Antigen detection in human respiratory Coronavirus infections by monoclonal time-resolved fluoroimmunoassay

Hierholzer, John C.; Halonen, Pekka; Bingham, P G; Coombs, R. A.; Stone, Y O

doi:10.1016/0928-0197(94)90020-5

Cited by 8 publications

(7 citation statements)

References 42 publications

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“…Antigenic alterations have been demonstrated between the prototype strain of enterovirus 70, isolated in 1971, and strains isolated in the 1980s (33). Chimeric plasmids have recently been prepared containing a substitution of a 405-bp fragment of cDNA from coxsackievirus B3 into the 5 noncoding region of poliovirus type 1.…”

Section: Properties Of the Enterovirusesmentioning

confidence: 99%

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My Role in the Discovery and Classification of the Enteroviruses

Melnick

1996

Annu. Rev. Microbiol.

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The enteroviruses constitute one of the genera of the picornavirus family. The genus includes the polioviruses, the coxsackieviruses, and the echoviruses of humans, plus a number of enteroviruses of lower animals (e.g. monkeys, cattle, pigs, mice). Over 100 serotypes are recognized, of which the first to be discovered were the polioviruses. It was my good fortune to have been a scientist during the golden age of virology, when new techniques were being introduced into the field. These often led to the discovery of new viruses. This article details the isolation of the enteroviruses, their recognition as a separate genus of Picornaviridae, and my role in the process. Poliovirus, the most hazardous of the group, is almost gone from the world, but the other enteroviruses will be with us for some time. Several members of the Committee dealing with these agents-Enders, Sabin, Dalldorf, Syverton-have passed on, but the work of this Committee to which I was privileged to contribute will live long. CONTENTS

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Section: Properties Of the Enterovirusesmentioning

confidence: 99%

“…Incubation periods are relatively short (12-72 hr). The virus replicates preferentially at [33][34][35] • C, an adaptation to conjunctival temperatures instead of the higher temperatures of the gut.…”

Section: Properties Of the Enterovirusesmentioning

confidence: 99%

My Role in the Discovery and Classification of the Enteroviruses

Melnick

1996

Annu. Rev. Microbiol.

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“…When compared with monoclonal-and polyclonal-based ELISA assays, it was a more sensitive procedure than either: 100% of known positive nasopharyngeal aspirates were identified with the assay whereas only 69% of 229E infections and 90% of OC43 infections were diagnosed by the monoclonal-based ELISA. The sensitivity of the assay was determined as 0.308 ng virus for 229E and 0.098 ng virus for OC43 [65].…”

Section: Time-resolved Fluoroimmunoassaymentioning

confidence: 99%

Coronaviruses

Siddell¹,

Myint²

1996

Viral and Other Infections of the Human Respiratory Tract

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“…The coronaviruses are best identified directly in nasal and throat specimens by FA, EIA, and TR-FIA because the viruses are extremely labile and difficult to recover in the laboratory. The peplomers constitute the primary antigen detected in all tests, including the HI test for strain OC-43 (Hierholzer et al, 1994a).…”

Section: Cell Culturesmentioning

confidence: 99%

Advances in the diagnosis of respiratory virus infections

Halonen

Herholzer

Ziegler

1996

Clinical and Diagnostic Virology

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The choice of diagnostic methods for respiratory virus infections depends on the type and location of the laboratory, the number of specimens tested, and the previous experience of the laboratory. Virus culture, whenever possible, should be the basic diagnostic method; the results, including identification of the virus, should be available no more than 24 h later than the results of rapid diagnostic tests. In small laboratories, especially in hospitals where specimen transportation is well organized, immunofluorescence may be the best choice for antigen detection with the provision that an experienced microscopist and a good UV microscope are available. If the laboratory receives a large number of specimens and has previous experience with EIAs, then biotin-EIAs or TR-FIAs may be the most practical techniques. Their advantages include the stability of the antigens in clinical samples since intact, exfoliated epithelial cells are not required, treatment of specimens is practical, testing of large numbers of specimens is possible, and reading the printed test result is less subjective than reading fluorescence microscopy. The larger role of PCR in the diagnosis of respiratory virus infections depends on future developments such as practical methods to extract DNA or RNA and to purify the extracts from nonspecific inhibitors, plus further improvements to minimize cross-contamination. Group-specific detection of enteroviruses and rhinoviruses is an example of the potential for PCR technology. In experienced laboratories. EIA IgG antibody tests should be available. Recombinant antigens may be a useful part of such assays.

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Antigen detection in human respiratory Coronavirus infections by monoclonal time-resolved fluoroimmunoassay

Abstract: This rapid and sensitive test appears to be much more sensitive than traditional antigen detection assays but will require more extensive field testing on clinical specimens.

Cited by 8 publications

References 42 publications

My Role in the Discovery and Classification of the Enteroviruses

My Role in the Discovery and Classification of the Enteroviruses

Coronaviruses

Advances in the diagnosis of respiratory virus infections

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