Antigen presentation and regulatory functions of human monocytes in the <i>in vitro</i> response of lymphocytes against purified protein derivative of tuberculin (PPD)

Treves, Abraham J.; Tal, Tzachy; Barak, Vivian; Fuks, Zvi

doi:10.1002/eji.1830110609

Cited by 14 publications

(4 citation statements)

References 38 publications

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“…The activity of this standard IL 1 was similar in all the tests and, therefore, we chose to express the results as the actual cpm values obtained by the tested supernatants and not by units of activity. (b) Proliferative responses of human lymphocytes: PBM, or MO-depleted PBM were tested for their response against purified protein derivative of tuberculin (PPD) as described [13]. All blood donors for these experiments were positive by a standard Mantoux skin test.…”

Section: Biological Testing Of Ilmentioning

confidence: 99%

“…In some experiments, supernatants were tested for their direct mitogenic activity by using the same culture conditions, but omitting the antigen or mitogen. MO depletion was performed by consecutive removal of plastic-and nylon wool-adherent as well as iron carbonyl phagocytic cells as described [13]. Such depleted cell preparations contained &l% of MO, as measured by phagocytosis of cell latex particles [13].…”

Section: Biological Testing Of Ilmentioning

confidence: 99%

“…MO depletion was performed by consecutive removal of plastic-and nylon wool-adherent as well as iron carbonyl phagocytic cells as described [13]. Such depleted cell preparations contained &l% of MO, as measured by phagocytosis of cell latex particles [13]. (c) Enhancement of [3H]dThd uptake by fibroblasts: the IL 1 preparations were tested for their activity on a human fibroblast cell line designated Arzi (obtained from the Department of Pediatrics, Hadassah University Hospital, Jerusalem), using the method described by Schmidt et al [14].…”

Section: Biological Testing Of Ilmentioning

confidence: 99%

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Constitutive secretion of interleukin 1 by human monocytes

et al. 1983

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The constitutive and lipopolysaccharide (LPS)-induced secretion of interleukin 1 (IL 1) by cultured human monocytes and macrophages has been studied. Both freshly obtained monocytes and their culture-derived macrophages were induced by LPS to secrete similar amounts of IL 1. Such induction, however, was accompanied by the secretion of dialyzed inhibitory activity. Constitutive secretion of IL 1 was detected in concentrated supernatants of monocyte cultures. The factor obtained constitutively did not manifest significant inhibitory activity. A method is described for the recovery of IL 1-containing supernatants in serum- and other stimulant-free medium. The biological activities of the constitutively secreted IL 1 were similar to the LPS-induced activities. The constitutive secretion of IL 1 was not equally distributed in the entire monocyte population. We found that a small fraction of loosely adherent monocytes secreted higher amounts of IL 1 than the strongly adherent monocytes. However, the property of higher secretion of IL 1 was not stable and disappeared following monocyte cultivation. Thus, constitutive activity of IL 1 could be recovered either by concentrating the culture supernatants or by enriching a subset of monocytes with higher IL 1 activity.

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Section: Biological Testing Of Ilmentioning

confidence: 99%

Section: Biological Testing Of Ilmentioning

confidence: 99%

Section: Biological Testing Of Ilmentioning

confidence: 99%

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Constitutive secretion of interleukin 1 by human monocytes

et al. 1983

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“…Although cells of the monocyte/macrophage lineage are the most significant of the APC (Rosenthal & Shevach, 1973;Treves et al, 1981), other cells types, e.g. dendritic cells (Nussenzweig et al, 1980; Kaye, Chain & Feldman, 1985) and B cells (Gerrard & Fauci, 1982;Issekutz, Chu & Geha, 1982), have been described to perform the same function.…”

Section: Introductionmentioning

confidence: 99%

Requirement of the T cell receptor for antigen presentation by T lymphocytes. Effect of envelope glycoproteins of HIV-1 on antigen presentation by T cells

Chirmule

Kalyanaraman

Slade

et al. 1990

Clinical and Experimental Immunology

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SUMMARYWe have developed CD4+, tetanus antigen-specific T cell clones that proliferate in the presence of tetanus antigen and autologous irradiated peripheral blood leucocytes (PBL) as antigen-presenting cells (APC). There have been several reports that T cells can present antigen themselves. We have used tetanus antigen-specific T cell clones to examine the effects of envelope glycoproteins of HIV-1 on processing and presentation of antigen to T cells. Cloned T cells were pre-incubated with soluble crude preparation of tetanus antigen for 4 h at 37 C, irradiated, and used as APC (T-APC). These cells could present antigen, as assessed by the ability of the autologous cloned T cells to proliferate. Resting T cells and phytohaemagglutinin-activated T cell blasts from autologous PBL could not present tetanus antigen to the responder cloned T cells. Antigen presentation by T-APC was abrogated by treating cells with anti-HLA-DR but not by anti-HLA-DQ monoclonal antibodies; treatment of tetanus antigen-pulsed T-APC with anti-tetanus antibody also blocked the ability of these cells to induce proliferation in responder T cells. Antigen presentation by cloned T cells was by a chloroquine-resistant pathway. Pretreatment of T-APC with envelope glycoprotein of HIV-1, gp 120, did not affect the proliferative responses of the responder T cells. These data suggest that gpl20 does not inhibit the antigen-presenting function while suppressing antigen-specific responses.

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Selection and continuous growth of antigen‐specific human T cells by antigen‐treated monocytes

et al. 1983

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Antigen-specific human T lymphocytes were selected by adsorption of peripheral blood mononuclear cells on monolayers of antigen-treated autologous monocytes. The enriched cell populations were propagated in interleukin 2-containing medium for 60-80 days. Their activity was tested by a proliferative response against the sensitizing antigen in the presence of irradiated autologous lymphocytes. Continuous cultures of enriched T cells responded specifically to three different protein antigens. The specific reactivity induced in vitro against at least one of the antigens was probably a primary response. In addition, the selected cultures were found to be enriched for OKT4-positive cells, in contrast to cultures of nonselected T cells which were enriched for OKT8-positive cells. These properties of the selected T lymphocyte populations were obtained following only one exposure to the sensitizing antigen, which was concomitant with adsorbance on monocytes and without cloning of the reactive cells. We suggest that specific adsorbance of lymphocytes on antigen-treated monocytes may result in a T cell population which combines both properties of antigen specificity and enrichment for the helper proliferative subset of T cells.

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Antigen presentation and regulatory functions of human monocytes in the in vitro response of lymphocytes against purified protein derivative of tuberculin (PPD)

Cited by 14 publications

References 38 publications

Constitutive secretion of interleukin 1 by human monocytes

Constitutive secretion of interleukin 1 by human monocytes

Requirement of the T cell receptor for antigen presentation by T lymphocytes. Effect of envelope glycoproteins of HIV-1 on antigen presentation by T cells

Selection and continuous growth of antigen‐specific human T cells by antigen‐treated monocytes

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