Antifouling Properties of Bacteria Associated with Marine Oyster Crassostrea Sp.

Viju, Nadarajan; Punitha, S.; Satheesh, Sathianeson

doi:10.1007/s41208-018-0095-9

Cited by 9 publications

(4 citation statements)

References 77 publications

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“…The presence of soluble biocide species, such as tributyltin, extensively used from1950s, was revealed as quite dangerous for the marine environment, with local extinction of some species and bioaccumulation. Thus, strict environmental and health restrictions (issued by International Maritime Organization in 2001, with the International Convention on the Control of Harmful Antifouling Systems on Ships becoming effective in 2008) imposed a move toward alternative compounds, such as rosin (from coniferous trees), extracts of antagonist bacteria, and tin-free resins [ 95 ].…”

Section: Enhancement Of Antimicrobial Properties Via Nanomodification Of Epoxiesmentioning

confidence: 99%

Improving the Antimicrobial and Mechanical Properties of Epoxy Resins via Nanomodification: An Overview

et al. 2021

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The main purpose of this work is to provide a comprehensive overview on the preparation of multifunctional epoxies, with improved antimicrobial activity and enhanced mechanical properties through nanomodification. In the first section, we focus on the approaches to achieve antimicrobial activity, as well as on the methods used to evaluate their efficacy against bacteria and fungi. Relevant application examples are also discussed, with particular reference to antifouling and anticorrosion coatings for marine environments, dental applications, antimicrobial fibers and fabrics, and others. Subsequently, we discuss the mechanical behaviors of nanomodified epoxies with improved antimicrobial properties, analyzing the typical damage mechanisms leading to the significant toughening effect of nanomodification. Some examples of mechanical properties of nanomodified polymers are provided. Eventually, the possibility of achieving, at the same time, antimicrobial and mechanical improvement capabilities by nanomodification with nanoclay is discussed, with reference to both nanomodified epoxies and glass/epoxy composite laminates. According to the literature, a nanomodified epoxy can successfully exhibit antibacterial properties, while increasing its fracture toughness, even though its tensile strength may decrease. As for laminates—obtaining antibacterial properties is not followed by improved interlaminar properties.

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Section: Enhancement Of Antimicrobial Properties Via Nanomodification Of Epoxiesmentioning

confidence: 99%

Improving the Antimicrobial and Mechanical Properties of Epoxy Resins via Nanomodification: An Overview

et al. 2021

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“…For this, the bacterial suspension was serially diluted and spread around the Zobell marine agar (ZMA) plate (HIMEDIA, India) and the plate was then put away in a sterile chamber (at room temperature for 24 h) for the cultivation of bacterial colonies. Subsequently, the cultivated bacterial colonies were isolated individually using the quadrant streak plate method described by Viju et al (2018). The isolated bacterial colonies were classified based on their morphological characters and reserved on ZMA slant for further studies.…”

Section: Gastropod Collection and Isolation Of Their Bacterial Symbiontsmentioning

confidence: 99%

“…The bioactive bacteria were grown in the marine bacterial culture medium (ZMB) for a period of 5 days at room temperature (28 ± 1). After that, the bacterial cell pellet was separated from the culture medium by centrifugation and soaked in methanol for 72 h and the metabolites were extracted using the method explained by Viju et al (2018). Following this, the antibacterial activity of the extract of symbiotic bacteria (ESB) was tested against biofilm-forming bacterium Alteromonas sp.…”

Section: Isolation Of Bioactive Bacteriamentioning

confidence: 99%

“…Following 24 h of incubation, the EPS synthesized by Alteromonas sp. on the ZMB medium was isolated using the method outlined by Viju et al (2018). Subsequently, the carbohydrate and protein concentration of the isolated EPS was analysed by the procedures proposed by Dubois et al (1956) and Lowry et al (1951) respectively.…”

Section: Influence Of Esb On the Synthesis Of Eps By Biofilmforming Bmentioning

confidence: 99%

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Antibiofilm activity of symbiotic Bacillus species associated with marine gastropods

Viju¹,

Punitha²,

Satheesh

2020

Ann Microbiol

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Purpose: Generally, symbiotic marine bacteria are renowned for the synthesis of compounds with bioactive properties, and this has been documented in many previous studies. Therefore, the present study was aimed to isolate novel bacterial symbionts of gastropods that have the ability to synthesize bioactive compounds. These bioactive compounds could be used effectively as antibiofilm agents in order to overcome the problems associated with biofilm. Methods: The bacteria associated with the surface of marine gastropods were isolated and characterized. Following this, the bacterial metabolites were extracted and their antibiofilm effect was evaluated on biofilm-forming bacteria on artificial substrates. Moreover, the biofilm-forming bacterium Alteromonas sp. was treated with the extracts of symbiotic bacteria in order to evaluate the influence of extracts over the synthesis of extracellular polymeric substance (EPS). Besides, the biologically active chemical constituents of the extracts were separated using thin-layer chromatography and subjected to gas chromatography and mass spectrometry (GC-MS) analysis for characterization. Results: Three bacterial strains belonging to the species Bacillus firmus, Bacillus cereus and Bacillus subtilis were identified from the bacterial community associated with the gastropods. The antibiofilm assays revealed that the extract of three symbiotic bacteria significantly (p < 0.05) reduced the biofilm formation by the marine bacterium Alteromonas sp. on artificial materials. Also, the EPS synthesis by Alteromonas sp. was significantly inhibited due to symbiotic bacterial extract treatment. The chemical composition of the bioactive fraction isolated from the symbiotic bacteria extract revealed that most of the detected compounds were belonging to aromatic acid, fatty acid and carboxylic acid. Conclusion: The results of this study clearly revealed that the bacteria belonging to the above listed Bacillus species can be considered as a promising source of natural antibiofilm agents.

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An Analysis of Biosynthesis Gene Clusters and Bioactivity of Marine Bacterial Symbionts

2021

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Antifouling Properties of Bacteria Associated with Marine Oyster Crassostrea Sp.

Cited by 9 publications

References 77 publications

Improving the Antimicrobial and Mechanical Properties of Epoxy Resins via Nanomodification: An Overview

Improving the Antimicrobial and Mechanical Properties of Epoxy Resins via Nanomodification: An Overview

Antibiofilm activity of symbiotic Bacillus species associated with marine gastropods

An Analysis of Biosynthesis Gene Clusters and Bioactivity of Marine Bacterial Symbionts

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