Antigenic relationship of West Nile strains by titre ratios calculated from cross-neutralization test results

Blackburn, N. K.; Thompson, Danielle; Jupp, P. G.

doi:10.1017/s0950268800068059

Cited by 15 publications

(8 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We observed variation in neutralization sensitivity that did not define the difference between lineage I and II viruses. Variation in neutralization sensitivity is consistent with previous observations for WNV and Yellow fever virus (Hammam et al, 1965;Peiris et al, 1982;Blackburn et al, 1987). In PRNT 90 tests, WN-DAK (22?7 %) was significantly more sensitive to neutralization by bird sera than either WN-NY (2?6 %) or WN-Is (5?0 %).…”

Section: Discussionsupporting

confidence: 90%

Serological evidence of West Nile virus, Usutu virus and Sindbis virus infection of birds in the UK

Buckley

Dawson

Moss

et al. 2003

Journal of General Virology

197

155

View full text Add to dashboard Cite

The introduction and rapid dispersal of the African flavivirus West Nile virus (WNV) throughout North America, and the high fatality rate due to encephalitis in birds, horses, other wildlife species and humans, has attracted major attention worldwide. Usutu virus, another flavivirus, came to prominence in 2001, when it was identified as the agent responsible for a drop in the bird population in Austria; previously this encephalitic virus was found only in birds and mosquitoes in Africa. Sindbis virus, a pathogenic alphavirus that causes arthritis, is widespread throughout Africa, Europe, Asia and Australia, infecting a range of arthropods and vertebrates and is genetically related to encephalitic viruses in North America. Currently there is no evidence that any of these viruses cause disease in the UK. Here the presence of virus-specific neutralizing antibodies is reported in the sera of resident and migrant birds in the UK, implying that each of these viruses is being introduced to UK birds, possibly by mosquitoes. This is supported by nucleotide sequencing that identified three slightly different sequences of WNV RNA in tissues of magpies and a blackbird. The detection of specific neutralizing antibodies to WNV in birds provides a plausible explanation for the lack of evidence of a decrease in the bird population in the UK compared with North America. The potential health risk posed to humans and animals by these viruses circulating in the UK is discussed.

show abstract

Section: Discussionsupporting

confidence: 90%

Serological evidence of West Nile virus, Usutu virus and Sindbis virus infection of birds in the UK

Buckley

Dawson

Moss

et al. 2003

Journal of General Virology

197

155

View full text Add to dashboard Cite

show abstract

“…Strain differences between St. Louis encephalitis viruses isolated from various geographical areas have also been detected [32]. On the basis of the MAb reactivities with the WN isolates from the Rondebult bird sanctuary four distinct strains can be recognized, supporting the findings of Blackburn et al [1]. While some isolates appeared to be the same strain as the H442 virus, others lacked the H442 strainspecific determinant defined by one of the MAbs (2D 12).…”

Section: Discussionsupporting

confidence: 70%

“…Strains from Africa, Europe, the U.S.S.R. and Middle East formed the Palearctic antigenic group serologically distinct from strains isolated in India [10,15]. Antigenic variation has, however, been shown to occur between West Nile (WN) strains isolated within a single region [1,27,28,39].…”

Section: Introductionmentioning

confidence: 99%

Antigenic analysis of West Nile virus strains using monoclonal antibodies

Besselaar

Blackburn

1988

Archives of Virology

Self Cite

View full text Add to dashboard Cite

Seventeen monoclonal antibodies (MAbs) were prepared against the flavivirus West Nile strain H442. While the majority of these were specific for the major envelope protein, MAbs directed against the NS1 and ns4a nonstructural proteins were also identified. The MAbs were tested by indirect immunofluorescence against 16 southern African West Nile (WN) isolates, representative strains from the two main WN antigenic groups and several viruses from other flavivirus complexes. The MAb reactivities ranged from WN strain-specific to broadly flavivirus-group reactive. Comparison of the local isolates revealed the presence of several different strains, all of which were antigenically distinct from the representative strains of the two WN antigenic groups.

show abstract

“…Studies have demonstrated that previous infection with heterologous flaviviruses reduces the incidence of encephalitis and can provide protection from fatal WNV challenge in a hamster model for WNV pathogenesis ( 20 , 21 ). In contrast, a neutralization study performed with WNV strains of different lineages demonstrated that neutralizing antibodies against an Indian WNV strain provided poor protection against a South African WNV strain ( 22 ). Our results demonstrated that prior immunization with KUN virus can provide protection from lethal NY99 challenge in crows.…”

Section: Discussionmentioning

confidence: 99%

Differential Virulence of West Nile Strains for American Crows

Brault

Langevin

Bowen

et al. 2004

Emerg. Infect. Dis.

184

169

View full text Add to dashboard Cite

Antigenic relationship of West Nile strains by titre ratios calculated from cross-neutralization test results

Abstract: SUMMARYThe antigenic relationship of ten South African West Nile isolates, the South African prototype virus H442, the Egyptian strain EGlO1 and the Indian strain G2266 were compared using titre ratios.

Cited by 15 publications

References 9 publications

Serological evidence of West Nile virus, Usutu virus and Sindbis virus infection of birds in the UK

Serological evidence of West Nile virus, Usutu virus and Sindbis virus infection of birds in the UK

Antigenic analysis of West Nile virus strains using monoclonal antibodies

Differential Virulence of West Nile Strains for American Crows

Contact Info

Product

Resources

About