Antigenic Competition in IgE Antibody Production

Abstract

An adoptive cell transfer system was utilized to evaluate the site of action of the suppressive mechanism involved in antigenic competition in IgE antibody production. Carrier-primed (OA) and hapten-primed (DNP-KLH) spleen cells were transferred to syngeneic irradiated recipients that were challenged with a heterologous conjugate (DNP-OA). To study the effect of antigenic competition on T and B cells, donor mice of one or the other cell type received in addition the competitor antigen (Asc) at immunization. The adoptive secondary IgE anti-DNP antibody response was suppressed in both situations. This effect could not be attributed to transfer of Asc-primed cells. Irradiation of donor mice before immunization with the two antigens abrogated the suppressive effect. These results indicate that both Th and B cells primed to the test antigen were affected by antigenic competition.

Application of computer-assisted molecular modeling for immunoassay of low molecular weight food contaminants: A review

“…Many factors can affect antibody production, such as the selection of adjuvant, the amount of antigen, and the complex response of the immune system [81][82][83][84][85][86][87][88][89]. The knowledge of the three-dimensional conformation of the antigen is also extremely Fig.…”