Antigenic analysis of Rhodococcus equi preparations using different horse sera

Immune response to vaccines based upon the VapA protein of the horse pathogen, Rhodococcus equi, in a murine model

“…This observation may be due to a difference in lipid modification of VapA in the two bacterial species (Tan et al, 1995). Similar results have been observed by others (Fontanals et al, 1997;Sekizaki et al, 1995).…”

“…However, none of these vaccines have been developed for use as a widely available commercial vaccine at the time of writing (Lopez et al, 2003;Fontanals et al, 1997;Prescott et al, 1997). In this study, DNA and protein vaccines based upon VapA were tested in the murine model.…”

Performance of Five Serological Assays for Diagnosis ofRhodococcus equiPneumonia in Foals

“…Fresh isolates of virulent R. equi were grown in dialysates of brain heart infusion broth for 30 days at 37°C. Bacteria were removed by high-speed centrifugation, and the resultant supernatant consisted of a concentrated composite of soluble R. equi antigens, including exoenzymes, cell wall components, capsular polysaccharides, and virulence-associated proteins, that served as a polyvalent antigen in the assay (6). Positive control serum samples were collected from naturally occurring microbiologically confirmed cases of R. equi-induced bronchopneumonia (3,7).…”