Anticancer Activity of Nanoemulsions Loading Biomaterial <i>Amomum kravanh</i> Oil against Oral Cancer Cells

Abstract

Several biomaterial presented anticancer activities including volatile oil. Interestingly, the volatile oil from Amomum kravanh has been reported as anticancer activity. Nevertheless, the utilization in native oil might be barrier to apply its as anticancer agent because of the limitation of their water solubility. One of the solutions to this challenge is to use nanoemulsions as a carrier for delivery A.kravanh oil (AMO) into oral cancer cell. In this research, the phase inversion temperature method was used to prepared nanoemulsions containning AMO. The physical and anticancer property of various nanoemulsions were comparatively evaluated. The 8AMO:2Soybean oil (SBO) illustrated spherical and small oil droplet around 80 nm. It also presented good physical stability after temperature cycling test. Regarding anticancer property, the 8AMO:2SBO can considerably arrest oral cancer cells with dose dependent manner at IC50 of 0.76% v/v, and the nuclear fragmentation that was remarkable feature of apoptosis was also found in 8AMO:2SBO group. This discovery demonstrated that the AMO loaded nanoemulsions system can be consider to alternative choices for oral cancer prevention and treatment.

Impact of Fixed Oil on Ostwald Ripening of Anti-Oral Cancer Nanoemulsions Loaded with Amomum kravanh Essential Oil

“…Fruits of Amomum kravanh were collected from plants harvested in Chanthaburi, Thailand. Its essential oil was produced by hydro-distillation according to a previously described method [ 3 ]. A human oral squamous cancer cell line (KON; Lot No.…”

“…They may undergo a variety of destabilizing events upon storage, in particular, Ostwald ripening [ 14 ]. With respect to the composition of the oil phase in a previous report [ 3 ], AMO without fixed oil cannot be formed into stable nanoemulsions. Ostwald ripening is a significant destabilizing phenomenon of nanoemulsions, which leads to irreversible phase separation.…”

“…It is used as a folk medicine for the treatment of digestive disorders and stomach diseases [ 1 ]. Recently, Amomum kravanh essential oil (AMO) has been shown to exhibit cytotoxic effects on various cancer cells in vitro, such as prostate, skin [ 2 ], and oral cancer cells [ 3 ]. Unfortunately, its use in native form results in a low bioavailability because of its low water solubility.…”

“…Unfortunately, its use in native form results in a low bioavailability because of its low water solubility. To overcome this problem, nanoemulsions have been used as a carrier to deliver AMO more effectively [ 3 ].…”