Antimicrobial activity of copper surfaces against suspensions of Salmonella enterica and Campylobacter jejuni

Faundez, Gustavo; Troncoso, Miriam; Navarrete, Paola; Figueroa, Guillermo

doi:10.1186/1471-2180-4-19

Cited by 240 publications

(78 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Living organisms need Cu 2+ at low concentrations as cofactors for metalloproteins and enzymes; however, at high concentrations, Cu 2+ induces an inhibition of growth in bacteria (Gordon et al, 1994). Many researchers have suggested that Cu 2+ has antibacterial activity against harmful bacteria (Faundez et al, 2004;Hu and Xia, 2006;Kanhed et al, 2014). The exchange of montmorillonite with Cu 2+ enhances the antibacterial activity and leads to improved growth performance and a decreased incidence of diarrhea in pigs (Hu and Xia, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…In particular, these ions can induce the inhibition of bacterial growth and have toxic effects on most microorganisms. Copper has seen widespread use in the preparation of antibacterial materials (Faundez et al, 2004;Kanhed et al, 2014). Copperexchanged montmorillonite has antibacterial activity against Escherichia coli K88, and leads to improved growth performance and a decreased incidence of diarrhea in pigs Zhou et al, 2004;Hu and Xia, 2006).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Copper-modified palygorskite is effective in preventing and treating diarrhea caused by Salmonella typhimurium

Yao

et al. 2017

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Abstract:The aim of this research was to develop effective alternative therapies to reduce antibiotic use in animal agriculture. In this study, the efficacy of copper-modified palygorskite (CM-Pal) in preventing diarrhea caused by Salmonella was specifically examined both in vitro and in vivo. The CM-Pal was prepared with palygorskite (Pal) and copper nitrate. The antibacterial activity of the CM-Pal was detected by comparing the differences in cell numbers on plate count agar before and after adding the CM-Pal to Salmonella typhimurium cultures. Seventy ICR mice were then allocated into seven groups. Five groups (the treatment groups) were infected with S. typhimurium by intraperitoneal (i.p.) injection and were given Pal, CM-Pal, montmorillonite powder, gentamicin, and physiological saline, respectively. One group (the prevention group) was given CM-Pal before infection with S. typhimurium. Another group (the uninfected group) was not infected with S. typhimurium. The effects of Pal, CM-Pal, montmorillonite powder, and gentamicin on the treatment or prevention of diarrhea in the mice were examined by stool studies, fecal scoring, and assessment of growth performance and villus height. The CM-Pal had satisfactory anti-bacterial properties in vitro: the antibacterial rate was 100% after 2 h incubation with S. typhimurium NJS1 cultures (1×10 6 colony-forming units (CFU)/ml). In the in vivo experiment, the CM-Pal exerted superior effects in the treatment and prevention of diarrhea in mice compared with Pal, montmorillonite powder, and gentamicin. In the CM-Pal group, no mice showed signs of diarrhea at 24 h post infection (p.i.), and all mice fully recovered from infection. However, the Pal group, montmorillonite group, and gentamicin group only recovered after 48, 48, and 96 h, respectively. The villus height level in the CM-Pal treatment group recovered at 3 d p.i. However, the recovery time of the other groups was longer (at least 5 d). The CM-Pal prevention group had a better effect on weight gain than the other groups. This study suggested that CM-Pal may be an effective alternative to conventional antibiotics for the treatment and prevention of animal diarrhea caused by Salmonella.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Copper-modified palygorskite is effective in preventing and treating diarrhea caused by Salmonella typhimurium

Yao

et al. 2017

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

show abstract

“…In the last study, the use of copper in metallic state (Cu 0 ) was studied, because it is a highly reactive species that can produce all antimicrobial species listed above, thereby causing bacterial death 33,36 . Copper antimicrobial activity has been confirmed over a broad spectrum of microorganisms such as Escherichia coli O157:H7, Staphylococcus aureus, Enterobacter aerogenes, Pseudomona aeruginosa 29 , Listeria monocytogenes 37 , Salmonella entérica, Campylobacter jejuni 38 , Klebsiella pneumoniae, Mycobacterium tuberculosis, Candida albicans 39 , Clostridium difficile 40 , Aspergillus flavus, Aspergillus niger, Penicillium chrysogenum 41 Saccharomyces cereviciae 42 .…”

mentioning

confidence: 99%

DEVELOPMENT OF ANTIBACTERIAL MtCu/PLA NANOCOMPOSITES BY CASTING METHOD FOR POTENTIAL USE IN FOOD PACKAGING

Bruna

Quilodrán

Guarda

et al. 2015

J. Chil. Chem. Soc.

View full text Add to dashboard Cite

Polylactic acid (PLA) nanocomposites with two antimicrobial agents based on copper modified montmorillonite (MtCu 2+ and MtCu 0 ) were developed in order to obtain a lower environmental impact material with antimicrobial activity for potential use in food packaging. Clay modification was permorfed by cation exchange between MtNa + and a copper salt obtaining MtCu 2+ and its followed reduction with NaBH 4 obtaining MtCu 0 . Then PLA nanocomposite films (MtCu 2+ / PLA and MtCu 0 /PLA), using different concentration of MtCu 2+ and MtCu 0 (1, 3 and 5 wt.%) were obtained by casting technique. X-ray diffraction (XRD) and Transmission electronic microscopy (TEM) analysis evidenced a certain degree of intercalation of the modified clays in the nanocomposites. Thermal, mechanical and optical properties showed variations by incorporating MtCu 2+ and MtCu 0 in the polymer matrix. On the other hand, it was possible to evidence antimicrobial activity of the nanocomposites (ASTM Standard E2149) against to Escherichia coli ATCC 25922 and Listeria innocua ATCC 33090 obtaining a 99% maximum of reduction to both bacteria.

show abstract

“…However, the solution phase technique is cheaper, and it does not require any organic compound or surfactant or a high temperature for Cu 2 O-NP synthesis (20). Chemically and physically, Cu 2 ONPs can be prepared with extremely high surface areas and unusual crystal morphologies, which act predominantly as valuable antistaphylococcal agents (2). Previously, it was observed that copper (Cu)-containing nanoparticles (Cu metal, Cu, CuO) caused cell membrane damage, depending on both the chemical composition (metal versus oxide) and surface area (21).…”

mentioning

confidence: 99%

“…Biofilms can be associated with a variety of complications, the worst being the risk of bacterial and fungal infections in surgical implanted devices. Bacteria embedded in biofilms are hard to eradicate with standard antibiotics and are intrinsically resistant to host immune responses (2). S. aureus strains with reduced susceptibility to vancomycin, such as heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) and vancomycin-intermediate Staphylococcus aureus (VISA), are being reported increasingly worldwide (3).…”

mentioning

confidence: 99%

Antibiofilm and Membrane-Damaging Potential of Cuprous Oxide Nanoparticles against Staphylococcus aureus with Reduced Susceptibility to Vancomycin

Singh

Ahmed

Prasad

et al. 2015

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The antimicrobial effects of copper ions and salts are well known, but the effects of cuprous oxide nanoparticles (Cu 2 O-NPs) on staphylococcal biofilms have not yet been clearly revealed. The present study evaluated Cu 2 O-NPs for their antibacterial and antibiofilm activities against heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) and vancomycin-intermediate S. aureus (VISA). Nanoscaled Cu 2 O, generated by solution phase technology, contained Cu 2 O octahedral nanoparticles. Field emission electron microscopy demonstrated particles with sizes ranging from 100 to 150 nm. Cu 2 O-NPs inhibited the growth of S. aureus and showed antibiofilm activity. The MICs and minimum biofilm inhibitory concentrations ranged from 625 g/ml to 5,000 g/ml and from 2,500 g/ml to 10,000 g/ml, respectively. Exposure of S. aureus to Cu 2 O-NPs caused leakage of the cellular constituents and increased uptake of ethidium bromide and propidium iodide. Exposure also caused a significant reduction in the overall vancomycin-BODIPY (dipyrromethene boron difluoride [4,4-difluoro-4-bora-3a,4a-diaza-s-indacene] fluorescent dye) binding and a decrease in the viable cell count in the presence of 7.5% sodium chloride. Cu 2 O-NP toxicity assessment by hemolysis assay showed no cytotoxicity at 625 to 10,000 g/ml concentrations. The results suggest that Cu 2 O-NPs exert their action by disruption of the bacterial cell membrane and can be used as effective antistaphylococcal and antibiofilm agents in diverse medical devices. Biofilm formation, one of the defense mechanisms of Staphylococcus aureus, represents a structural community of bacterial cells embedded in a self-produced polymeric matrix adherent to an artificial surface (1). Biofilms can be associated with a variety of complications, the worst being the risk of bacterial and fungal infections in surgical implanted devices. Bacteria embedded in biofilms are hard to eradicate with standard antibiotics and are intrinsically resistant to host immune responses (2). S. aureus strains with reduced susceptibility to vancomycin, such as heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) and vancomycin-intermediate Staphylococcus aureus (VISA), are being reported increasingly worldwide (3). The emergence of such strains is attributed to excess or irrational use of vancomycin and poor tissue penetration (4). Biofilm formation also plays an important role in the pathogenesis of staphylococcal infections, especially with prosthetic materials (5). It is presumed to be a significant initial step in the pathway to development of vancomycin resistance (6, 7). Biofilm infections are difficult to treat due to their inherent antibiotic resistance (8). Only limited numbers of antibiotics, such as daptomycin, quinupristin-dalfopristin, linezolid, and tigecycline, are active against the vancomycin-nonsusceptible S. aureus strains (9). Interestingly, daptomycin nonsusceptibility is also being reported for some hVISA and VISA isolates (10, 11). Despite antimicrobial therapy, the ...

show abstract

Antimicrobial activity of copper surfaces against suspensions of Salmonella enterica and Campylobacter jejuni

Cited by 240 publications

References 10 publications

Copper-modified palygorskite is effective in preventing and treating diarrhea caused by Salmonella typhimurium

Copper-modified palygorskite is effective in preventing and treating diarrhea caused by Salmonella typhimurium

DEVELOPMENT OF ANTIBACTERIAL MtCu/PLA NANOCOMPOSITES BY CASTING METHOD FOR POTENTIAL USE IN FOOD PACKAGING

Antibiofilm and Membrane-Damaging Potential of Cuprous Oxide Nanoparticles against Staphylococcus aureus with Reduced Susceptibility to Vancomycin

Contact Info

Product

Resources

About