Anticancer Metal Complexes: Synthesis and Cytotoxicity Evaluation by the MTT Assay

Abstract

Titanium (IV) and vanadium (V) complexes are highly potent anticancer agents. A challenge in their synthesis refers to their hydrolytic instability; therefore their preparation should be conducted under an inert atmosphere. Evaluation of the anticancer activity of these complexes can be achieved by the MTT assay.The MTT assay is a colorimetric viability assay based on enzymatic reduction of the MTT molecule to formazan when it is exposed to viable cells. The outcome of the reduction is a color change of the MTT molecule. Absorbance measurements relative to a control determine the percentage of remaining viable cancer cells following their treatment with varying concentrations of a tested compound, which is translated to the compound anticancer activity and its IC 50 values. The MTT assay is widely common in cytotoxicity studies due to its accuracy, rapidity, and relative simplicity.Herein we present a detailed protocol for the synthesis of air sensitive metal based drugs and cell viability measurements, including preparation of the cell plates, incubation of the compounds with the cells, viability measurements using the MTT assay, and determination of IC 50 values. Video LinkThe video component of this article can be found at

Importance of fluorine in 2,3-dihydroquinazolinone and its interaction study with lysozyme

“…The docking results were analyzed using Accelrys discovery studio software. carried out in five replicates and percentage inhibition was calculated using the following formula [37,38].…”

Cytotoxic Vanadium Complexes of Branched [ONNO]‐Type Diamine Bis(phenolato) Ligands

“…In addition, similar experiments were performed with human ovarian A2780 cells (parent cisplatin-sensitive cells) and A2780cis cells (with acquired cisplatin resistance), in order to gain more insight into the cytotoxicity pattern of these compounds. The measurements were performed as described previously, [41] by treating the cells with 10 different concentrations of the examined compound dissolved in THF and evaluating the viability of the cells after three days of incubation by use of the methylthiazolyldiphenyltetrazolium bromide (MTT) assay. Compound 1 was administered as a stereoisomeric mixture of the cis and trans isomers.…”

Cytotoxic O-bridged inert titanium(IV) complexes of phenylenediamine-bis(phenolato) ligands

“…All salophan-Ti IV complexes were found to be highly stable, with t 1/2 above 100 h as expected for O-bridged clusters with no labile ligands [15,16,22,23,26]. The cytotoxic activity of the complexes was studied on colon HT-29 cancer cell lines, employing the methylthiazolyldiphenyltetrazolium bromide (MTT) assay [30]. Relative IC 50 values are summarized in Table 2, and cytotoxicity plots are given in Fig.…”