Anticancer, Antioxidant Properties and Phenolic, Flavonoid Composition of Heracleum platytaenium Plant Methanolic Extracts

Abstract

ABSTRACT:Heracleum platytaenium is an edible herb and strongly aromatic plant that is endemic to Turkey. Although, a number of studies have been conducted on the components and composition of essential oils of the different parts of this species, its antioxidant, antiproliferative and /or cytotoxic proporties have never been explored. The present study aimed to evaluate total phenolic content (TPC), total flavonoid content (TFC), the antioxidant activity and anticancer properties of the methanolic extracts of H. platytaenium. The results showed that the total phenolic and flavonoid contents were found to be higher in the methanolic extracts from the flowers of the plant. The methanolic flower extract of H. platytaenium scavenged about 86% and stem extract of H. platytaenium scavenged about 83% of free DPPH radicals. The best inhibitory profile for GST was observed with the methanolic extract of the plant from flowers. In addition, the methanol extract of H. platytaenium from the stem increased GPx activity with the 1.25 mg/mg plant extract. The maximum dose of the flower and stem extracts of the plant inhibited 10 % and 8% CAT activity. Then, MTT assay was used for the study of the cytotoxic effect of the plant extracts. Both methanolic flower and stem extract of H. platytaenium showed moderate anticancer effect on T47-D cell lines, but no cytotoxic effect was observed on MDA-MB-231 cells. In conclusion, methanolic extracts of H. platytaenium flower and stem showed apparent antioxidant and cytotoxic effects leading the way for their potential use as food additives to help to fight against the growth and spreading of cancerous cells. However, for a definitive conclusion, further studies on other cell lines as well as animal models and subsequent clinical studies are warranted.

The cytotoxic and apoptotic effects of wild and polyploidy genotype of Artemisia cina extracts on the WiDr colon and HTB-183 lung cancer cell lines

“…The study of Tulbaghia violacea on some cancer cell lines (HeLa, ME-180, MDA-MBA-231, and MCF-7) showed increased p53 expression until 40% on HeLa and 33% on ME180in treated cells (Astirin et al 2009). The study on methanol extract of flower and stem of H. platytaenium showed that both extracts inhibit T47-D cell lines cancer cell growth but not on MDA-MB-231 cells (Akillilar et al 2018). From both results, it appears that the extract M and TWN induced the decrease of expression of Bcl-2, a protein marker of cancer incidence followed by increased expression of protein p53, an apoptosis induction.…”

“…Herbal medicinal are usually used as sources of agents for traditional medicines for thousands of years. In recent years, natural products and their derivate became beneficial alternative therapy (Akillilar et al 2018). Artemisia is one of the well-known plants for its antimicrobial, insecticidal, antioxidant, and antimalarial ability.…”

“…and others in Indonesia (Kristiani et al 2016;Sholikhah 2016). Heracleum platytaenium and others in form Turkey (Tuzlaci and Senkardes 2011;Akillilar et al 2018), in China, included A. capillaries Thunb, A. centifolia Buch-Ham. Ex Roxb, A. japonica Thunb, etc.…”

Quantification of phenolic and flavonoid contents and some biological activities of Ornithogalum sigmoideum Freyn Sint

“…Moreover these molecules have disease-fighting potentials against several chronic and degenerative disorders including autoimmune, cardiovascular, dermatologic, neurodegenerative, ocular and respiratory system diseases and even aging and cancer [2][3][4][5]. Polyphenols and flavonoids, which are found in almost all fruits and vegetables, are natural antioxidant and anti-inflammatory compounds [6][7][8].…”

Glutathione transferase and mammary tumors

“…Екстракти Heracleum platytaenium, яка багата на фенольні сполуки та флавоноїди, інгібували активність GST на 38-47% і мали цитотоксичний ефект на клітини лінії раку молочної золози T47D (позитивна експресія рецепторів естрогену) (Telkoparan et al, 2018).…”