Antigenic Differences Among Pestiviruses

Edwards, S.; Paton, David

doi:10.1016/s0749-0720(15)30467-9

Cited by 18 publications

(26 citation statements)

References 77 publications

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“…Pestiviruses are small enveloped, positive-sense RNA viruses (4,5) which naturally infect swine and domestic and wild ruminants (6). Pestiviruses display considerable genetic and antigenic diversity within the genus, although all members cross-react serologically to some extent (7).…”

Section: Introductionmentioning

confidence: 99%

“…Serological studies have shown that although BVDV field isolates are antigenically related to each other, antigenic differences can be readily detected among viruses (7,(13)(14)(15). These antigenic differences have been demonstrated by the use of monoclonal antibodies (mAbs) and by in vitro and in vivo cross-neutralization studies (7,(13)(14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…These antigenic differences have been demonstrated by the use of monoclonal antibodies (mAbs) and by in vitro and in vivo cross-neutralization studies (7,(13)(14)(15)(16). However, in spite of the antigenic variability, no clear definition of serotypes has been possible to date (7,16). The marked antigenic variability of BVD viruses allows differentiation between strains, but also represents a potential problem for diagnosis and immunization strategies (7,16).…”

Section: Introductionmentioning

confidence: 99%

“…However, in spite of the antigenic variability, no clear definition of serotypes has been possible to date (7,16). The marked antigenic variability of BVD viruses allows differentiation between strains, but also represents a potential problem for diagnosis and immunization strategies (7,16).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Antigenic characterization of Brazilian bovine viral diarrhea virus isolates by monoclonal antibodies and cross-neutralization

Botton

DaSilva

Brum

et al. 1998

Braz J Med Biol Res

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Nineteen Brazilian isolates of bovine viral diarrhea virus (BVDV) were characterized antigenically with a panel of 19 monoclonal antibodies (mAbs) (Corapi WV, Donis RO and Dubovi EJ (1990) American Journal of Veterinary Research, 55: 1388-1394. Eight isolates were further characterized by cross-neutralization using sheep monospecific antisera. Analysis of mAb binding to viral antigens by indirect immunofluorescence revealed distinct patterns of reactivity among the native viruses. Local isolates differed from the prototype Singer strain in recognition by up to 14 mAbs. Only two mAbs -one to the non-structural protein NS23/p125 and another to the envelope glycoprotein E0/gp48 -recognized 100% of the isolates. No isolate was recognized by more than 14 mAbs and twelve viruses reacted with 10 or less mAbs. mAbs to the major envelope glycoprotein E2/gp53 revealed a particularly high degree of antigenic variability in this glycoprotein. Nine isolates (47.3%) reacted with three or less of 10 E2/ gp53 mAbs, and one isolate was not recognized by any of these mAbs. Virus-specific antisera to eight isolates plus three standard BVDV strains raised in lambs had virus-neutralizing titers ranging from 400 to 3200 against the homologous virus. Nonetheless, many antisera showed significantly reduced neutralizing activity when tested against heterologous viruses. Up to 128-fold differences in cross-neutralization titers were observed for some pairs of viruses. When the coefficient of antigenic similarity (R) was calculated, 49 of 66 comparisons (74.24%) between viruses resulted in R values that antigenically distinguish strains. Moreover, one isolate had R values suggesting that it belongs to a distinct serologic group. The marked antigenic diversity observed among Brazilian BVDV isolates should be considered when planning diagnostic and immunization strategies.

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Section: Introductionmentioning

confidence: 99%