active site similar to geldanamycin reference ligand. Conclusion: Secondary metabolites identified from Aspergillus sp. and their anticancer activity were evaluated. Molecular docking suggested active candidates as Hsp 90 inhibitors.

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Anticancer Activity of Micro-Algae Extract on Human Cancer Cell Line (Mg-63)

Cited by 10 publications

References 11 publications

Antioxidant, antimicrobial, and anticancer activity of an extract of two pollution-tolerant green microalgae

Antioxidant, antimicrobial, and anticancer activity of an extract of two pollution-tolerant green microalgae

Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats

Secondary Metabolites From Rice Culture of Aspergillus Sp. Isolated From Melaleuca Subulata (Cheel) Craven Leaves and Their Anticancer Activity

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