Antimicrobial Activity and Identification of the Biosynthetic Gene Cluster of X-14952B From Streptomyces sp. 135

Li, Na; Chen, Simin; Yan, Zhiqiang; Han, Jinhua; Ta, Yongquan; Pu, Taixun; Wang, Yonghong

doi:10.3389/fmicb.2021.703093

Cited by 9 publications

(6 citation statements)

References 51 publications

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“…Germicidin A, a pyranone autoregulator of sporulation [ 49 ], was putatively present in the extracts from N. alba (PMIC_1A11B.2 and PMIC_2A11B.1). The antibiotic X-14952B, a 20-membered macrolide lactone with broad-spectrum antibacterial properties [ 50 , 51 ], was found in the extracts of N. alba PMIC_2H2C.2 and PMIC_2A11B.1. Also present in the extract of N. alba PMIC_1F6A.3 was cyclo(Tyr-Leu), a diketopiperazine that exhibits antibacterial [ 52 ] and antifungal [ 53 ] activities.…”

Section: Resultsmentioning

confidence: 99%

iChip-Inspired Isolation, Bioactivities and Dereplication of Actinomycetota from Portuguese Beach Sediments

et al. 2022

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Oceans hold a stunning number of unique microorganisms, which remain unstudied by culture-dependent methods due to failures in establishing the right conditions for these organisms to grow. In this work, an isolation effort inspired by the iChip was performed using marine sediments from Memoria beach, Portugal. The isolates obtained were identified by 16S rRNA gene analysis, fingerprinted using BOX-PCR and ERIC-PCR, searched for the putative presence of secondary metabolism genes associated with polyketide synthase I (PKS-I) and non-ribosomal peptide synthetases (NRPS), screened for antimicrobial activity against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213, and had bioactive extracts dereplicated by LC/HRMS. Of the 158 isolated strains, 96 were affiliated with the phylum Actinomycetota, PKS-I and NRPS genes were detected in 53 actinomycetotal strains, and 11 proved to be bioactive (10 against E. coli, 1 against S. aureus and 1 against both pathogens). Further bioactivities were explored using an “one strain many compounds” approach, with six strains showing continued bioactivity and one showing a novel one. Extract dereplication showed the presence of several known bioactive molecules and potential novel ones in the bioactive extracts. These results indicate the use of the bacteria isolated here as sources of new bioactive natural products.

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Section: Resultsmentioning

confidence: 99%

iChip-Inspired Isolation, Bioactivities and Dereplication of Actinomycetota from Portuguese Beach Sediments

et al. 2022

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“…HSG: humic acid 0.1 g, CaCl 2 0.02 g, FeSO 4 ·7H 2 O 1 mg, MnCl 2 ·4H 2 O 1 mg, ZnSO 4 ·7H 2 O 1 mg, NiSO 4 ·6H 2 O 1 mg, CHES 5 mmol, gellan gum 7 g, agar 15 g, and pure water 1 l ( Li et al, 2021a ).…”

Section: Methodsmentioning

confidence: 99%

“…HV: humic acid 1 g, CaCO 3 0.02 g, Na 2 HPO 4 0.5 g, KCI 0.5 g, MgSO 4 ·7H 2 O 0.5 g, FeSO 4 ·7H 2 O 0.1 g, agar 15 g, and pure water 1 l ( Li et al, 2021a ).…”

Section: Methodsmentioning

confidence: 99%

Actinobacteria Community and Their Antibacterial and Cytotoxic Activity on the Weizhou and Xieyang Volcanic Islands in the Beibu Gulf of China

et al. 2022

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Weizhou Island and Xieyang Island are two large and young volcanic sea islands in the northern part of the South China Sea. In this study, high-throughput sequencing (HTS) of 16S rRNA genes was used to explore the diversity of Actinobacteria in the Weizhou and Xieyang Islands. Moreover, a traditional culture-dependent method was utilized to isolate Actinobacteria, and their antibacterial and cytotoxic activities were detected. The alpha diversity indices (ACE metric) of the overall bacterial communities for the larger island (Weizhou) were higher than those for the smaller island (Xieyang). A beta diversity analysis showed a more dispersive pattern of overall bacterial and actinobacterial communities on a larger island (Weizhou). At the order level, Frankiales, Propionibacteriales, Streptomycetales, Micrococcales, Pseudonocardiales, Micromonosporales, Glycomycetales, Corynebacteriales, and Streptosporangiales were the predominant Actinobacteria. A total of 22.7% of the OTUs shared 88%–95% similarity with some known groups. More interestingly, 15 OTUs formed a distinct and most predominant clade, and shared identities of less than 95% with any known families. This is the first report about this unknown group and their 16S rRNA sequences obtained from volcanic soils. A total of 268 actinobacterial strains were isolated by the culture-dependent method. Among them, 55 Streptomyces species were isolated, representing that 76.6% of the total. S. variabilis and S. flavogriseus were the most abundant. Moreover, some rare Actinobacteria were isolated. These included Micromonospora spp., Nocardia spp., Amycolatopsis spp., Tsukamurella spp., Mycobacterium spp., and Nonomuraea spp. Among them, eight Streptomyces spp. exhibited antibacterial activity against Bacillus cereus. Only three strains inhibited the growth of Escherichia coli. Four strains showed good activity against aquatic pathogenic bacterial strains of Streptococcus iniae. The cytotoxicity assay results showed that 27 strains (10.07%) exhibited cytotoxic activity against HeLa and A549 cell lines. Many actinobacterial strains with cytotoxic activity were identified as rare Actinobacteria, which illustrated that volcanic islands are vast reservoirs for Actinobacteria with promising antibacterial and cytotoxic activity. This study may significantly improve our understanding of actinobacterial communities on volcanic islands. The isolated Actinobacteria showed promising prospects for future use.

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“…Physiological characteristics such as temperature tolerance, NaCl tolerance, and pH level were investigated. Various enzymatic activity tests like catalase, oxidase, nitrate reductase, amylase, caseinase, gelatin hydrolysis citrate utilization, and melanin production tests were also performed according to the previous description in [ 9 , 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…Currently, the majority of the commercially and medically known antibiotics have originated from a different genus of actinomycetes [ 6 , 7 ]. Actinomycetes have also been the source of many industrially and clinically important bioactive metabolites in treating cancer, inflammatory diseases, allergies, anthelmintics, autoimmune diseases, infections, and others [ 8 , 9 ]. Among actinomycetes genera, Streptomyces are the largest genus and the most frequent producer of antibiotics which is continuously being explored for antimicrobial drug discovery [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Potential of Streptomyces spp. Isolated from the Rift Valley Regions of Ethiopia

Elias

Muddada

Muleta

et al. 2022

Advances in Pharmacological and Pharmaceutical Sciences

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The study was undertaken to isolate, screen, and identify actinomycetes with antimicrobial metabolites. Twenty-one composite soil samples were randomly collected from various unique agroecological niches in the Rift Valley of Ethiopia. The soil samples were serially diluted and spread on starch casein agar medium supplemented with 50 μg/ml cycloheximide and 25 μg/ml nalidixic acid. Two hundred and forty-nine (249) actinomycetes cultures were isolated and screened by cross streaking against various human pathogens. Twenty-four isolates with pronounced antimicrobial activity were selected for identification and further screening. Among the isolates, 172 (69.1%) showed antimicrobial activities against tested pathogens. The inhibition zone of the isolates ranged from 5 ± 0.31 to >40 mm during primary screening. The antimicrobial activity of the crude extracts of promising isolates showed a statistically significant difference P < 0.05 between them and the control. The isolates RVE129 and RVE217 showed the maximum zone of inhibition at 27 ± 0.6 mm and 26 ± 0.6 mm, respectively, against S. aureus, and the results were higher than the standard drug streptomycin (25 ± 0.58 mm). The inhibition zone of crude extracts from RVE129 was at the maximum of 22 ± 0.0 mm against P . aeruginosa, almost comparable to the standard drug streptomycin (24 ± 0.58 mm). Crude extract from the isolates RVE129 and RVE187 showed higher inhibition zones of 22 ± 0.6 mm and 16 ± 0.33 mm against A. niger ATCC10535, which, however, were smaller than those obtained with the standard drug amphotericin B (29 ± 0.6 mm). Twenty-four actinomycete strains with remarkable bioactivity were characterized using various cultural, morphological, physiological, and biochemical characteristics and assigned under the genus Streptomycetes. The finding of the current study indicates that Streptomyces sp. isolated from the Rift Valley of Ethiopia was found to possess a broad spectrum of bioactivity against a range of human pathogens.

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Antimicrobial Activity and Identification of the Biosynthetic Gene Cluster of X-14952B From Streptomyces sp. 135

Cited by 9 publications

References 51 publications

iChip-Inspired Isolation, Bioactivities and Dereplication of Actinomycetota from Portuguese Beach Sediments

iChip-Inspired Isolation, Bioactivities and Dereplication of Actinomycetota from Portuguese Beach Sediments

Actinobacteria Community and Their Antibacterial and Cytotoxic Activity on the Weizhou and Xieyang Volcanic Islands in the Beibu Gulf of China

Antimicrobial Potential of Streptomyces spp. Isolated from the Rift Valley Regions of Ethiopia

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