Anticariogenic Activity and Haemolytic Study of Some Medicinal Plants Leaf Protein Extract against Six Oral pathogens in In vitro condition

Soni, H.; Ishnava, Kalpesh B.; Patel, Khushal

doi:10.3126/ijasbt.v2i3.9344

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…Concentration of 320, 640, 1280, 256 mg/ml against S. aureus was chosen according to the ineffective results with the used of initial dosage range 20, 40, 80, 160 mg/ml. The plates were incubated at 37°C for 24hrs until appearances of zone of inhibition [2]. The experiment was conducted in triplicate.…”

Section: Agar Well Diffusion Methodsmentioning

confidence: 99%

“…Anticariogenic is a term used to describe foods, chemicals, or other agents that tend to contribute favorably to dental health by remineralizing teeth and discouraging the acid that causes dental caries [1]. There are some medicinal plants that show antimicrobial and anticariogenic effects [2]. Genus Solanum belongs to the family Solanaceae and comprises more than 1700 species, found in the tropical and temperate zones.…”

Section: Introductionmentioning

confidence: 99%

“…The fruit of Solanum erianthum D., Solanum nigrum L., Solanum suratease L., Solanum virgianum L., Solanum xanthocarpum, as well as seeds of Solanum khasianum and Solanum torvum are claimed to have oral medicinal properties traditionally used by tribes in India [4]. Solanum suratease is also found to have antimicrobial activity [2]. The leaf extract of Solanum nigrum L. and Solanum myriacantus Dunal contain polyphenol which give them the antimicrobial and anticariogenic effects [5].…”

Section: Introductionmentioning

confidence: 99%

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Anticariogenic properties of Solanum ferox L. ethanol extract

Raduan

Khalid

Jihek

et al. 2019

ADUM

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Solanum ferox L. is a plant species which belongs to the Solanaceae family and the genus Solanum. The Solanum genus was found to exhibit anticariogenic activity and was traditionally used to treat oral diseases. However, there is no scientific study done specifically for Solanum ferox L. Hence the aim of the study is to determine the anticariogenic properties of flesh and leaf of ethanolic extract of Solanum ferox L. Alkaloids, flavonoids and tannins were detected in the leaf ethanolic extract via preliminary phytochemical screening. The presence of these phytochemicals may contribute to the anticariogenic activity. Treatment with different concentrations of flesh and leaf of ethanolic extract were used against Streptococcus pyogenes and Staphylococcus aureus via the method of agar well diffusion to indicate zones of inhibition. The antibiofilm activity of the flesh and leaf ethanolic extracts was tested. The flesh and leaf ethanolic extracts possess antimicrobial activity dose-dependently and positive antibiofilm activity against respective pathogens. The flesh ethanolic extract has stronger anticariogenic activity compared to leaf ethanolic extract against respective pathogens. Streptococcus pyogenes exhibited higher susceptibility as compared to Staphylococcus aureus. In conclusion, it has been shown that the ethanolic extract of Solanum ferox L. exhibit anticariogenic properties against Streptococus pyogenes and Staphylococcus aureus.

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Section: Agar Well Diffusion Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anticariogenic properties of Solanum ferox L. ethanol extract

Raduan

Khalid

Jihek

et al. 2019

ADUM

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“…Prepare polyacrylamide gel (12%) according to standard protocol followed by Hiral et al, (2014) [27]. Load samples and run gel at 25 mA (2 gels run at 50 mA) in 1x SDS running buffer.…”

Section: Acacia Nilotica Crude Protein Profiling By Sds-pagementioning

confidence: 99%

Antimicrobial and Hemolytic Activity of Seed Protein Extracts from Selected Medicinal Plants against Tooth Decaying Microorganisms

2020

International Journal of Research Studies in Microbiology and B

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All oral diseases, the incidence of those that have a microbial etiology is greatest in all parts of the world. In the present study, the antimicrobial sensitivity assay of plant seed protein extracts against tooth decaying organisms was carried out. 2 out of 10 plant seed protein extracts show their activity against 4 out of total 6 selected oral infection causing bacteria. Inhibition zones against Lactobacillus acidophilus of Acacia nilotica seed protein extract is 16mm and Lactobacillus acidophilus and Staphylococcus aureus; the inhibition zone is 4mm against Cassia occidentalis. MIC value for Lactobacillus acidophilus and Staphylococcus aureus against Acacia nilotica and Cassia occidentalis seed protein extract is 0.0463 µg/ml and 0.115µg/ml respectively. The protein content of Acacia nilotica and Cassia occidentalis seed protein extract is 3.801mg/ml and 3.842mg/ml respectively. Hemolytic activity of Acacia nilotica seed protein has HC 50 value of 1.58 mg. Kinetic studies of Lactobacillus acidophilus and Staphylococcus aureus the organisms clearly indicate the kinetic of inhibition against both the organisms with respect to time. Treatment with proteinase K of the Acacia nilotica seed protein results in absence of the inhibition zone which clearly indicates the activity is only due to protein. Acacia nilotica seed protein extract maximum protein band present in 40µl sample and 11 protein band are present. Hemolytic activities of the Acacia nilotica plant seed protein gives HC 50 value at a very high range than that of the MIC values against both of the organisms and thus flourish its application as successful pharmaceutical drug in practice.

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