Anticarcinogenic activity of natural sweeteners, cucurbitane glycosides, from Momordica grosvenori

Takasaki, Midori; Konoshima, Takao; Murata, Yuji; Sugiura, Masaki; Nishino, Hoyoku; Tokuda, Harukuni; Matsumoto, Kazuhiro; Kasai, Ryoji; Yamasaki, Kazuo

doi:10.1016/s0304-3835(03)00285-4

Cited by 130 publications

(78 citation statements)

References 13 publications

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“…Accumulation of in vitro evidence demonstrating the inhibitory effects of certain natural products on liver cancer cells (Fang, Hsu, Lin, & Yen, 2010). The anti-carcinogenic activity of cucurbitane-type triterpenoids has been clearly demonstrated in two-stage carcinogenesis testing in skin tumors (Takasaki et al, 2003). The triterpenoid saponin-rich G. oldhamiana root extract (TGOE) selectively inhibited the proliferation of hepatoma SMMC-7721 cells in a dose-dependent manner, while the cytotoxic effects of TGOE on normal hepatocyte L02 cells were much lower.…”

Section: Discussionmentioning

confidence: 99%

“…Triterpenes have previously been purified from the bitter melon, and detailed analysis has shown that the compounds consist in the main of cucurbitane-type triterpenoids (Liao et al, 2012). The anti-carcinogenic activity of cucurbitane-type triterpenoids has been clearly demonstrated in two-stage carcinogenesis testing in skin tumors induced by peroxynitrite (ONOO -) as an initiator and TPA as a promoter in a specific pathogen-free mouse model (Takasaki et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Suppression of Hepatitis B Virus Production and Inflammatory Response in vitro and in vivo by Mormodica charantia Compound EMCDO

Chang¹,

Chen²,

Chen³

et al. 2015

JAS

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Eight compounds were purified from Mormodica charanti, and their chemical structures were determined in this study. Their anti-HBV and anti-inflammation activities were investigated. Compound EMCDO exhibited the most efficient effect in terms of reducing HBV surface antigen, e antigen and viral DNA levels in HBV particles or surface antigen-producing cells 2.2.15 and PLC/PRF/5, respectively. Tumor suppressor p53 played a significant role in EMCDO-mediated anti-HBV effects. Pretreatment with EMCDO prevented 2.2.15 cells-induced tumor formation in a nude mice subcutaneous model. The anti-HBV and anti-tumor activities of EMCDO were better than those of oltipraz, an inhibitor of HBV transcription. EMCDO reduced the proinflammatory cytokines and mediators in LPS-treated RAW264.7 cells in a dose-dependent manner. The LPS-upregulated phosphorylation level of I was reduced in the presence of EMCDO. The transcription activity of NF-B was increased in cells treated with EMCDO. Utilization of a mouse ear edema model further confirmed the activity of EMCDO against TPA-elicited inflammation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Suppression of Hepatitis B Virus Production and Inflammatory Response in vitro and in vivo by Mormodica charantia Compound EMCDO

Chang¹,

Chen²,

Chen³

et al. 2015

JAS

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“…7) Studies related to the carcinogenic activity of natural sweeteners isolated from Lo Han Kuo demonstrate that mogroside V and 11-oxo-mogroside V show an inhibition of peroxynitrite initiated and 12-o-tetradecanoylphorbol-13-acetate promoted tumors, through antioxidant properties. 23) On the other hand, recent reports provide evidence that oxidative states and free radical generation play important roles in mast cell activation and degranulation. 24,25) Taken together, all these findings lead us to assume that the inhibitory effects of Lo Han Kuo on nasal rubbing and scratching behavior may be due to an inhibition of histamine release from mast cells through the prevention of superoxide anion generation.…”

Section: Discussionmentioning

confidence: 99%

Effect of Lo Han Kuo (Siraitia grosvenori Swingle) on Nasal Rubbing and Scratching Behavior in ICR Mice

Hossen

Shinmei

Jiang

et al. 2005

Biological & Pharmaceutical Bulletin

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Lo Han Kuo (Siraitia grosvenori Swingle) is the fruit of the Siraitia grosvenori plant.1) This plant has been cultivated in a restricted area of the southern part of China, Guangxi Province, and only the heated and dried fruit or extract were traded to other countries such as southeast Asia, Japan and the United States of America.2) Lo Han Kuo has been used as a sweet beverage, it contains sweet components called mogrosides, which are triterpene glycosides 400 times sweeter than sucrose.3) In addition, the extract of the dried fruit has been employed as a folk medicine for pharyngitis, cough and stomach or intestinal catarrh. 4) Konoshima and Takasaki 2) found that the mogroside V contained in Lo Han Kuo might be valuable as a chemopreventive agent against chemical carcinogenesis. It has been recognized that the overproduction of nitric oxide, or NO radicals, induce mutation of genes and strongly initiate multistage carcinogenesis. 5,6) When Siratia grosvenori extract was tested on lowdensity lipoprotein (LDL) oxidation, it was found that its sweet elements reduced the atherogenic potential of LDL by inhibiting not only copper-mediated, but also human umbilical vein endothelial cell-mediated LDL oxidation in a dosedependent manner. 7) Similarly, Shi et al. demonstrated that Lo Han Kuo extract has antioxidant activity against free radicals generated by a hypoxanthine and xanthine oxidase system and Fe(II)-induced lipid peroxidation.8) It is well established that drugs having an antioxidant effect have anti-allergic effects as well, and that superoxide generation plays an important role in mast cell activation.9-11) The release of chemical mediators from tissue mast cells has been centrally implicated in a diversity of allergic and inflammatory disorders. Therefore, in the present study, the effect of Lo Han Kuo on allergic symptoms such as nasal rubbing and skin scratching behavior induced by histamine and compound 48/80, respectively, were studied in ICR mice. MATERIALS AND METHODS AnimalsFemale ICR mice (6-10 week-old) were obtained from Japan SLC, Inc., Shizuoka. The animals were housed in an air-conditioned room maintained at 24Ϯ2°C with a relative humidity of 55Ϯ15%. They were given standard laboratory rodent chow (Oriental Yeast, Tokyo) and water ad libitum. All procedures involving animals were conducted in accordance with the guidelines of the Animal Care and Use Committee, Faculty of Pharmaceutical Sciences, Okayama University.Lo Han Kuo Samples To obtain Lo Han Kuo extract, fresh Lo Han Kuo fruits was washed and crushed; then, an extraction was done using hot water at a temperature range of 80 to 90°C. After filtration, the obtained extract was evaporated under reduced pressure. The moisture content of the extract was about 40 w/w%. To prepare the Lo Han Kuo glycoside, Lo Han Kuo extract in paste form was diluted and the sweet components were selectively adsorbed onto a reversal phase column. Using an ethanolic solution, the sweet components that had been adsorbed onto the columns were eluted. Ethano...

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“…For example, SGE reduces the atherogenic potential of low-density lipoprotein (LDL) by inhibition of copper-mediated oxidation and human umbilical vein endothelial cell-mediated oxidation (Takeo et al, 2002). In particular, 11-oxo-mogroside V in SGE has been shown to inhibit the tumor-promoting activity of 12-O-tetradecanotiphorbol-13-acetate in a two-stage skin carcinogenesis model in mice and the tumor-initiating activity of peroxinitrite (Takasaki et al, 2003). Additionally, 11-oxo-mogroside V in SGE has been also reported to have inhibitory effects on reactive oxygen species (ROS) and DNA oxidative damage .…”

Section: Introductionmentioning

confidence: 99%

Suppressive effect of Siraitia grosvenorii extract on dicyclanil-promoted hepatocellular proliferative lesions in male mice

et al. 2009

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-Dicyclanil (DC) generates reactive oxygen species (ROS) due to Cyp1a1 induction, and DNA damage caused by oxidative stress is probably involved in hepatocarcinogenesis in mice. To clarify the modifying effect of the Siraitia grosvenorii extract (SGE), which has antioxidative properties, we employed a 2-stage liver carcinogenesis model in partially hepatectomized male ICR mice. Mice maintained on diet containing DC at a concentration of 1,500 ppm for 9 weeks after a single intraperitoneal injection of diethylnitrosamine (DEN) at a dose of 30 mg/kg and they were given water containing 2,500 ppm of SGE for 11 weeks including 2 weeks as pre-administration on DC. SGE inhibited the induction Cyp1a1, all of which were caused by DC. To examine whether SGE indirectly inhibits Cyp1a1 expression induced by inhibition of aryl hydrocarbon receptor (Ahr)-mediated signal transduction caused by DC, mice with high containing tap water for 2 weeks. Cyp1a1 administered DC + SGE than in C57BL/6J mice administered DC alone; there was no difference in the Cyp1a1 expression between DBA/2J mice administered DC + SGE and DC alone. These results suggest that SGE suppresses the induction of Cyp1a1, leading to inhibition of ROS generation and consequently inhibited hepatocarcinogenesis, probably due to suppression of Ahr activity.

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Anticarcinogenic activity of natural sweeteners, cucurbitane glycosides, from Momordica grosvenori

Cited by 130 publications

References 13 publications

Suppression of Hepatitis B Virus Production and Inflammatory Response in vitro and in vivo by Mormodica charantia Compound EMCDO

Suppression of Hepatitis B Virus Production and Inflammatory Response in vitro and in vivo by Mormodica charantia Compound EMCDO

Effect of Lo Han Kuo (Siraitia grosvenori Swingle) on Nasal Rubbing and Scratching Behavior in ICR Mice

Suppressive effect of Siraitia grosvenorii extract on dicyclanil-promoted hepatocellular proliferative lesions in male mice

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