Antimicrobial Activity of Potato Rhizospheric Pseudomonas chlororaphis subsp. aureofaciens from Sétif Algeria

Mezaache-Aichour, Samia; Haichour, Nora; Nicklin, Jane; Zerroug, M. M.

doi:10.9734/arrb/2016/31651

Cited by 4 publications

(4 citation statements)

References 16 publications

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“…confers significant plasticity on these bacteria in their adaptation to various environments, such as the rhizosphere. Mezaache-Aichour et al (2012) obtained similar results where a population of P. fluorescens was elevated in the rhizosphere compared to other species.…”

Section: Discussionsupporting

confidence: 60%

Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blight

2023

Journal of Plant Protection Research

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Taro leaf blight caused by Phytophthora colocasiae affects plant health and is a major threat to taro culture in Cameroon. Chemical fertilizers used often harm the ecosystem. Plant growth-promoting rhizobacteria (PGPR) are better alternatives that increase plant growth promotion and suppress phytopathogens. In the present study, a total of 67 fluorescent Pseudomonas spp. was characterized by 17.91, 5.97, and 4.47% populations of P. fluorescens, P. chlororaphis, and P. putida, respectively, among the most represented. More than 36% of bacteria showed antagonistic potential through the production of both diffusible and volatile compounds. Some of them (03) exhibited antagonistic activity in dual culture against P. colocasiae with a diameter greater than 13 mm. These rhizobacteria produced a significant amount of siderophore, IAA, SA, HCN, protease, lipases, and cellulases. For the pot experiment, treatment by Pseudomonas significantly increased the enzymatic activity involved in the resistance of taro, such as peroxidase (PO), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL). The two antagonists also increased plant growth parameters of taro such as chlorophyll, plant height, shoot length, total leaf surface, fresh root biomass, and fresh leaf biomass. These findings showed that fluorescent Pseudomonas have an intriguing and undeniable potential in the fight against P. colocasiae, which could lead to the development of a biopesticide in the future.

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Section: Discussionsupporting

confidence: 60%

Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blight

2023

Journal of Plant Protection Research

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“…bacteria have also been isolated in studies on their insecticidal activity [40]. To date, the ability of P. chlororaphis to inhibit the growth of Photorhabdus or Xenorhabdus bacteria has not been shown, but its antibacterial activity against other species, e.g., Clavibacter michiganensis [41], Bacillus subtilis, and Salmonella enteritidis [42], or Staphylococcus aureus [43], has been reported.…”

Section: Discussionmentioning

confidence: 99%

Bacteria from the Midgut of Common Cockchafer (Melolontha melolontha L.) Larvae Exhibiting Antagonistic Activity Against Bacterial Symbionts of Entomopathogenic Nematodes: Isolation and Molecular Identification

Skowronek

Sajnaga

Pleszczyńska

et al. 2020

IJMS

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The mechanisms of action of the complex including entomopathogenic nematodes of the genera Steinernema and Heterorhabditis and their mutualistic partners, i.e., bacteria Xenorhabdus and Photorhabdus, have been well explained, and the nematodes have been commercialized as biological control agents against many soil insect pests. However, little is known regarding the nature of the relationships between these bacteria and the gut microbiota of infected insects. In the present study, 900 bacterial isolates that were obtained from the midgut samples of Melolontha melolontha larvae were screened for their antagonistic activity against the selected species of the genera Xenorhabdus and Photorhabdus. Twelve strains exhibited significant antibacterial activity in the applied tests. They were identified based on 16S rRNA and rpoB, rpoD, or recA gene sequences as Pseudomonas chlororaphis, Citrobacter murliniae, Acinetobacter calcoaceticus, Chryseobacterium lathyri, Chryseobacterium sp., Serratia liquefaciens, and Serratia sp. The culture filtrate of the isolate P. chlororaphis MMC3 L3 04 exerted the strongest inhibitory effect on the tested bacteria. The results of the preliminary study that are presented here, which focused on interactions between the insect gut microbiota and mutualistic bacteria of entomopathogenic nematodes, show that bacteria inhabiting the gut of insects might play a key role in insect resistance to entomopathogenic nematode pressure.

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“…Ek1 Q16 K35 P. chlororaphis is a very strong antifungal agent. Mezaache-Aichour et al (2016) tested the antifungal effects of P. chlororaphis against F. solani in dual culture, and it showed strong antifungal activity and inhibited fungal growth by approximately 65%. Ghosh et al (2014) examined the antifungal effects of Pseudomonas spp.…”

Section: Reduction Of Seeds Fungal Infection (%)mentioning

confidence: 99%

Daucus carrota L. - seed germination and natural infection by Fusarium spp. affected by Pseudomonas spp.

et al. 2017

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The inhibitory effect of indigenous Pseudomonas strains on Fusarium spp. isolated from seeds of a new variety of carrot -'Vizija' and their effects on seed germination were observed. Pseudomonas spp. strains were applied as bacterial suspensions (culture, 10 6 CFU mL -1 ) and cell-free supernatant (CFSa -10 6 and CFSb -10 8 CFU mL -1 ) fractions by sowing seeds during 7 (I) and 14 (II) days of incubation. The germination of control 'Vizija' seeds was 19% after the first and 40% after the second incubation period. Pseudomonas chlororaphis Q16 strain exhibited statistically significant increases in seed germination with all applied fractions and incubation periods, showing values of 43-62% and 55.5-91%, i.e. increments of 24-43% and 15.5-51% compared to the control, after 7 and 14 days of incubation, respectively. P. chlororaphis K35 showed lower but significantly different seed germination values (38-67%) for all variants, except for 7 days old culture. Pseudomonas sp. Ek1 had weaker seed germination potential, showing statistically significant increment only for CFSa,b (I) and CFSb (II) fractions. All tested Pseudomonas strains inhibited the growth of three Fusarium species isolated from 'Vizija' seeds: F. solani, F. oxysporum and F. subglutinans. Natural infection was observed in 20% (I) and 54% (II) of 'Vizija' seeds. P. chlororaphis K35 was the most efficient antifungal strain, reducing seed infection 97.5-100%, followed by Q16 with 95-100%, showing no statistically significant mutual difference. Pseudomonas sp. Ek1 showed a weaker antifungal activity and reduced seed infection by 85-96.75%. The application of P. chlororaphis Q16 and K35 as strains effective in improvement of carrot seed germination and growth inhibition of the seed pathogens F. solani, F. oxysporum and F. subglutinans, can be further tested in carrot production for more beneficial effects.

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Antimicrobial Activity of Potato Rhizospheric Pseudomonas chlororaphis subsp. aureofaciens from Sétif Algeria

Cited by 4 publications

References 16 publications

Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blight

Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blight

Bacteria from the Midgut of Common Cockchafer (Melolontha melolontha L.) Larvae Exhibiting Antagonistic Activity Against Bacterial Symbionts of Entomopathogenic Nematodes: Isolation and Molecular Identification

Daucus carrota L. - seed germination and natural infection by Fusarium spp. affected by Pseudomonas spp.

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