Antimicrobial Activities and Time‐Kill Kinetics of Extracts of Selected Ghanaian Mushrooms

Appiah, Theresa; Boakye, Yaw Duah; Agyare, Christian

doi:10.1155/2017/4534350

Cited by 103 publications

(71 citation statements)

References 47 publications

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“…Moreover, Knezevic et al (2015) assessed the protective activity of T. gibbosa extracts against H 2 O 2 -induced DNA damage in human peripheral blood leukocytes. Finally, methanol extracts of T. gibbosa showed a broad spectrum of activity against a number of pathogens such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus etc (Appiah et al, 2017)…”

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confidence: 99%

Basidiomycota isolated from the Mediterranean Sea – Phylogeny and putative ecological roles

et al. 2018

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Basidiomycota are an ecologically and taxonomically diverse fungal phylum, colonizing all terrestrial ecosystems, with 30,000 described species. By contrast, in marine habitats Basidiomycota are underrepresented when compared to the more abundant Ascomycota. Recently, we investigated the marine mycobiota mainly in the Mediterranean Sea, confirming the scarcity of Basidiomycota. However, a low rank taxonomic identification based on morphological features, proved impossible, since most of the strains remained sterile in axenic culture. Nevertheless, considering the great potential and biotechnological value of Basidiomycota, it would be useful to define their precise taxonomic placement. To this end, 34 marine Basidiomycota isolated from different marine substrates underwent molecular analyses and 123 newly generated sequences were obtained and deposited in GenBank. Sequencing of the Internal Transcribed Spacer (nrITS) regions allowed us to affiliate the 34 strains with six classes, but a lower taxonomic identification was reached with a multi-locus phylogenetic analysis.

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confidence: 99%

Basidiomycota isolated from the Mediterranean Sea – Phylogeny and putative ecological roles

et al. 2018

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“…For fungal colony forming unit measurements, we followed a previously published protocol [70]. Briefly, 1 mL of untreated and Ar, Sc, and Cs-treated sample was pipetted at 0, 8, and 16 h time intervals and was diluted 10 5 times followed by spreading 50 µL onto 20 mL YPD agar medium using a cell spreader and incubated at 30 • C for 24 h. Cs was used as a reference control.…”

Section: Determination Of the Metabolic Activity With Resazurin Assaymentioning

confidence: 99%

Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species

et al. 2020

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We investigated the antifungal activities of purified plant metabolites artemisinin (Ar) and scopoletin (Sc) including inhibition, effects on metabolic activities, viability, and oxidative stress on planktonic forms and on preformed biofilms of seven Candida species. The characteristic minimum inhibitory concentration (MIC90) of Ar and Sc against Candida species ranged from 21.83–142.1 µg/mL and 67.22–119.4 µg/mL, respectively. Drug concentrations causing ≈10% CFU decrease within 60 min of treatments were also determined (minimum effective concentration, MEC10) using 100-fold higher CFUs than in the case of MIC90 studies. Cytotoxic effects on planktonic and on mature biofilms of Candida species at MEC10 concentrations were further evaluated with fluorescent live/dead discrimination techniques. Candida glabrata, Candida guilliermondii, and Candida parapsilosis were the species most sensitive to Ar and Sc. Ar and Sc were also found to promote the accumulation of intracellular reactive oxygen species (ROS) by increasing oxidative stress at their respective MEC10 concentrations against the tested planktonic Candida species. Ar and Sc possess dose-dependent antifungal action but the underlying mechanism type (fungistatic and fungicidal) is not clear yet. Our data suggest that Ar and Sc found in herbal plants might have potential usage in the fight against Candida biofilms.

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“…Microbial test of sample C against V. cholerae (microbial time-kill kinetic assay). Microbial time-kill kinetic assay was performed on V. cholerae O1 biotype according to the procedure described by Appiah et al, 2017, [27] with slight modifications. A 24 h broth culture of the test organism was used with organism count of 1.0 x 10 6 CFU/mL.…”

Section: Part 3 (Finished Product Analysis (Sample C))mentioning

confidence: 99%

Managing Vibrio cholerae with a local beverage: preparation of an affordable ethanol based hand sanitizer

Osei-Asare

Oppong

Apenteng

et al. 2020

Heliyon

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Research indicates an increased use of hand sanitizers globally, and in particular developing countries where bacterial epidemics such as cholera are common. While there is evidence of availability, high demand and use of hand sanitizers, the incidence of cholera in developing countries remains unabated. Several reasons have been put forward, and cost of hand sanitizers remains dominant. It is in response to this contradictory situation of availability but limited access, that this study was conducted to present an alternative option of formulating a high quality and cost effective ethanol-based sanitizer from a Ghanaian local beverage (akpeteshie). The concentration of ethanol in akpeteshie was determined using gas chromatography. An Ethanol based hand sanitizer (Sample C) was formulated with akpeteshie and tested against Vibrio cholerae using the microbial time-kill kinetics assay. Commercially available ethanol based Equi-Clean hand sanitizer (62%) (Sample D) was used as the standard. Results show that the akpeteshie contained 73.08% ethanol and formulated product (Sample C) contained 63.70% ethanol. Viscosity and pH of Sample C were; 89 rpm (1.48 cps) and 7.30 respectively whiles that of Sample D were; 80 rpm (1.33 cps) and 7.50 respectively. The formulated product (Sample C) was effective against Vibrio cholerae with a gradual reduction in microbial count upon exposure to the organisms at time intervals of 0, 5, 15, 30, 60 and 120 min.

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Antimicrobial Activities and Time‐Kill Kinetics of Extracts of Selected Ghanaian Mushrooms

Cited by 103 publications

References 47 publications

Basidiomycota isolated from the Mediterranean Sea – Phylogeny and putative ecological roles

Basidiomycota isolated from the Mediterranean Sea – Phylogeny and putative ecological roles

Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species

Managing Vibrio cholerae with a local beverage: preparation of an affordable ethanol based hand sanitizer

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