Anticlastogenic activity exhibited by botryosphaeran, a new exopolysaccharide produced by Botryosphaeria rhodina MAMB-05

Miranda, Carolina C.B.; Dekker, Robert F. H.; Serpeloni, Juliana Mara; Fonseca, Eduardo Borges da; Cólus, I.M.S.; Barbosa, Aneli M.

doi:10.1016/j.ijbiomac.2007.10.010

Cited by 59 publications

(24 citation statements)

References 44 publications

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“…A botriosferana é uma β-glucana produzida pelo fungo ascomiceto Botryosphaeria rhodina MAMB-05 que demonstrou atividade anticlastogênica (MIRANDA et al, 2008). Algumas β-glucanas que apresentam atividade biológica possuem baixa solubilidade em água e elevada viscosidade, dificultando o desenvolvimento de testes biológicos.…”

Section: Obtenção De Oligossacarídeos Bioativosunclassified

beta-(1,3)-Glucanases Fúngicas: Produção e Aplicações Biotecnológicas.

Bauermeister¹,

Rezende²,

Giese³

et al. 2010

Semina: Tech. Ex.

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Resumoβ-1,3-Glucanases são enzimas que hidrolisam ligações glicosídicas do tipo β-1,3 presentes em β-D-glucanas, liberando glucose como produto principal. Estas enzimas são produzidas por fungos filamentosos e leveduriformes, assim como bactérias, apresentando amplas aplicações biotecnológicas. O objetivo do presente trabalho foi revisar a literatura publicada sobre β-1,3-glucanases fúngicas, enfatizando-se suas funções nos micro-organismos, alguns parâmetros utilizados para a determinação da atividade enzimática, substratos utilizados e condições de cultivo para a sua produção. Também foram relatadas aplicações destas enzimas na obtenção de oligossacarídeos bioativos, na caracterização da parede celular de micro-organismos, no controle biológico de patógenos de algumas plantas, como aditivos em rações para animais, além de sua adição em vinhos para melhorar as características organolépticas. Palavras-chave: Enzimas. β-glucanas. Fungos filamentosos. Leveduras. Abstractβ-1,3-Glucanases are enzymes that hydrolyze glycosidic linkages of the β-1,3 type present in β-Dglucans, liberating glucose as the main product. These enzymes are produced by filamentous fungi and yeasts, as well as bacteria and have wide biotechnological applications. The objective of this work was to review the published literature on fungal β-1,3-glucanases, emphasizing their biological functions, some parameters used to determine enzyme activity, the current substrates and culture conditions to produce these hydrolases. Also reported are their applications to obtain bioactive oligosaccharides, to structurally characterize microbial cell walls, their use in biological control of some plant pathogens, as feed additives, and also their addition to wines to improve the organoleptic characteristics.

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Section: Obtenção De Oligossacarídeos Bioativosunclassified

beta-(1,3)-Glucanases Fúngicas: Produção e Aplicações Biotecnológicas.

Bauermeister¹,

Rezende²,

Giese³

et al. 2010

Semina: Tech. Ex.

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“…This outcome is different from our experiment. The difference in monosaccharides and the presence of glucuronic acid in our shiitake sample should be caused by variation in variety of shiitake sample and the analytical condition employed [20,21]. …”

Section: Hplcmentioning

confidence: 99%

Analysis of chemical components of shiitake polysaccharides and its anti-fatigue effect under vibration

Zhang

2009

International Journal of Biological Macromolecules

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“…Microbial Exopolysaccharides (EPS) have been used by chemical, pharmaceutical and food sectors as bio-flocculants, bio-adsorbents, agents to remove heavy metals, for control-release of drugs and as gelling, stabilizer and food thickening agents (Wang et al, 2008;Schultheis et al, 2009). Some of these EPS's and especially the fungal β-D-glucans, present biological activities, which have been demonstrated to possess antioxidant (Schultheis et al, 2009), antimutagenic (Cho et al, 2006), antiviral (Miranda et al, 2008), anticoagulant and antithrombotic (Yuan et al, 2009), hypoglycemic and hypocholesterolemic (Miranda-Nantes et al, 2011) properties, as well as exhibiting an anti-proliferative effect on breast cancercells . These pharmacological properties confer on these biomacromolecules important applications as nutraceuticals and present a promising market for new commercial products.…”

Section: Introductionmentioning

confidence: 99%

Exocellular (1→6)-β-D-Glucan (Lasiodiplodan): Carboxymethylation, Thermal Behavior, Antioxidant and Antimicrobial Activity

Theis¹,

Calegari²,

Santos³

et al. 2017

American Journal of Immunology

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β-Glucans are biomacromolecules of industrial interest for its biological and technological properties, including ability to modify the rheology of food systems and different biological functionalities. In this study, lasiodiplodan an exocellular polysaccharide of the (1→6)-β-Dglucan type produced by the fungus Lasiodiplodia theobromae MMPI was obtained in a bench-scale bioreactor operated in discontinuous mode. Lasiodiplodan produced was derivatized by carboxymethylation and characterized by Fourier Transform Infrared spectroscopy (FT-IR), Thermogravimetry and Differential Thermal Analysis (TG-DTA), X-Ray Diffraction (XRD), scan electron microscopy (SEM) and their antioxidant and antimicrobial potential also was assessed. Two strong absorption bands in the regions of 1422 to 1598 cm −1 , resulting from symmetric and asymmetric stretching vibrations of the -COO-group respectively, were observed in the FT-IR spectrum of the derivatized sample and indicated the carboxymethylation of the macromolecule. TG-DTA curves indicated that native (LAS-N) and carboxymethylated (LAS-C) lasiodiplodan did not suffer significant changes in relation to the heating rates. Native and carboxymethylated lasiodiplodan demonstrated high thermal stability considering the usual standards of the pharmaceutical industry. The final temperature of thermal decomposition increased when the heating rate was increased and less number of steps for thermal decomposition was observed when air was substituted for nitrogen. XRD analysis showed the LAS-N and LAS-C have no crystalline structure, but carboxymethylation led to the arising of regions with certain molecular orientation in biopolymer structure. SEM analysis showed that the carboxymethylation promoted changes in biopolymer macrostructure, including breaking of the polymeric structure and arising of bubbles on the surface area. Carboxymethylation contributed to improving the polysaccharide's antioxidant capacity and LAS-C demonstrated antimicrobial activity against Candida tropicalis.

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Anticlastogenic activity exhibited by botryosphaeran, a new exopolysaccharide produced by Botryosphaeria rhodina MAMB-05

Cited by 59 publications

References 44 publications

beta-(1,3)-Glucanases Fúngicas: Produção e Aplicações Biotecnológicas.

beta-(1,3)-Glucanases Fúngicas: Produção e Aplicações Biotecnológicas.

Analysis of chemical components of shiitake polysaccharides and its anti-fatigue effect under vibration

Exocellular (1→6)-β-D-Glucan (Lasiodiplodan): Carboxymethylation, Thermal Behavior, Antioxidant and Antimicrobial Activity

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Anticlastogenic activity exhibited by botryosphaeran, a new exopolysaccharide produced by Botryosphaeria rhodina MAMB-05

Cited by 59 publications

References 44 publications

beta-(1,3)-Glucanases Fúngicas: Produção e Aplicações Biotecnológicas.

beta-(1,3)-Glucanases Fúngicas: Produção e Aplicações Biotecnológicas.

Analysis of chemical components of shiitake polysaccharides and its anti-fatigue effect under vibration

Exocellular (1&rarr;6)-&beta;-D-Glucan (Lasiodiplodan): Carboxymethylation, Thermal Behavior, Antioxidant and Antimicrobial Activity

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Exocellular (1→6)-β-D-Glucan (Lasiodiplodan): Carboxymethylation, Thermal Behavior, Antioxidant and Antimicrobial Activity