Anticorrosive effects and in vitro cytocompatibility of calcium silicate/zinc-doped hydroxyapatite composite coatings on titanium

Huang, Yong; Zhang, Honglei; Qiao, Hongchao; Nian, Xiaofeng; Zhang, Xuejiao; Wang, Wendong; Zhang, Xiaoyun; Chang, Xiaotong; Han, Shuguang; Pang, Xin

doi:10.1016/j.apsusc.2015.10.034

“…Then, the dehydration of the samples was done with a graded series of alcohol for 15 minutes; thereafter, they were dried in supercritical CO 2 . 7,8 The samples were then sputter-coated with gold, and the cell morphology was observed with the SEM. To observe the cytoskeleton, the adhering cells were immersed in a 4% paraformaldehyde solution for 10 minutes and were then stained with phalloidin (5 μg mL −1 ; Sigma-Aldrich Co., St Louis, MO, USA).…”

Section: Cell Morphologymentioning

confidence: 99%

“…5 The HA coatings on metal implants can provide a suitable substrate for the growth of bone; such coatings not only allow for tight chemical bonds at the bone/implant interface but also can act as a barrier between body fluids and the metallic implant. 6 The in vivo success of a coated implant depends on several factors; the surface topography, that is, surface roughness and porosity, can strongly influence biological processes like protein adsorption, cell adhesion, and cell spreading; 7 moreover, the proliferation of osteoblasts, vascular ingrowth, and the subsequent bone formation can be further enhanced by an interconnected porous coating layer; 8 the interconnected porousness of the coating may make the implant suffer from bacterial activity in the early stages after the implantation, which may require additional medical operations for retrieving the implant. 9 In addition, HA is faced with an intrinsically high dissolution rate in a biological environment and poor corrosion resistance in acid solutions, 10 which is detrimental for long-term implant stability.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured Ag<sup>+</sup>-substituted fluorhydroxyapatite-TiO<sub>2</sub> coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications

Huang

¹

,

Song

²

,

Chang

³

et al. 2018

IJN

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BackgroundPoor mechanical properties, undesirable fast dissolution rate, and lack of antibacterial activity limit the application of hydroxyapatite (HA) as an implant coating material. To overcome these limitations, a hybrid coating of Ag+-substituted fluorhydroxyapatite and titania nanotube (TNT) was prepared.MethodsThe incorporation of silver into the HA-TiO2 hybrid coating improves its antimicrobial properties. The addition of F as a second binary element increases the structural stability of the coating. The TNT/F-and-Ag-substituted HA (FAgHA) bilayer coating on the Ti substrate was confirmed by X-ray diffraction, scanning electron microscope, energy-dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS).ResultsThe results indicate that the FAgHA/TNT nanocomposite coating has a dense and uniform morphology with a nano-rod-like structure. The solubility measurement result shows that the substitution of F− ions into the AgHA structure has a positive effect on the dissolution resistance of HA. The adhesion strength of FAgHA/TNT has significantly increased because of the interlocking of the roughened surface with nano-rod-like particles that entered into the voids of the TiO2 nanotubes. Compared with that of the bare Ti, the corrosion current density of FAgHA/TNT-coated Ti substrate decreased from 3.71 to 0.18 μA, and its corrosion resistance increased by almost two orders of magnitude. Moreover, despite pure HA, the FAgHA killed all viable Staphylococcus aureus after 24 hours of incubation. Although the fabricated FAgHA/TNT coating is hydrophobic, it induced deposition of the typical spherical apatite when immersed in a simulated body fluid (SBF); the osteoblasts spread very well on the surface of the coating. In addition, in vitro cell culture tests demonstrated cell viability and alkaline phosphatase (ALP) similar to pure HA, which indicated good cytocompatibility. Interestingly, compared with bare Ti, FAgHA/TNT-coated Ti surface was innocent for cell vitality and even more beneficial for cell osteogenesis in vitro.ConclusionEnhancing the osseointegration and preventing infection in implants, the FAgHA/TNT-coated Ti makes implants more successful.

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“…In addition, recent studies showed that M. luteus, a Gram-positive bacteria, commonly found in mucous membranes, was highly inhibited by nanoHAp and doped nano-ZnHAp [42]. On the other hand, Huang et al [43] showed in their study on "anticorrosive effects and in vitro cytocompatibility of calcium silicate/zinc doped hydroxyapatite composite coatings on titanium" that the high cytocompatibility of calcium silicate/zinc doped hydroxyapatite could be mainly attributed to the combination of microporous surface effects and ion release (Zn…”

Section: Journal Of Nanomaterialsmentioning

confidence: 99%

Structural and Biological Assessment of Zinc Doped Hydroxyapatite Nanoparticles

Popa

¹

,

Aur

²

,

Deniaud

³

et al. 2016

Journal of Nanomaterials

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The aim of the current research work was to study the physicochemical and biological properties of synthesized zinc doped hydroxyapatite (ZnHAp) nanoparticles with Zn concentrations Zn = 0 (HAp), Zn = 0.07 (7ZnHAp), and Zn = 0.1 (10ZnHAp) for potential use in biological applications. The morphology, size, compositions, and incorporation of zinc into hydroxyapatite were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR), Raman scattering, and X-Ray Photoelectron Spectroscopy (XPS). In addition, the cytotoxicity of ZnHAp nanoparticles was tested on both E. coli bacteria and human hepatocarcinoma cell line HepG2. The results showed that ZnHAp nanoparticles (HAp, 7ZnHAp, and 10ZnHAp) have slightly elongated morphologies with average diameters between 25 nm and 18 nm. On the other hand, a uniform and homogeneous distribution of the constituent elements (calcium, phosphorus, zinc, and oxygen) in the ZnHAp powder was noticed. Besides, FTIR and Raman analyses confirmed the proper hydroxyapatite structure of the synthesized ZnHAp nanoparticles with the signature of phosphate, carbonate, and hydroxyl groups. Moreover, it can be concluded that Zn doping at the tested concentrations is not inducing a specific prokaryote or eukaryote toxicity in HAp compounds.

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“…In addition, recent studies showed that M. luteus, a Gram-positive bacteria, commonly found in mucous membranes, was highly inhibited by nano-HAp and doped nano-ZnHAp [42]. On the other hand, Huang et al [43] showed in their study on "anticorrosive effects and in vitro cytocompatibility of calcium silicate/zinc doped hydroxyapatite composite coatings on titanium" that the high cytocompatibility of calcium silicate/zinc doped hydroxyapatite could be mainly attributed to the combination of microporous surface effects and ion release (Zn 2+ and Si 4+ ). The Zn-Ag-HAp nanoparticles were found to be compatible with in vitro experiments and having potential antibacterial properties and could be a promising candidate for future biomedical applications [44].…”

Section: Resultsmentioning

confidence: 99%

In vitro cytocompatibility and corrosion resistance of zinc-doped hydroxyapatite coatings on a titanium substrate

Ding

¹

,

Zhang

²

,

Huang

³

et al. 2014

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The aim of the current research work was to study the physicochemical and biological properties of synthesized zinc doped hydroxyapatite (ZnHAp) nanoparticles with Zn concentrations Zn = 0 (HAp), Zn = 0.07 (7ZnHAp), and Zn = 0.1 (10ZnHAp) for potential use in biological applications. The morphology, size, compositions, and incorporation of zinc into hydroxyapatite were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR), Raman scattering, and X-Ray Photoelectron Spectroscopy (XPS). In addition, the cytotoxicity of ZnHAp nanoparticles was tested on both E. coli bacteria and human hepatocarcinoma cell line HepG2. The results showed that ZnHAp nanoparticles (HAp, 7ZnHAp, and 10ZnHAp) have slightly elongated morphologies with average diameters between 25 nm and 18 nm. On the other hand, a uniform and homogeneous distribution of the constituent elements (calcium, phosphorus, zinc, and oxygen) in the ZnHAp powder was noticed. Besides, FTIR and Raman analyses confirmed the proper hydroxyapatite structure of the synthesized ZnHAp nanoparticles with the signature of phosphate, carbonate, and hydroxyl groups. Moreover, it can be concluded that Zn doping at the tested concentrations is not inducing a specific prokaryote or eukaryote toxicity in HAp compounds.

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Anticorrosive effects and in vitro cytocompatibility of calcium silicate/zinc-doped hydroxyapatite composite coatings on titanium

Cited by 31 publications

References 43 publications

Nanostructured Ag<sup>+</sup>-substituted fluorhydroxyapatite-TiO<sub>2</sub> coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications

Nanostructured Ag<sup>+</sup>-substituted fluorhydroxyapatite-TiO<sub>2</sub> coatings for enhanced bactericidal effects and osteoinductivity of Ti for biomedical applications

Structural and Biological Assessment of Zinc Doped Hydroxyapatite Nanoparticles

In vitro cytocompatibility and corrosion resistance of zinc-doped hydroxyapatite coatings on a titanium substrate

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