Anticoagulant Polyethylene Terephthalate Surface by Plasma-Mediated Fucoidan Immobilization

Abstract

Biomaterial-based blood clot formation is one of the biggest drawbacks of blood-contacting devices. To avoid blood clot formation, their surface must be tailored to increase hemocompatibility. Most synthetic polymeric biomaterials are inert and lack bonding sites for chemical agents to bond or tailor to the surface. In this study, polyethylene terephthalate was subjected to direct current air plasma treatment to enhance its surface energy and to bring oxidative functional binding sites. Marine-sourced anticoagulant sulphated polysaccharide fucoidan from Fucus vesiculosus was then immobilized onto the treated polyethylene terephthalate (PET) surface at different pH values to optimize chemical bonding behavior and therefore anticoagulant performance. Surface properties of samples were monitored using the water contact angle; chemical analyses were performed by FTIR and X-ray photoelectron spectroscopy (XPS) and their anticoagulant activity was tested by means of prothrombin time, activated partial thromboplastin time and thrombin time. On each of the fucoidan-immobilized surfaces, anticoagulation activity was performed by extending the thrombin time threshold and their pH 5 counterpart performed the best result compared to others.

Furcellaran Surface Deposition and Its Potential in Biomedical Applications

“…The typical surface modification techniques used include chemical vapor deposition, wet chemical methods, UV light radiation, ozone-induced treatment and plasma exposure. Among the physical and chemical methods used to obtain required surfaces without affecting the bulk properties, plasma treatment is an appropriate and efficient technique [27][28][29]. In addition to the efficiency of immobilization, plasma treatment is able to enhance hydrophilicity and create a high density of functional groups on the surface without the use of toxic chemicals and heat processing.…”

Biomaterials from the sea: Future building blocks for biomedical applications

“…The thrombin time threshold was prolonged when contacting with the fucoidan-modified surfaces. It is presumed that these fucoidan-modified surfaces may serve as the alternatives as anti-clotting devices such as blood storage bags [ 391 ]. Chitosan-fucoidan nanoparticles possess better in vitro anticoagulant activity than pristine fucoidan.…”

X-ray Ptychographic Imaging and Spectroscopic Studies of Plasma-Treated Plastic Films

“…Therefore, chemical [3], enzymatic [4] and physical modifications have been carried out to make the PET surface more hydrophilic. There are several methods to treat polymeric surfaces, such as wet chemistry, UV irradiation, corona discharge, flame treatment and ozone treatment [4][5][6][7][8]. Fundamental and applied research on these processing methods has been conducted in recent decades [2,3,7,9] to study physical and chemical modification processes on the polymer surface.…”