Antimicrobial and anticoagulation activity of silver nanoparticles synthesized from the culture supernatant of Pseudomonas aeruginosa

Jeyaraj, M.; Varadan, Senthilkumar; Anthony, Kevin John Pulikotil; Muralidharan, M.; A, Raja; Gurunathan, Sangiliyandi

doi:10.1016/j.jiec.2012.12.031

Cited by 41 publications

(16 citation statements)

References 39 publications

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“…Several researchers studied the average size of AgNPs synthesized using culture supernatant of P. aeruginosa were 50-80 nm. An average size of AgNPs prepared in the present study is 40 nm, which is lower than the previous studies (Jeevan et al 2012;Jeyaraj et al 2013).…”

Section: Characterization Of Agnpscontrasting

confidence: 73%

Bactericidal potential of silver nanoparticles synthesized using cell-free extract of Comamonas acidovorans: in vitro and in silico approaches

Rudakiya

Pawar²

2017

3 Biotech

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The need to overcome human threats from pathogenic microbes, development of nanomaterials have been provoked for a new generation of antimicrobials. In the present study, biosynthesis of silver nanoparticles (AgNPs) was acquired using Comamonas acidovorans extract within 72 h under static condition. Electron microscopy studies revealed that the size of AgNPs was ranging from 6-53 nm and had spherical, oval and irregular shapes with smooth surfaces. Prepared AgNPs interacted with proteins, carbohydrates and other aromatic molecules. Biosynthesized AgNPs were bactericidal, which significantly inhibited pathogenic microbes, i.e., Streptococcus pyogenes, Staphylococcus aureus and Escherichia coli. Higher concentrations of AgNPs (20 lg ml -1 ) inhibited 92-98% growth of all tested bacteria within 24 h. AgNPs-protein network studies carried out to recognize the protein interactions with AgNPs and to understand probable bactericidal mechanisms. AgNPs may penetrate into cell through membrane proteins and damage them by modifying amino acids. Due to AgNPs-protein interactions, dysfunctions in enzymes obstruct certain metabolic processes, which cause the bacteria to die eventually. In certain pathogenic microbes, cue and cus systems detoxify Ag ? ions, transport through transporter proteins and expel them to the extracellular space, which are mainly responsible for Ag resistance.

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Section: Characterization Of Agnpscontrasting

confidence: 73%

Bactericidal potential of silver nanoparticles synthesized using cell-free extract of Comamonas acidovorans: in vitro and in silico approaches

Rudakiya

Pawar²

2017

3 Biotech

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“…Klebsiella pneumonia , responsible for nosocomial pneumonia in “immunoincompetent” people, produces approximately 10 % of all infections that are acquired in hospitals including urinary tract infections, pneumonia, surgical wounds, and biliary tract wounds (Tam 2013). The most important Gram-negative bacterium is P. aeruginosa , which has become a significant cause of infection, particularly in patients with compromised host defense mechanisms (Tepe et al 2004; Jeyaraj et al 2013). Pseudomonas aeruginosa is the main and usual pathogen isolated from patients who have been hospitalized for longer than 1 week (Ahmed et al 2013).…”

Section: Resultsmentioning

confidence: 99%

Antibacterial, antioxidant, antifungal and anti-inflammatory activities of crude extract from Nitraria schoberi fruits

et al. 2014

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This study is the first comprehensive investigation of the antibacterial, antioxidant, antifungal and anti-inflammatory activities of a crude extract from Nitraria schoberi L. (Zygophyllaceae) fruits. The extract was tested against four Gram-negative (Pseudomonas aeruginosa, Enterobacter aerogenes, Klebsiella pneumoniae and Acinetobacter lwoffii) and one Gram-positive (Staphylococcus aureus) bacteria using the agar disc diffusion and microdilution methods. P. aeruginosa was inhibited the most (widest inhibition zone) while K. pneumonia showed the largest MIC value. The antioxidant activity of fruits (0.02 mg/mL vs. 0.04, 0.06 and 1.00 mg/mL for α-tocopherol, butylated hydroxyanisole and ascorbic acid, respectively) was determined by the paired diene method. The antifungal activity of N. schoberi fruits was tested against different fungi, including Aspergillus niger and Candida albicans, with 300 µg/mL of fruit extract being the most effective concentration. The percentage of anti-inflammatory activity assayed for N. schoberi fruit extract at 100, 200 and 500 µg/mL was 36.12, 59.89 and 88.33 %, respectively. N. schoberi fruits possess potent antibacterial, antioxidant, antifungal and anti-inflammatory properties, and may be used as an antibacterial and antifungal to treat diseases and/or as a protective agent against disorders associated with oxidative stress and inflammation.

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“…Therefore, Pseudomonas and Staphylococcus were selected for further analysis. To identify dominant bacterial species like Pseudomonas species and Staphylococcus species, 16s rRNA gene sequence analysis was performed according to a previously described method [94]. …”

Section: Methodsmentioning

confidence: 99%

Effects of Silver Nanoparticles on Multiple Drug-Resistant Strains of Staphylococcus aureus and Pseudomonas aeruginosa from Mastitis-Infected Goats: An Alternative Approach for Antimicrobial Therapy

Yuan

Peng

Gurunathan

2017

IJMS

Self Cite

270

177

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Recently, silver nanoparticles (AgNPs) have been widely used in various applications as antimicrobial agents, anticancer, diagnostics, biomarkers, cell labels, and drug delivery systems for the treatment of various diseases. Microorganisms generally acquire resistance to antibiotics through the course of antibacterial therapy. Multi-drug resistance (MDR) has become a growing problem in the treatment of infectious diseases, and the widespread use of broad-spectrum antibiotics has resulted in the development of antibiotic resistance by numerous human and animal bacterial pathogens. As a result, an increasing number of microorganisms are resistant to multiple antibiotics causing continuing economic losses in dairy farming. Therefore, there is an urgent need for the development of alternative, cost-effective, and efficient antimicrobial agents that overcome antimicrobial resistance. Here, AgNPs synthesized using the bio-molecule quercetin were characterized using various analytical techniques. The synthesized AgNPs were highly spherical in shape and had an average size of 11 nm. We evaluated the efficacy of synthesized AgNPs against two MDR pathogenic bacteria, namely, Pseudomonas aeruginosa and Staphylococcus aureus, which were isolated from milk samples produced by mastitis-infected goats. The minimum inhibitory concentrations (MICs) of AgNPs against P. aeruginosa and S. aureus were found to be 1 and 2 μg/mL, respectively. Our findings suggest that AgNPs exert antibacterial effects in a dose- and time-dependent manner. Results from the present study demonstrate that the antibacterial activity of AgNPs is due to the generation of reactive oxygen species (ROS), malondialdehyde (MDA), and leakage of proteins and sugars in bacterial cells. Results of the present study showed that AgNP-treated bacteria had significantly lower lactate dehydrogenase activity (LDH) and lower adenosine triphosphate (ATP) levels compared to the control. Furthermore, AgNP-treated bacteria showed downregulated expression of glutathione (GSH), upregulation of glutathione S-transferase (GST), and downregulation of both superoxide dismutase (SOD) and catalase (CAT). These physiological and biochemical measurements were consistently observed in AgNP-treated bacteria, thereby suggesting that AgNPs can induce bacterial cell death. Thus, the above results represent conclusive findings on the mechanism of action of AgNPs against different types of bacteria. This study also demonstrates the promising use of nanoparticles as antibacterial agents for use in the biotechnology and biomedical industry. Furthermore, this study is the first to propose the mode of action of AgNPs against MDR pathogens isolated from goats infected with subclinical mastitis.

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Antimicrobial and anticoagulation activity of silver nanoparticles synthesized from the culture supernatant of Pseudomonas aeruginosa

Cited by 41 publications

References 39 publications

Bactericidal potential of silver nanoparticles synthesized using cell-free extract of Comamonas acidovorans: in vitro and in silico approaches

Bactericidal potential of silver nanoparticles synthesized using cell-free extract of Comamonas acidovorans: in vitro and in silico approaches

Antibacterial, antioxidant, antifungal and anti-inflammatory activities of crude extract from Nitraria schoberi fruits

Effects of Silver Nanoparticles on Multiple Drug-Resistant Strains of Staphylococcus aureus and Pseudomonas aeruginosa from Mastitis-Infected Goats: An Alternative Approach for Antimicrobial Therapy

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