Antigenicity of structural components from porcine transmissible gastroenteritis virus

Abstract

Garwes, D.J., Lucas, M.H., Higgins, D.A., Pike, B.V. and Cartwright, S.F., 1978/1979. Antigenicity of structural components from porcine transmissible gastroenteritis virus. Vet. Microbiol., Pregnant sows were inoculated with inactivated transmissible gastroenteritis virus and with preparations of virus surface projections and subviral particles derived by detergent treatment of the virus. Neutralising antibody was demonstrated in serum and colostrum from animals that received whole virus or preparations of surface projections whereas subviral particles failed to stimulate neutralising antibody formation. Similar results were obtained with serum from rabbits inoculated with whole virus and structural components. All three preparations stimulated the formation of agglutinating antibodies, as demonstrated by sedimentation analysis and filtration studies with radiolabelled virus.The immunoglobulin classes responsible for neutralising antibody activity in sows inoculated by the intramarnmary route were examined. In each case where the immunoglobulin class was determined, IgG was found. One sow that received surface projections also had IgA with neutralising activity in her colostrum. In contrast, infection of sows with live whole virus resulted in neutralising antibody of the IgG, IgM and IgA classes.

Field Isolates of Transmissible Gastroenteritis Virus Differ at the Molecular Level from the Miller and Purdue Virulent and Attenuated Strains and from Porcine Respiratory Coronaviruses.

“…TGEV virions contain three major structural proteins: the spike (S) glycoprotein, the integral membrane (M) glycoprotein, and the nucleocapsid (N) protein, which are the translation products from mRNAs 2, 6, and 7, respectively. The S glycoprotein forms the peplomers on the virion envelop and induces neutralizing antibodies [9,10,14,20]. Unlike other coronaviruses, the S glycoprotein of TGEV, feline infectious peritonitis virus and canine coronavirus is not cleaved into two proteins [11,18,19].…”

Differences in antibody production against mouse hepatitis virus (MHV) among mouse strains

“…MHV-2 and MHV-NuU are considered to be of the same serotype but distinct from that of MHV·IHM by VN test (unpublished observation)_ FI hybrids of C57BL/6N mice, B6C3Fl and BDP1, showed CF antibody responses as high as those of C57BL/6N, suggesting that high reactivity oIC5 7BL/6N mice might be genetically determined. Differences in susceptibility to MHV infection among mouse strains have been studied by some Macnaughton, Hasony, Madge & Reed, 1981;Garwes, Lucas, Higgins, Pike & Cartwright, 1978). In this context it is noteworthy that sera from mice immunized with MHV-NuU failed to react with MHV-IHM antigen in the CF test, though they reacted not only with the homologous MHV-NuU but also with MHV·2 antigen.…”

cDNA Cloning and Sequence Analysis of the Gene Encoding the Peplomer Protein of Feline Infectious Peritonitis Virus

“…The peplomer protein mediates the binding of virions to the host cell receptor and is involved in membrane fusion (Sturman & Holmes, 1983). Moreover, in the case of MHV (Hasony & Macnaughton, 1981 ;Collins et al, 1982), IBV (Cavanagh et al, 1986b) and porcine transmissible gastroenteritis virus (TGEV) (Garwes et al, 1978), the E2 appears to be the major inducer of protective immunity. The role of the E2 protein 0000-7767 © 1987 SGM R. J.…”