Antidiabetic, antioxidant, molecular docking and HPTLC analysis of miquelianin isolated from Euphorbia schimperi C. Presl

Abstract

The present study demonstrates the miquelianin or quercetin 3-O-glucuronide (compound 1) isolated from aerial parts of Euphorbia schimperi exhibited significant results for antioxidant and antidiabetic potential. The compound 1 along with kaempferol 3-O-glucuronide (compound 2) and quercetin 3-O-rhamnoside (compound 3) isolated from the same source were quantified by validated HPTLC method. Antioxidant activity was determined by chemical means in terms of ABTS radical cation and DPPH radical scavenging activity. Compound 1 showed significant scavenging activity in both ABTS and DPPH assays as compared to standard BHA. In ABTS method IC50 values of compound 1 and standard BHA is found to be 58.90 ± 3.40 µg/mL and 28.70 ± 5.20 µg/mL respectively while in DPPH assay IC50 values of Compound 1 and standard BHA is 47.20 ± 4.90 µg/mL and 34.50 ± 6.20 µg/mL respectively. Antidiabetic effect was studied through α-amylase and α-glucosidase inhibitory activity. The mechanistic approach through molecular modelling also support the strong binding sites of compound 1 which showed significant α-amylase and α-glucosidase inhibitory activities with IC50 values 128.34 ± 12.30 and 89.20 ± 9.20 µg/mL respectively as compared to acarbose 64.20 ± 5.60 and 52.40 ± 4.60 µg/mL respectively. The results of validated RP-HPTLC analyses revealed the concentration of compound 1 found to be 16.39 µg/mg and for compound 2 and compound 3 as 3.92 and 14.98 µg/mg of dried extract, respectively.

Simultaneous Determination of 6-Shogaol and 6-Gingerol in Various Ginger (Zingiber officinale Roscoe) Extracts and Commercial Formulations Using a Green RP-HPTLC-Densitometry Method

“…In this study, the green mobile phase was obtained by the simple mixture of ethanol and water (green solvents) in comparison to the normal HPTLC method. The application of RP-HPTLC methods offer several advantages over normal phase HPTLC methods [ 37 , 40 ]. In addition, green RP-HPTLC methods for the simultaneous determination of 6-SHO and 6-GIN also reduce the toxicity of the proposed method to the environment [ 35 , 36 ].…”

Ecofriendly densitometric RP-HPTLC method for determination of rivaroxaban in nanoparticle formulations using green solvents

“…Moreover, it helped in avoiding the interference due to impurities in the chromatograms, formation of compact spot and detection clarity. 37 Eco-friendly RP-HPTLC method for the analysis of rivaroxaban will reduce the environmental toxicity caused by routine toxic solvents used in routine pharmaceutical analysis. 33,34 Our research work gave a very good analytical technique for the estimation of the rivaroxaban in NPs formulation.…”

Saururus chinensis-controlled allergic pulmonary disease through NF-κB/COX-2 and PGE2 pathways

“…Recent research has shown that the major components in SC are miquelianin, quercetin 3- O -(2″- O - β-glucopyranosyl)-α-rhamnopyranoside, and quercitrin ( Nho et al, 2019 ). Miquelianin is the major consitutent of SC water-extract (11.75 ± 0.09 mg/g) and it has anti-inflammatory ( Nho et al, 2019 ), anti-oxidative, anti-diabetic ( Ahmed et al, 2019 ), and neuroprotective effects ( Yuan et al, 2017 ). Rutin is one of the most well-known herbal constituents and is used therapeutically for the suppression of atopic dermatitis ( Choi & Kim, 2013 ), and as an anti-asthmatic ( Jung et al, 2007 ).…”