Antimicrobial activities and phylogenetic study of bacteria associated with Cyperus rotundus rhizosphere from Cemoro Sewu Plateau, Indonesia

Ambarwati, Ambarwati; Wahyuono, Subagus; Moeljopawiro, Sukarti; Sembiring, Langkah; Yuwono, Triwibowo

doi:10.13057/biodiv/d200814

Cited by 3 publications

(3 citation statements)

References 33 publications

(30 reference statements)

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“…Several studies have been conducted to find the antimicrobial substance from fungi, including: endophytes (Bhardwaj et al 2017) and bacteria (Nayaka et al 2019;Ambarwati et al 2019), endophyte bacteria (Leonita et al 2015;Yuwantiningsih et al 2015;Mohammed et al 2017;Syed et al 2017;Gislin et al 2018;Moi et al 2018), Actinomycetes (Wulandari and Rahayu. 2015;Retnowati et al 2017;Nayaka et al 2018;Al-Ansari et al 2019), and Streptomyces (Beiranvand et al 2017;Ambarwati et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of Streptomyces producing antimicrobial agent isolated from Kukup Beach Sand, Yogyakarta, Indonesia

2023

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Abstract. Ambarwati A, Santoso B, Sofyan A. 2023. Phylogenetic analysis of Streptomyces producing antimicrobial agent isolated from Kukup Beach Sand, Yogyakarta, Indonesia. Biodiversitas 24: 2374-2383. Microorganisms such as bacteria, fungi, and Streptomyces are sources of bioactive compounds. Streptomyces is known as the largest antibiotic-producing genus. This study aimed to determine the antimicrobial activity of Streptomyces isolates and analyze the relationship between Streptomyces isolates based on the 16S rRNA gene sequence. The Streptomyces isolates were screened for their antimicrobial activity based on their ability to inhibit test bacteria growth using the agar block method. The selected isolates that showed antimicrobial activity were molecularly characterized using 16S rRNA gene sequence analysis. The result showed 32 Streptomyces isolates successfully isolated on SCA and Raffinosa Histidine Agar media. Among 32 isolates, it was observed that five isolates demonstrated the ability to inhibit the test bacteria growth on 9 to 25 mm of inhibition zone diameters. The BRI-18 isolate showed the highest antimicrobial activity inhibiting Bacillus subtilis G. FNCC 0060 on 25 mm of inhibition zone diameter (strong inhibition category). Based on 16S rRNA sequences, it was known that five isolates belonged to Streptomyces. BLAST analysis on the ARA-5 isolate revealed that it was closely related to Streptomyces griseoincarnatus (Preobrazhenskaya et al. 1957) Pridham et al. 1958 strain JCM 4381 with 99.62% sequence similarities. The AR3-29 isolate was a sister clade identical to Streptomyces rochei Berger et al. 1953 strain NRRL B-1559 with a 99.35% similarity level. The BRI-18 and BRI-19 isolates were the most similar to Streptomyces fradiae (Waksman & Curtis) Waksman & Henrici 1948 strain NBRC 12773 on 96.87% and 95.15% of index similarity, respectively, whereas the BRI-35 isolate demonstrated the highest similarity to Streptomyces zhihengii Huang et al. 2017 strain YIM T102 (97.02%). The research demonstrated that Streptomyces isolates from Kukup beach sand showed the potential as an antimicrobial agent.

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Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of Streptomyces producing antimicrobial agent isolated from Kukup Beach Sand, Yogyakarta, Indonesia

2023

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“…Several species in this family are used in folk medicine (Hibi et al 2019). The studies stated that the extract of the Cyperus rotundus root had an analgesic effect on mice, and its rhizosphere contains bacteria with antimicrobial abilities (Ambarwati et al 2019;Puspitasari et al 2003). Cyperus malaccensis, which has become a vital fiber crop and grown in Japan and China, is the raw material for making mats (Shioya et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Morphology, biology, and damage behaviors of nutsedge borer (Bactra venosana Zeller) in Tra Vinh Province, Vietnam

et al. 2022

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Abstract. Nguyen HU, Phan QN, Dang HT, Son TTN, Nguyen HN, Nguyen THT. 2021. Morphology, biology, and damage behaviors of nutsedge borer (Bactra venosana Zeller) in Tra Vinh Province, Vietnam. Biodiversitas 23: 733-741. Nutsedge borer, Bactra venosana Zeller (Lepidoptera: Tortricidae), is a common pest of sedge in Tra Vinh Province. To control this species, its morphology, biology, and behaviors were investigated in Tra Vinh Province. The study was conducted with the aim of determining the information related to the Bactra venosana, as a basis for developing a strategy to manage this species by means of safety solutions. The study was carried out by surveying the behavior of nutsedge borer in sedge fields and determining their morphological and biological characteristics under laboratory conditions. The results showed that the eggs were 1.06 ± 0.17 mm (in length), 0.84 ± 0.16 (in width), oval, and white after turning pale yellow. Larvae progressed through 5 instars with body length and width of 1.20 ± 0.18 mm to 11.3 ± 1.23 mm and from 0.25 ± 0.05 to 1.05 ± 0.25 mm, respectively. Newly hatched larvae were dark yellow, and pupae were pale green. The male pupa was 7.75 ± 0.68 mm long, while the female was 8.46 ± 0.93 mm long, light green, and dark brown about to mature. The male adult of B. venosana was usually gray-brown and 7.70 ± 0.60 mm in body length, whereas the female adult was silver-gray, 8.41 ± 0.86 mm. The life cycle passed 35.9 ± 2.55 days in laboratory conditions. Under field conditions, eggs were laid scattered on the surface of the host plants. Larvae bored inside the stems of sedge. Newly damaged sedge plants had no symptoms at first whose bodies got soft, turned from light green to pale yellow, and gradually wilted and died.

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“…Cultivated mainly for its tubers, tigernut is utilized to produce food (Adomou et al 2012), commercial products (Warouma 2013), medicinal materials (Ambarwati et al 2019), and so on. In Niger, the crop development had led to an increase in national production with an estimate up to 100000 tons (Warouma et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Folk classification and traditional uses of Cyperus esculentus, a neglected and underutilized species in Benin

et al. 2021

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Abstract. Adjahossou VN, Gbemavo DSJC, Abidja S, Laly J, Gbaguidi AA, Anagonou AD. 2021. Folk classification and traditional uses of Cyperus esculentus, a neglected and underutilized species in Benin. Biodiversitas 22: 2972-2979. The sweet pea or tigernut (Cyperus esculentus L.) has very high potential to be developed but is considered a neglected and underutilized plant in Benin. The current study aims to investigate the indigenous knowledge related to local diversity, production and uses of various landrace types of tigernut grown in its production areas in Benin. Using Participatory Research Appraisal (PRA) method, we surveyed 113 tigernut farmers in different regions of production in Atacora Department, Benin. Univariate statistics were performed to describe and compare tigernut farmers' responses on botanical and agronomic criteria. We found that the tigernut farmers in the studied area distinguished three landraces of C. esculentus. Two landrace types produced yellow-skinned tubers and the other produced black-skinned tubers. Economic value, agro-morphological traits and organoleptic quality were the criteria considered by the farmers when choosing the type of landrace to cultivate. According to the farmers, the attacks of the tubers by earthworms and termites constituted the main constraints of the production of tigernut. Local people used the species mainly for food, while small proportion was for medicine (e.g., for aphrodisiac) and soil fertilization purposes. This study implies that C. esculentus in Benin deserves better attention to be conserved and developed.

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Antimicrobial activities and phylogenetic study of bacteria associated with Cyperus rotundus rhizosphere from Cemoro Sewu Plateau, Indonesia

Cited by 3 publications

References 33 publications

Phylogenetic analysis of Streptomyces producing antimicrobial agent isolated from Kukup Beach Sand, Yogyakarta, Indonesia

Phylogenetic analysis of Streptomyces producing antimicrobial agent isolated from Kukup Beach Sand, Yogyakarta, Indonesia

Morphology, biology, and damage behaviors of nutsedge borer (Bactra venosana Zeller) in Tra Vinh Province, Vietnam

Folk classification and traditional uses of Cyperus esculentus, a neglected and underutilized species in Benin

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