Antimicrobial activity of protein-containing fractions isolated from Lactobacillus plantarum NRRL B-4496 culture

Arrioja-Bretón, D.; Mani‐López, Emma; Bach, Horacio; López‐Malo, Aurelio

doi:10.1007/s42770-020-00266-5

Cited by 16 publications

(15 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CFS did not exhibit cytotoxic activity on human foreskin fibroblast cells at 0.1 to 1000 µg/mL (Moradi, Mardani, & Tajik, 2019). Arrioja‐Bretón, Mani‐López, Bach, and López‐Malo (2020) assessed the cytotoxic and inflammatory activities of CFS fractions from L. plantarum NRRL B‐4496 after identifying its antimicrobial activity and separating the protein fractions exhibiting anti‐ Listeria activity. The antimicrobial fractions contained seven compounds, all of which were nontoxic to THP‐1 cells.…”

Section: Cytotoxicity Of Cfsmentioning

confidence: 99%

The impacts of antimicrobial and antifungal activity of cell‐free supernatants from lactic acid bacteria in vitro and foods

Mani‐López

Arrioja-Bretón

López‐Malo

2021

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Lactic acid bacteria (LAB) are distinguished by their ability to produce lactic acid, among other interesting metabolites with antimicrobial activity. A cell‐free supernatant (CFS) is a liquid containing the metabolites resulting from microbial growth and the residual nutrients of the medium used. CFS from LAB can have antimicrobial activity due to organic acids, fatty acids, and proteinaceous compounds, among other compounds. This review aims to summarize the information about CFS production, CFS composition, and the antimicrobial (antibacterial and antifungal) activity of CFS from LAB in vitro, on foods, and in active packaging. In addition, the mechanisms of action of CFS on cells, the stability of CFS during storage, CFS cytotoxicity, and the safety of CFS are reviewed. The main findings are that CFS's antibacterial and antifungal activity in vitro has been widely studied, particularly in members of the genus Lactobacillus. CFS has produced strong inhibition of bacteria and molds on foods when applied directly or in active packaging. In most studies, the compounds responsible for antimicrobial activity are identified. A few studies indicate that CFSs are stable for 1 to 5 months at temperatures ranging from 4 to 35°C. The cytotoxicity of CFS on human cells has not been well studied. However, the studies that have been performed reported no toxicity of CFS. Therefore, it is necessary to investigate novel growth mediums for CFS preparation that are compatible with food sensory properties. More studies into CFS stability and cytotoxic effects are also needed.

show abstract

Section: Cytotoxicity Of Cfsmentioning

confidence: 99%

The impacts of antimicrobial and antifungal activity of cell‐free supernatants from lactic acid bacteria in vitro and foods

Mani‐López

Arrioja-Bretón

López‐Malo

2021

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

show abstract

“…In the case of Lactobacillus plantarum , some strains (such as KFCC11389P, HY7712, and CAU1055) are considered anti-inflammatory mediators (Chon et al, 2009 ; Jang et al, 2013 ; Choi et al, 2019 ). However, some others (such as NRRL B-4496, RS20D, and Ln1) exhibit antimicrobial activity by promoting pro-inflammatory cytokines or signaling pathways (NF-κB or MAPK), but are non-toxic (Zhu et al, 2019 ; Arrioja-Bretón et al, 2020 ; Jang et al, 2020 ). Even when macrophages are exposed to the same probiotic strain, different results may be obtained, depending on the macrophage status (Habil et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus plantarum synergistically regulates M1 macrophage polarization in resistance against Salmonella enterica serovar Typhimurium infection

et al. 2022

View full text Add to dashboard Cite

Macrophage polarization affects the progression of pathogenic bacterial infections. Lactobacillus is widely used to interact with macrophages and to exert specific immunomodulatory activities. In this study, we investigated the regulation of macrophage polarization against Salmonella enterica serotype Typhimurium (STM) by Lactobacillus plantarum JL01 (LP), to explore prevention and treatment strategies for salmonellosis. We assessed the in vitro differential polarization of RAW 264.7 macrophages and mouse bone marrow macrophages (BMMs) by LP against STM, by measuring protein and cytokine levels, and bactericidal activity. In addition, we assessed the protective effects of LP against STM by evaluating weight loss, survival, the burden of STM in tissues, the polarization of macrophages in the spleen and mesenteric lymph nodes (MLNs), intestinal histopathology, and cytokine production. LP slightly affected the polarization of RAW 264.7, a slight M1-skewing. LP promoted the RAW 264.7 bactericidal activity against STM. In BMMs, M1 polarization induced by LP was significantly lower than the M1-positive phenotype. The combination of LP with M1 synergistically improved M1 polarization and bactericidal activity against STM compared to the individual effects. LP promoted the activation of the NF-κB signaling pathway. Supplementation with the NF-κB inhibitor decreased M1 polarization induced by LP. We observed the protective effect of LP against STM in C57BL/6 mice, through a decrease in weight loss, mortality, STM burden in the liver, and promotion of macrophage M1 and M2 polarization in the spleen and MLNs; though M1 was higher, it did not cause inflammatory damage. In summary, LP can synergistically promote M1 polarization in combination with the M1 phenotype through the NF-κB signaling pathway and increases resistance against S. Typhimurium infection. These findings will lay the foundation for the prevention and treatment of S. Typhimurium infections in the future.

show abstract

“…Indeed, listeriosis can determine sepsis in immunocompromised patients, meningoencephalitis and febrile gastroenteritis [ 112 ]. The activity of these proteins against L. monocytogenes, sometimes regardless of pH [ 113 ], besides probiotic properties and safety of the producing strain, allow the development of novel bio-preservatives, with potential use in the food industry. At present, only two bacteriocins have been given the GRAS status, being approved for use as natural food preservatives, both produced by LAB (i.e., nisin, from Lactococcus , and pediocin PA-1D, from Pediococcus genus) [ 114 ].…”

Section: Antibacterial and Antiviral Spectrum Of L Plantarum Extracellular Compoundsmentioning

confidence: 99%

“…For instance, MRSA accounted for 16% of necrotising soft tissue infections worldwide, although overall mortality is declining over the last ten years [ 117 , 118 ]. Examples of antimicrobials against these resistant forms comprise the CFS of L. plantarum strains extracted from sauerkraut [ 113 ] and from faecal microbiota [ 102 ], plantaricins LpU4 and ZJ008 from L. plantarum strains isolated from milk [ 54 , 59 ]. These extracellular compounds and their strains may represent an alternative bio-control strategy against skin infections.…”

Section: Antibacterial and Antiviral Spectrum Of L Plantarum Extracellular Compoundsmentioning

confidence: 99%

Bioprospecting Antimicrobials from Lactiplantibacillus plantarum: Key Factors Underlying Its Probiotic Action

Rocchetti

Russo

Capozzi

et al. 2021

IJMS

View full text Add to dashboard Cite

Lactiplantibacillus plantarum (L. plantarum) is a well-studied and versatile species of lactobacilli. It is found in several niches, including human mucosal surfaces, and it is largely employed in the food industry and boasts a millenary tradition of safe use, sharing a long-lasting relationship with humans. L. plantarum is generally recognised as safe and exhibits a strong probiotic character, so that several strains are commercialised as health-promoting supplements and functional food products. For these reasons, L. plantarum represents a valuable model to gain insight into the nature and mechanisms of antimicrobials as key factors underlying the probiotic action of health-promoting microbes. Probiotic antimicrobials can inhibit the growth of pathogens in the gut ensuring the intestinal homeostasis and contributing to the host health. Furthermore, they may be attractive alternatives to conventional antibiotics, holding potential in several biomedical applications. The aim of this review is to investigate the most relevant papers published in the last ten years, bioprospecting the antimicrobial activity of characterised probiotic L. plantarum strains. Specifically, it focuses on the different chemical nature, the action spectra and the mechanisms underlying the bioactivity of their antibacterial and antiviral agents. Emerging trends in postbiotics, some in vivo applications of L. plantarum antimicrobials, including strengths and limitations of their therapeutic potential, are addressed and discussed.

show abstract

Antimicrobial activity of protein-containing fractions isolated from Lactobacillus plantarum NRRL B-4496 culture

Cited by 16 publications

References 34 publications

The impacts of antimicrobial and antifungal activity of cell‐free supernatants from lactic acid bacteria in vitro and foods

The impacts of antimicrobial and antifungal activity of cell‐free supernatants from lactic acid bacteria in vitro and foods

Lactobacillus plantarum synergistically regulates M1 macrophage polarization in resistance against Salmonella enterica serovar Typhimurium infection

Bioprospecting Antimicrobials from Lactiplantibacillus plantarum: Key Factors Underlying Its Probiotic Action

Contact Info

Product

Resources

About