Antilisterial active compound from lactic acid bacteria present on fresh iceberg lettuce

Abstract

Pediococcus pentosaceus DT016, a bacteriocin producing strain, was isolated from fresh lettuce. A protein with antilisterial activity (bacDT016), between 11 to 17 kDa, was identifi ed and characterized as the bioactive substance from the LAB culture. Highest bacteriocin production was noted after 15 h of growth. Antibacterial activity was affected by some enzymes and detergents, as well as by temperatures equal or above 80 ºC. DT016 strain contains an 1110 bp DNA fragment with homology to pediocin AcH/PA-1.