Anticancer L-Asparaginase and Phytoactive Compounds From Plant Solanum nigrum Against MDR (Methicillindrug resistant) Staphylococcus aureus and Fungal Isolates

Abstract

L-asparaginase is used in chemotherapy for acute lymphoblastic leukemia and other cancers. L-asparaginase derived from bacterial source triggers immune responses. The current study investigates Solanum nigrum as a novel and latent source of L-asparaginase to minimize immunological reactions. The antitumor activity of SN methanol extract was determined using the potato disc assay. InterPro Chimera and InterPro were used to predict the amino acid sequence of L-asparaginase and its anticancer activity. Purification of the enzyme was carried out to homogeneity of 1.51-fold with a recovery of 61.99%. At optimal conditions of 36.5°C, pH 8.6, and 8.5 g/mL substrate, fruit (crude extract) revealed an L-asparaginase titer of 48.23 U/mL. The molecular weight of the enzyme was calculated to be 32 ± 5 kDa using SDS PAGE. The fruit’s total flavonoids and phenolic contents are 0.42 ± .030 g/mL and 94 ± 1.9 mg CAE, respectively. Anti-tumorigenic efficacy was determined to be 66% against Agrobacterium tumefaciens. Additionally, the extract possesses potent antifungal and antibacterial properties. Molecular docking provided the structural motifs and underlying interactions between L-asparaginase, N-acetylglucosamine, murine, and chitin. SN contains high levels of the enzyme L-asparaginase and phytochemicals, making it a potential source of anticancer drugs.

Establishment of a high‐efficiency transformation and genome editing method for an essential vegetable and medicine Solanum nigrum

“…Solanum nigrum ( S. nigrum ) belongs to the Solanaceae family, along with eggplant, tomato, and potato (Edmonds & Chweya, 1997). It is a valuable leafy vegetable rich in protein and vitamins and is an essential medicinal plant applied to various cancers, neuromuscular disorders, and post‐covid complications (Aisha et al, 2022; Chen et al, 2022; Sharma et al, 2023; Srinivasan & Smolke, 2020). Furthermore, S. nigrum var.…”

Antimicrobial Activity of Rhenium Di- and Tricarbonyl Diimine Complexes: Insights on Membrane-Bound S. aureus Protein Binding

“…Within the specific context of this article, CADD has been used, e.g., to evaluate medicinal plant-derived active compounds that could be used as therapeutic alternatives for MRSA infection [17][18][19][20]. The study of receptor-ligand interactions in the framework of molecular docking has drawn attention to the importance of probing the efficiency of these plant-derived antimicrobial agents [17,18] and testing antimicrobial activity using screened lead compounds, focusing on the role of computational screening methods [20][21][22][23] in tackling the problem. However, a major strategy still pursued in the field is that of modifying already approved antibiotics [24].…”

Antimicrobial Activity of Rhenium Di- and Tricarbonyl Diimine Complexes: Insights on Membrane-Bound S. aureus Proteins Binding

“…to evaluate medicinal plants-derived active compounds that could be used as therapeutic alternatives for MRSA infection [17][18][19][20]. The study of receptor-ligand interaction in the frame of molecular docking has increased the importance of probing the efficiency of these plant-derived antimicrobial agents [17,18] and testing antimicrobial activity using screened lead compounds focusing on the role of computational screening methods [20][21][22][23] in tackling the problem. However, a major strategy still pursued in the field is that of modifying already approved antibiotics [24].…”