Antigen-Induced Expression of CD203c on Basophils Predicts IgE-mediated Wheat Allergy

Tokuda, Reiko; Nagao, Mizuho; Hiraguchi, Yukiko; Hosoki, Koa; Matsuda, Tukasa; Kouno, K.; Morita, Eishin; Fujisawa, Takao

doi:10.2332/allergolint.08-oa-0023

Cited by 71 publications

(40 citation statements)

References 27 publications

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“…The sensitivity and specificity of antigen-induced CD203c expression were reported to be 75 and 100%, respectively, in the diagnosis of latex allergy [8]. More recently, Tokuda et al [16] and Ocmant et al [17] published papers on the utility of food antigen-induced CD203c expression in the diagnosis of FA. Ocmant et al [17] reported that sensitivity and specificity of ovalbumin (OVA)-induced BATs applied to diagnose egg allergy were 62.5 and 96.4, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, BATs may be beneficial in the diagnosis of FA. In the study by Tokuda et al [16], measurement of purified native ω–5 gliadin-induced enhancement of CD203c on basophils is useful for the diagnosis of immediate wheat allergy in children. In the present study, we evaluated whether CD203c expression on basophils induced by food antigen would be useful in the prediction of OFC-associated symptoms in hen’s egg and milk allergies.…”

Section: Introductionmentioning

confidence: 99%

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Basophil Activation Marker CD203c Is Useful in the Diagnosis of Hen’s Egg and Cow’s Milk Allergies in Children

Sato¹,

Tachimoto²,

Shukuya³

et al. 2010

Int Arch Allergy Immunol

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Background: The diagnosis of food allergy (FA) is usually based on oral food challenge tests (OFC). However, OFCs occasionally induce severe adverse reactions. CD203c expression on basophils is emerging as a potential diagnostic index. We evaluated whether CD203c expression on basophils would be a useful marker of OFC-associated symptoms in hen’s egg and cow’s milk allergies in children. Methods: Seventy-one patients who had been diagnosed with FA based on OFCs or a convincing history of FA symptoms in the Department of Pediatrics, Sagamihara National Hospital, were recruited. CD203c expression was assessed after stimulation with antigens (egg white, ovomucoid, milk or casein) using allergenicity kits. The CD203c stimulation index (SI = the allergen-induced CD203c expression level divided by the baseline expression level) and the threshold of CD203c expression (the minimum concentration of antigen to induce CD203c SI ≧2) were analyzed in association with tolerance acquisition. Results: For the CD203c SI, the areas under the receiver-operating characteristic curve were 0.72 for egg white, 0.82 for ovomucoid, 0.84 for milk and 0.67 for casein. The positive predictive value for the threshold of CD203c expression was 94.7% for egg white, 100% for ovomucoid, 85.7% for milk and 75.0% for casein. Conclusions: Assessment of food antigen-induced CD203c expression on basophils is useful to determine if children will outgrow FA as well as in decision making regarding whether or not to perform OFCs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Basophil Activation Marker CD203c Is Useful in the Diagnosis of Hen’s Egg and Cow’s Milk Allergies in Children

Sato¹,

Tachimoto²,

Shukuya³

et al. 2010

Int Arch Allergy Immunol

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“…Basophil activation, in addition to activation of mast cells and other immune cells, and subsequent production of chemical mediators (such as histamine) can contribute to the initiation of anaphylaxis [12]. Basophils express the surface activation markers CD11b, CD63, CD123, CD203c and CD294, some of which previously have been shown to reflect allergen-dependent activation of basophils ex vivo [9,13,14,15,16,17,18,19]. …”

Section: Introductionmentioning

confidence: 99%

Basophil CD203c Levels Are Increased at Baseline and Can Be Used to Monitor Omalizumab Treatment in Subjects with Nut Allergy

Gernez

Tirouvanziam

et al. 2010

Int Arch Allergy Immunol

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Rationale: Basophils contribute to anaphylaxis and allergies. We examined the utility of assessing basophil-associated surface antigens (CD11b/CD63/CD123/CD203c/CD294) in characterizing and monitoring subjects with nut allergy. Methods: We used flow cytometry to analyze basophils at baseline (without any activation) and after ex vivo stimulation of whole blood by addition of nut or other allergens for 2, 10, and 30 min. We also evaluated whether basophil expression of CD11b/CD63/CD123/CD203c/CD294 was altered in subjects treated with anti-IgE monoclonal antibody (omalizumab) to reduce plasma levels of IgE. Results: We demonstrate that basophil CD203c levels are increased at baseline in subjects with nut allergy compared to healthy controls (13 subjects in each group, p < 0.0001). Furthermore, we confirm that significantly increased expression of CD203c occurs on subject basophils when stimulated with the allergen to which the subject is sensitive and can be detected rapidly (10 min of stimulation, n = 11, p < 0.0008). In 5 subjects with severe peanut allergy, basophil CD203c expression following stimulation with peanut allergen was significantly decreased (p < 0.05) after 4 and 8 weeks of omalizumab treatment but returned toward pretreatment levels after treatment cessation. Conclusions: Subjects with nut allergy show an increase of basophil CD203c levels at baseline and following rapid ex vivo stimulation with nut allergen. Both can be reduced by omalizumab therapy. These results highlight the potential of using basophil CD203c levels for baseline diagnosis and therapeutic monitoring in subjects with nut allergy.

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“…FA involves a double blind placebo controlled food challenge. It requires a control setting as the test has the potential to be dangerous [144,145].…”

Section: Functional Assays (Fa) or Food Challenge Testmentioning

confidence: 99%

“…FA involves a double blind placebo controlled food challenge. It requires a control setting as the test has the potential to be dangerous [144,145].Currently, the management of WA is based on avoidance of wheat. Patients should undergo dietary advice and referrals to identify relevant food allergens [146].…”

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confidence: 99%

Gluten and the Gut: A Brief Review of Gluten-Related Gastrointestinal Disorders

Shah¹

2017

SOJI

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We conducted online electronic searches (published manuscripts in English) of the Cochrane Library, pub med and manual searches of selected specialty journals to identify any pertinent literature. The search was conducted using the key words 'celiac disease', 'gluten related disorder', 'non celiac gluten sensitivity', 'wheat allergy', 'gluten', , 'gluten free diet'. Studies not published in English or published only in the abstract form were excluded. We also conducted a focused review of available references to identify subsequent pertinent papers. AbstractCeliac disease (CD) was the first gluten-related disorder to be described. Presently the realm of gluten-related disorders also includes non-celiac gluten sensitivity (NCGS) and wheat allergy (WA). CD is a multisystem immune mediated enteropathy observed in genetically susceptible individuals exposed to dietary gluten present in barley, rye and wheat. Although the prevalence of CD is on the rise, it still largely remains under diagnosed given that many cases can be silent, subtle or atypical. The Pathophysiology of CD includes both innate and adaptive immune response to dietary gluten. Patients present with a wide variety of intestinal and extra-intestinal symptoms and are at risk for non-Hodgkin's lymphoma and small intestinal adenocarcinoma. The corner stone for treatment of CD is a gluten-free diet (GFD). NCGS is an emerging condition associated with gluten ingestion and the diagnosis of which is based on exclusion of CD and WA. Further evidence and studies are required to fully appreciate the clinical entity of NCGS and WA to detail its epidemiology and pathogenesis. The symptoms of NCGS and WA may overlap with CD and hence it is imperative to differentiate these clinical entities and select patients for appropriate management.Keywords: Celiac disease, gluten related disorder, non-celiac gluten sensitivity, wheat allergy, gluten, epidemiology, clinical features, Pathophysiology, management, gluten free diet.Core tip: CD is largely under-diagnosed while the incidence and prevalence of gluten-related disorders are on the rise. A clear understanding of the various clinical presentations of gluten-related disorders can help differentiate CD from NCGS and WA. The management for these conditions differs and hence accurate diagnosis leads to improved patient outcomes with avoidance of longterm complications as well as unnecessary testing which can be both invasive and expensive. Celiac DiseaseThe term celiac is derived from the Greek word "koiliaks" meaning "abdominal". It was first described in the second century in modern day Turkey by a Greek physician Aretaeus of Cappadocia [1]. In 1888, Samuel Gee; a pediatrician from the United Kingdom gave the first modern-day description of the condition and associated diet to the treatment of celiac disease EpidemiologyCeliac disease occurs in about 1% of the general population worldwide [7]. Data from the USA suggests CD to be more common in Caucasians as compared to African-Americans and Hispanics [8,9]. The initial ...

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Antigen-Induced Expression of CD203c on Basophils Predicts IgE-mediated Wheat Allergy

Abstract: Measurement of nOG-induced enhancement of CD203c on basophils is useful for the diagnosis of immediate wheat allergy in children.

Cited by 71 publications

References 27 publications

Basophil Activation Marker CD203c Is Useful in the Diagnosis of Hen’s Egg and Cow’s Milk Allergies in Children

Basophil Activation Marker CD203c Is Useful in the Diagnosis of Hen’s Egg and Cow’s Milk Allergies in Children

Basophil CD203c Levels Are Increased at Baseline and Can Be Used to Monitor Omalizumab Treatment in Subjects with Nut Allergy

Gluten and the Gut: A Brief Review of Gluten-Related Gastrointestinal Disorders

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