Antigen detection ELISA: A sensitive and reliable tool for the detection of active infection of surra

Rudramurthy, Gudepalya Renukaiah; Sengupta, P. P.; Ligi, M.; Rahman, Muhammad Habibur

doi:10.1016/j.actatropica.2018.07.018

Cited by 2 publications

(1 citation statement)

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“…The CATT/T.evansi remains as the gold standard test for detection of surra as per Office International des Epizooties (OIE) but the sensitivity of the test is compromised (Songa and Hamers 1988). Recombinant protein based indirect ELISA has been widely used for detection of the carrier animals (Rudramurthy et al 2018;Sengupta et al 2014Sengupta et al , 2016. These tests can be used for conducting epidemiological studies and also to monitor the effective implementation of control strategies against trypanosomosis.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Trypanosoma evansi in cattle and analysis of associated climatic risk factors in Mizoram, India

et al. 2020

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Surra, a haemoprotozoan parasitic disease even in subclinical form poses a challenge in terms of diagnosis and management to animal health practitioners and policy makers as well; eventually imparting financial loss to the livestock holders. A systematic study was designed to assess the seroprevalence of surra in cattle and associated climatic risk factors, by collecting 480 serum samples across the eight districts of Mizoram during 2017-2019. The apparent and true seroprevalence detected by card agglutination test was 37.08% (CI at 95%: 32.88-41.49) and 36.59% (CI at 95%: 32.4-40.99) whereas by recombinant Variable Surface Glycoprotein based indirect ELISA was 41.88% (CI at 95%: 37.5-46.3) and 40.35% (CI at 95%: 36.02-44.76) respectively. Climate parameters which influence vector population were extracted from their respective database and were correlated with seroprevalence data. Linear discriminant analysis revealed that air temperature, relative humidity and diurnal temperature range, leaf area index and soil moisture as significant risk factors discriminating seropositive and seronegative data sets classified by indirect ELISA. This study is the first report on seroprevalence of surra in cattle of Mizoram and the situation demands deployment of intervention strategies in order to assess the endemicity of the disease and thereby preventing the economic losses.

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Section: Introductionmentioning

confidence: 99%