Antidermatophytic, the anticancer and antioxidant activity of Cassia alata ethanolic extract and its phytochemical analysis

Sujatha, J.; Rajeshkumar, S.; Thangavelu, Lakshmi

doi:10.26452/ijrps.v10i2.260

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…A low susceptibility level was observed for T. mentagrophyte, T. tonsurans, T. rubrum, M. audouinii and M. gypseum. This agrees with Sujatha and Asokan (2018), who reported the antifungal activity of hexane extract of Cassia alata leaves against strains of M. canis, T. mentagrophytes, T. rubrum, E. floccosum, and M. audouinii but with varying concentrations.…”

Section: Resultssupporting

confidence: 92%

Phytochemical screening and antifungal activity of Cassia alata (Linn.) crude leaf extracts

Emmanuel,

Martin-Luther,

Adetunji

et al. 2023

Afr. J. Microbiol. Res.

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The antimycotic effect of Casia alata (Linn.) commonly used for the treatment of skin diseases by local people in Nigeria was evaluated against dermatophytes (Trichophyton tonsurans, Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum audouinii, Microsporum canis, Microsporum gypseum, Epidermophyton floccosum). Ten-fold serially diluted extract concentrations were subjected to phytochemical screening and the antimycotic activity of the phytochemicals was evaluated via broth microdilution. The percentage yield of crude aqueous, n-hexane, chloroform, ethanol, and methanol leaf extracts of Cassia alata Linn was 5.6, 5.7, 1.6, 4.8, and 2.8%, respectively. Important phytochemicals including glycosides, anthraquinone, proteins, cardiac glycosides, steroids, alkaloids, phlabotannin, phenols, saponins, tannins, flavonoids, and carbohydrates were present in all the extracts. All the extracts inhibited the dermatophytes but at varying degrees. Similar minimum inhibitory concentration (MIC) values ranging from 1.56 to 3.125 mg/mL each for n-hexane and chloroform leaf extracts and MIC of 0.78 to 1.56 mg/mL each for ethanol and methanol leaf extracts were estimated. The minimum fungicidal concentration (MFC) of 6.25 mg/mL, 3.125 to 6.25 mg/mL, 1.56 to 6.25 mg/mL, 0.78 to 1.56 mg/mL for n-hexane, chloroform, ethanol, and methanol leaf extracts were observed and all the extracts were maximally active against E. floccosum. In conclusion, the potent anti-dermatophytic effect of the leaf extract of Casia alata Linn may be driven by their numerous phytochemicals, which justify its traditional uses for the treatment of skin diseases and could considerably illicit interest for the development of new drug leads.

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Section: Resultssupporting

confidence: 92%

Phytochemical screening and antifungal activity of Cassia alata (Linn.) crude leaf extracts

Emmanuel,

Martin-Luther,

Adetunji

et al. 2023

Afr. J. Microbiol. Res.

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“…Natural antioxidants from plants and microorganism have been known to act as potent preventive therapy for treating reactive oxygen species related to various ailments like diabetes mellitus, cardiovascular and neurodegenerative diseases and aging (Sujatha & Asokan, 2017). The antiradical activity of the isolated endophytic fungi and host organs extracts were evaluated for their capacity to scavenge the DPPH radical.…”

Section: Free Radical Scavenging Ac Vitymentioning

confidence: 99%

Phenolic composition and biological activity of endophytic fungi isolates inhabited Acacia nilotica

Kheir,

Elhussein,

El hassan

et al. 2023

NRFHH

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This study aimed to screen the phenolic profile, antimicrobial and antiradical activities of endophytic fungi residing in the tissues of Acacia nilotica grown in Sudan. Isolates of endophytic fungi were isolated from fruit, leaf and twig. Methanolic extracts were prepared by maceration. Antimicrobial activity was determined by the disc diffusion method. Antiradical activity was evaluated by the 1, 1-diphenyl-2-picryl hydrazyl (DPPH) assay. Ten endophytic fungi were recovered from the twig (6), leaf (2) and fruit (2) of A. nilotica. Extracts revealed better antifungal activity, with isolates T3, L1 and F1 exerting the highest inhibition zones (20.6, 20 and 18.3 mm, respectively) against Aspergillus niger. Isolates T4 and T5 revealed the highest antiradical effect with IC50 values of 302 and 478 µg/mL respectively. Chemical analysis revealed that gallic acid and naringenin were the dominant compounds. Their pattern divided the endophytic fungi into two categories; gallic acid- rich endophytes including isolates L1 and L2 (isolated from the leaf) as well as isolates T4 and T2 (isolated from the twig). The other category was naringenin- rich endophytes comprised isolates F1 and F2, which were isolated from the fruit, and isolates T1, T3, T5 and T6 isolated from the twig. Moreover, extracts accumulated gallic acid were devoid of naringenin and the opposite was true. This behavior was not detected in the host plant where these two compounds coexist and identified in all extracts.

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“…Hassan et al [ 8 ] stated that the active compound of C. alata leaf extract inhibits fungal cell walls due to the formation of pores in cells and leakage of cytoplasmic constituents. A study by Edegbo et al [ 9 ] showed that n-hexane extract has higher antifungal activity than aqueous, chloroform, methanol, and ethanol extracts and is most active against Epidermophyton floccosum and M. canis , while Sujatha et al [ 10 ] reported that the n-hexane extract of C. alata leaves has antifungal activity against M. canis, M. audouinii, T. mentagrophytes, T. rubrum , and E. floccosum at various concentrations. The chloroform extract of C. alata leaves was also reported to have antifungal activity against fungal isolates, including E. floccosum, M. gypseum , and T. mentagrophyte [ 9 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Cassia alata L.: A Study of Antifungal Activity against Malassezia furfur, Identification of Major Compounds, and Molecular Docking to Lanosterol 14-Alpha Demethylase

Saptarini,

Mustarichie,

Hasanuddin

et al. 2024

Pharmaceuticals

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Empirically, in Indonesia, the leaves of Cassia alata L. (candle bush or ketepeng cina) have been used as a topical antifungal agent. Malassezia furfur is a natural microorganism found in the human body. It is among the factors contributing to conditions such as pityriasis versicolor, a common, benign, superficial fungal infection of the skin that is closely associated with seborrheic dermatitis and dandruff. This study aimed to explore C. alata leaves, starting from determining antifungal activity against M. furfur and the identification of major compounds in the ethyl acetate and n-hexane fractions, and then we carried out molecular docking of the major compounds in the n-hexane fraction to lanosterol 14-alpha demethylase. The method was the disc diffusion technique to test antifungal activity, LC-MS/MS for major compound identification, and homology modeling through Swiss Models for molecular docking. The fractions of ethyl acetate and n-hexane extract showed concentration-dependent antifungal activity against M. furfur. The LCMS/MS analysis revealed five major compounds in the ethyl acetate and n-hexane fractions. The molecular docking demonstrated the highest binding affinity with stearidonic acid at −7.2 kcal/mol. It can be concluded that the compounds in the n-hexane fraction have antifungal activity against M. furfur, as supported by both in vitro and in silico studies.

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Antidermatophytic, the anticancer and antioxidant activity of Cassia alata ethanolic extract and its phytochemical analysis

Cited by 4 publications

References 16 publications

Phytochemical screening and antifungal activity of Cassia alata (Linn.) crude leaf extracts

Phytochemical screening and antifungal activity of Cassia alata (Linn.) crude leaf extracts

Phenolic composition and biological activity of endophytic fungi isolates inhabited <i>Acacia nilotica</i>

Cassia alata L.: A Study of Antifungal Activity against Malassezia furfur, Identification of Major Compounds, and Molecular Docking to Lanosterol 14-Alpha Demethylase

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