Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens

Ahmad, Iqbal; Beg, Arina Z.

doi:10.1016/s0378-8741(00)00335-4

Cited by 907 publications

(552 citation statements)

References 22 publications

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confidence: 99%

“…In vitro studies on plants used in traditional medicine have been carried out in the field of microbiology, especially on pathogenic bacterial growth; and some of these studies were about the antimicrobial activity of Mikania glomerata Spreng ("guaco") (Boyayan 2002, Holetz et al 2002, Psidium guajava L (guava) (Gnan & Demello 1999, Jaiarj et al 1999, Nascimento et al 2000, Ahmad & Beg 2001, Abdelrahim et al 2002, Holetz et al 2002, Voravuthikunchai et al 2004, Qadan et al 2005 (Ankri & Mirelman 1999, Ahmad & Beg 2000, Srinivasan et al 2001, Benkeblia 2004, Zingiber officinale Roscoe (ginger) (Silva Junior et al 1994, Konning et al 2004, Cymbopogon citratus (DC) Stapf (lemongrass) (Cimanga et al 2002, Di Stasi & HirumaLima 2002, Mentha piperita L (mint) (Silva Junior et al 1994, Tassou et al 2000, and Baccharis trimera (Less.) DC ("carqueja") (Avancini et al 2000).…”

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Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases

Betoni¹,

Mantovani²,

Barbosa³

et al. 2006

Mem. Inst. Oswaldo Cruz

282

160

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Searches for substances with antimicrobial activity are frequent, and medicinal plants have been considered interesting by some researchers since they are frequently used in popular medicine as remedies for many infectious diseases. The aim of this study was to verify the synergism between 13 antimicrobial drugs and 8 plant extracts -"guaco" (Mikania glomerata), guava (Psidium guajava), clove (Syzygium aromaticum), garlic (Allium sativum), lemongrass (Cymbopogon citratus), ginger (Zingiber officinale), "carqueja" (Baccharis trimera), and mint (Mentha piperita) -against Staphylococcus aureus strains, and for this purpose, the disk method was the antimicrobial susceptibility test performed. Petri dishes were prepared with or without dilution of plant extracts at sub-inhibitory concentrations in Mueller-Hinton Agar (MHA) In a constant attempt to improve their quality of life, men have used plants as source of food, shelter, clothing, medicine, cosmetics, and for seeking relief from hardship of life. Some plants are known as medicinal because they contain active substances that cause certain reactions, from relenting to the cure of diseases, on the human organism (Silva Junior et al. 1994). Knowledge on medicinal plants sometimes means the only therapeutic resource of some communities and ethnic groups (Di Stasi 1996); and their use, especially in South America, contributes significantly to primary health care (Holetz et al. 2002). Infectious diseases still represent an important cause of morbidity and mortality among humans, especially in developing countries. Even though pharmaceutical industries have produced a number of new antimicrobial drugs in the last years, resistance to these drugs by microorganisms has increased. In general, bacteria have the genetic ability to transmit and acquire resistance to drugs used as therapeutic agents (Nascimento et al. 2000).In vitro studies on plants used in traditional medicine have been carried out in the field of microbiology, especially on pathogenic bacterial growth; and some of these studies were about the antimicrobial activity of Mikania glomerata Spreng ("guaco") (Boyayan 2002, Holetz et al. 2002, Psidium guajava L (guava) (Gnan & Demello 1999, Jaiarj et al. 1999, Nascimento et al. 2000, Ahmad & Beg 2001, Abdelrahim et al. 2002, Holetz et al. 2002, Voravuthikunchai et al. 2004, Qadan et al. 2005, Syzygium MATERIALS AND METHODSPlant samples -M. glomerata, P. guajava, B. trimera, M. piperita, and C. citratus samples were collected in 2004 from an experimental field of the School of Agronomical Sciences, Unesp, Botucatu, São Paulo, Brazil, and the voucher specimens were deposited at the Herbarium of the Department of Botany, Institute of Biosciences, Unesp. Their leaves were dried at 40 o C and triturated in a mechanical mill. A. sativum, S. aromaticum, and Z. officinale samples were obtained from the local commerce in the same year and were used in natura for the extracts preparation. Preparation of plant extracts -Plant material, dried (M. glomerata, P. guajava, B. ...

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confidence: 99%

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confidence: 99%

Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases

Betoni¹,

Mantovani²,

Barbosa³

et al. 2006

Mem. Inst. Oswaldo Cruz

282

160

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“…The antibacterial activity of cAgNPs was assayed against Vibrio harveyi by using agar well diffusion method (Ahmad and Beg 2001). In brief, Mueller-Hinton Agar (MHA) plates were inoculated uniformly with 1.5 9 10 8 CFU/mL (0.5 McFarland Standard) of bacterial suspension, using sterile swabs in triplicates.…”

Section: Zone Of Inhibition (Zoi) Assaymentioning

confidence: 99%

Assessment of antibacterial activity of two different sizes of colloidal silver nanoparticle (cAgNPs) against Vibrio harveyi isolated from shrimp Litopenaeus vannamei

et al. 2016

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Silver nanoparticles are the most important nanomaterials for antibacterial uses and are famous for their strong inhibitory and antibacterial effects. In recent years, extensive studies have been undertaken on the use of antimicrobial properties of silver, incorporated within aquaculture industry. To evaluate the scientific basis for the use of the nano silver in shrimp aquaculture, in this study the antimicrobial activities of colloidal nano silver with two different sizes (16.62 and 22.22 nm) was evaluated against gram negative bacteria, V. harveyi. Before the experiments, cAgNPs were characterized using several analytical techniques. Well diffusion method, micro-dilution tests (MIC and MBC) and kinetic of death were used to evaluate the bactericidal activity of the nanoparticles. Results showed that MIC and MBC values of cAgNPs in both studied sizes are equal (MIC = MBC). Best bactericidal kinetics in the presence of 16.62 and 22.22 nm nanoparticles obtained at 4 and 6 h, respectively. The obtained results suggested that smaller silver nanoparticles had a faster antibacterial activity than the larger particles. According to the obtained results, the activity of cAgNPs against V. harveyi is fast and has potential for the treatment of bacterial infection in aquaculture.

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“…It has been demonstrated that the stem and leaves of O. sanctum possess several bioactive compounds such as saponins, flavonoids, triterpenoids, tannins 9 as well as phenolic compounds 10 , which have great therapeutic importance in curing many diseases, and are also responsible for antimicrobial activity 11,12 . Possessing enormous number of bioactive components 6,13 , O. sanctum potentiality inhibit the growth of several bacterial pathogens including Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Salmonella enterica serovar Typhi (S. typhi), Staphylococcus aureus (Staph.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Bacterial Growth Inhibition Property and Phytochemical Analysis of Ocimum Sanctum L. Leaf Extract

Das¹,

Mandal²,

Mandal³

2017

Int. Res. J. Pharm.

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Tulsi plant, Ocimum sanctum L., is famous for its therapeutic potentiality. The present study investigates the antibacterial activity of O. sanctum leaf extracts, and the bioactive components present in the extracts. The antibacterial activity of ethanolic leaf extract of O. sanctum: dark variety; Krishna tulsi (DOSE) and bright variety; Rama tulsi (BOSE), and aqueous leaf extract of dark variety (AqDOS) and bright variety (AqBOS) of O. sanctum was determined by in vitro methods against gram-negative and gram-positive clinical bacterial isolates. Phytochemical screening of the extracts was done qualitatively, and thin layer chromatography (TLC) was performed using n-hexane-ethyl acetate mobile phase. The ethanolic extracts showed more antibacterial activity as compared to aqueous extracts in terms of ZDI (zone diameter of inhibition); the ZDI of DOSE ranged 20 -28 mm for gram-positive, and 14 -23 mm for gram-negative bacteria, whereas in case of BOSE the ZDIs ranged 11 -22 mm and 12 -18 mm, respectively, for gram-positive and gram-negative bacteria. The Rf values of phytocomponents detected on the TLC plate, ranged 0.06 -0.94. The O. sanctum leaf extracts had broad spectrum antibacterial activity and found rich source of phytochemicals, which thus, be utilized against a broad range of bacterial infection.

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Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens

Cited by 907 publications

References 22 publications

Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases

Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases

Assessment of antibacterial activity of two different sizes of colloidal silver nanoparticle (cAgNPs) against Vibrio harveyi isolated from shrimp Litopenaeus vannamei

Assessment of Bacterial Growth Inhibition Property and Phytochemical Analysis of Ocimum Sanctum L. Leaf Extract

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