Antimicrobial Activity of Novel Synthetic Peptides Derived from Indolicidin and Ranalexin against Streptococcus pneumoniae

Jindal, Hassan Mahmood; Le, Cheng Foh; Yusof, Mohd Yasim Mohd; Velayuthan, Rukumani Devi; Lee, Vannajan Sanghiran; Zain, Sharifuddin M.; Isa, Diyana Mohd; Sekaran, Shamala Devi

doi:10.1371/journal.pone.0128532

Cited by 72 publications

(64 citation statements)

References 66 publications

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“…The HC 50 /MIC values of linear α-polylysine and all the glycopolymer-polypeptide conjugates are generally higher than those of many other antimicrobial peptides, such as indolicidin, 43 8, 8, 5 and 3, respectively, when calculated using MIC toward S. aureus. The hemoselectivity of P(Lys) and P(GlyEMA) 10 -P(Lys) is even higher than that of polymyxin B, a topical antibiotic with an HC 50 /MIC value of approximately 141 against S. aureus.…”

Section: Antimicrobial Properties Of the Four-arm Star Glycopolymer-pmentioning

confidence: 91%

Increasing bacterial affinity and cytocompatibility with four-arm star glycopolymers and antimicrobial α-polylysine

Pranantyo

Zheng

et al. 2017

Polym. Chem.

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A series of four-arm star copolymers, incorporating glycopolymer and antimicrobial polypeptide domains, was developed in the design of forthcoming anti-infective agents. Mannose, glucose, and galactosebased glycopolymers with a variety of well-defined chain lengths were prepared via atom transfer radical polymerization, whereas linear α-polylysine was prepared via ring-opening polymerization of N-carboxyanhydride monomers. Copper-catalyzed azide-alkyne cycloaddition was employed for 'click' conjugation of the glycopolymer arms and the polypeptide chains. The glycopolymer-polypeptide conjugates were non-hemolytic and exhibited higher cytocompatibility than the linear α-polylysine. The conjugates with shorter chains of mannose-based glycopolymer arms showed an enhanced bactericidal efficacy against Gram-negative and Gram-positive bacteria, with a therapeutic selectivity half of that of the linear α-polylysine. The pendant mannose moieties of the conjugates increased microbial targeting due to their specific affinity for bacterial surfaces, and binding competition with free mannopyranoside was demonstrated. Therefore, the molecular combination of glycopolymers and polypeptides without loss of their respective activities provides an interesting concept in the design of antimicrobial agents to combat infectious disease.

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Section: Antimicrobial Properties Of the Four-arm Star Glycopolymer-pmentioning

confidence: 91%

Increasing bacterial affinity and cytocompatibility with four-arm star glycopolymers and antimicrobial α-polylysine

Pranantyo

Zheng

et al. 2017

Polym. Chem.

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“…Although higher net charge, hydrophobicity, helical propensity, and presence of a conserved sequence are able to enhance the antimicrobial activity of AMPs in general, however, these sets of rules do not apply equally to all classes of AMPs. For example, our previous studies showed that the antibacterial activity of five designed AMPs (DM1 to DM5) was correlated to net charge within specific window values (79,174,175). Anionic AMPs have also been documented in the literature (176,177).…”

Section: Important Remarksmentioning

confidence: 95%

Intracellular Targeting Mechanisms by Antimicrobial Peptides

Fang

Sekaran

2017

Antimicrob Agents Chemother

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431

366

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Antimicrobial peptides (AMPs) are expressed in various living organisms as first-line host defenses against potential harmful encounters in their surroundings. AMPs are short polycationic peptides exhibiting various antimicrobial activities. The principal antibacterial activity is attributed to the membrane-lytic mechanism which directly interferes with the integrity of the bacterial cell membrane and cell wall. In addition, a number of AMPs form a transmembrane channel in the membrane by self-aggregation or polymerization, leading to cytoplasm leakage and cell death. However, an increasing body of evidence has demonstrated that AMPs are able to exert intracellular inhibitory activities as the primary or supportive mechanisms to achieve efficient killing. In this review, we focus on the major intracellular targeting activities reported in AMPs, which include nucleic acids and protein biosynthesis and protein-folding, protease, cell division, cell wall biosynthesis, and lipopolysaccharide inhibition. These multifunctional AMPs could serve as the potential lead peptides for the future development of novel antibacterial agents with improved therapeutic profiles.

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“…60 After structures optimization, molecular docking was performed using AutoDock Vina, which is widely used in the interaction analysis of proteins with small molecules. 61,62 The Vina scoring function has the advantage of using the knowledge and empirical functions, extracting information about conformational preferences and the affinity of the receptor-ligand complex based on empirical data. 63 The program generates a maximum of 20 conformations for each complex.…”

Section: Resultsmentioning

confidence: 99%

Epitopes rationally selected through computational analyses induce T‐cell proliferation in mice and are recognized by serum from individuals infected with Schistosoma mansoni

Lopes

Oliveira

Coelho

et al. 2017

Biotechnology Progress

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Schistosomiasis is the second leading cause of death due to parasitic diseases in the world. Seeking an alternative for the control of disease, the World Health Organization funded the genome sequencing of the major species related to schistosomiasis to identify potential vaccines and therapeutic targets. Therefore, the aim of this work was to select T and B-cell epitopes from Schistosoma mansoni through computational analyses and evaluate the immunological potential of epitopes in vitro. Extracellular regions of membrane proteins from the Schistosoma mansoni were used to predict promiscuous epitopes with affinity to different human Major Histocompatibility Class II (MHCII) molecules by bioinformatics analysis. The three-dimensional structure of selected epitopes was constructed and used in molecular docking to verify the interaction with murine MHCII H2-IA . In this process, four epitopes were selected and synthesized to assess their ability to stimulate proliferation of CD4 T lymphocytes in mice splenocyte cultures. The results showed that Sm041370 and Sm168240 epitopes induced significant cell proliferation. Additionally, the four epitopes were used as antigens in the Indirect Enzyme-Linked Immunosorbent Assay (ELISA) to assess the recognition by serum from individuals infected with Schistosoma mansoni. Sm140560, Sm168240, and Sm041370 epitopes were recognized by infected individuals IgG antibodies. Therefore, Sm041370 and Sm168240 epitopes that stood out in in silico and in vitro analyses could be promising antigens in schistosomiasis vaccine development or diagnostic kits. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:804-814, 2017.

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Antimicrobial Activity of Novel Synthetic Peptides Derived from Indolicidin and Ranalexin against Streptococcus pneumoniae

Cited by 72 publications

References 66 publications

Increasing bacterial affinity and cytocompatibility with four-arm star glycopolymers and antimicrobial α-polylysine

Increasing bacterial affinity and cytocompatibility with four-arm star glycopolymers and antimicrobial α-polylysine

Intracellular Targeting Mechanisms by Antimicrobial Peptides

Epitopes rationally selected through computational analyses induce T‐cell proliferation in mice and are recognized by serum from individuals infected with Schistosoma mansoni

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