Antimicrobial, Antioxidant and Cytotoxic Activity of MarineStreptomyces parvulus VITJS11 Crude Extract

Naine, S. Jemimah; Devi, C. Subathra; Mohanasrinivasan, V.; Vaishnavi, B.

doi:10.1590/s1516-8913201400173

Cited by 22 publications

(11 citation statements)

References 22 publications

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“…The presence of antifungal activities is also an indication of probable biocontrol mechanisms against mycotoxin producing fungal strains. Similar findings have been reported in similar studies of Streptomyces strains (Rahman et al, 2010 ; Usha et al, 2010 ; Naine et al, 2015 ).…”

Section: Discussionsupporting

confidence: 90%

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Characterization and evaluation of antimicrobial and cytotoxic effects of Streptomyces sp. HUST012 isolated from medicinal plant Dracaena cochinchinensis Lour.

et al. 2015

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A highly potent secondary metabolite producing endophytic strain, Streptomyces sp. HUST012 was isolated from the stems of the medicinal plant Dracaena cochinchinensis Lour. Strain HUST012 showed antimicrobial and antitumor activities which were significantly much higher than those of dragon's blood extracted from D. cochinchinensis Lour. On further analysis, the strain was found to produce two metabolites, SPE-B11.8 (elucidated to be a novel metabolite (Z)-tridec-7-ene-1,2,13-tricarboxylic acid) and SPE-B5.4 (elucidated as Actinomycin-D). The Minimum Inhibitory Concentration values of SPE-B11.8 against a set of test bacterial organisms (Methicillin-resistant Staphylococcus epidermis ATCC 35984, Methicillin-resistant Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 13883) ranged between 15.63 and 62.5 μg/ml while that for SPE-B5.4 ranged between 0.04 and 2.24 μg/ml. The compound SPE-B11.8 showed cytotoxic effect at 41.63 and 29.54 μg/ml IC50-values against Hep G2 and MCF-7, respectively, while the compound SPE-B5.4 exhibited stronger activities against them at 0.23 and 0.18 μg/ml IC50-values.

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Section: Discussionsupporting

confidence: 90%

“…The cytotoxic ability of this strain was significant as compared to that reported in previous studies on S. parvulus strain VITJS11 (Naine et al, 2015 ). The compounds SPE-B11.8 and SPE-B5.4 had I C 50 -values of 41.63 and 0.23 μg/ml on Hep G2 cells as compared to 500 μg/ml by S. parvulus strain VITJS11.…”

Section: Discussionmentioning

confidence: 38%

Characterization and evaluation of antimicrobial and cytotoxic effects of Streptomyces sp. HUST012 isolated from medicinal plant Dracaena cochinchinensis Lour.

et al. 2015

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“…Of the 17 strains selected, 10 strains were significant against F. graminearum , seven against A. carbonarius , and four against A. westerdijkiae . Similar findings have been reported in related studies of Streptomyces strains [42–44]. Four strains (HUST003, HUST004, HUST005, and HUST026) showed remarkable antifungal activity against all test fungi (Table 5).…”

Section: Discussionsupporting

confidence: 89%

“…This finding is significant with reference to related studies [44–47]. Lu and Shen [45] isolated naphthomycin K from endophytic Streptomyces strain CS which exhibit cytotoxic activity against P388 and A-549 cell lines with IC 50 -values of 0.07 and 3.17 μ mol·L −1 .…”

Section: Discussionsupporting

confidence: 59%

Endophytic Actinobacteria Associated with Dracaena cochinchinensis Lour.: Isolation, Diversity, and Their Cytotoxic Activities

Salam

Khieu

Liu

et al. 2017

BioMed Research International

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Dracaena cochinchinensis Lour. is an ethnomedicinally important plant used in traditional Chinese medicine known as dragon's blood. Excessive utilization of the plant for extraction of dragon's blood had resulted in the destruction of the important niche. During a study to provide a sustainable way of utilizing the resources, the endophytic Actinobacteria associated with the plant were explored for potential utilization of their medicinal properties. Three hundred and four endophytic Actinobacteria belonging to the genera Streptomyces, Nocardiopsis, Brevibacterium, Microbacterium, Tsukamurella, Arthrobacter, Brachybacterium, Nocardia, Rhodococcus, Kocuria, Nocardioides, and Pseudonocardia were isolated from different tissues of D. cochinchinensis Lour. Of these, 17 strains having antimicrobial and anthracyclines-producing activities were further selected for screening of antifungal and cytotoxic activities against two human cancer cell lines, MCF-7 and Hep G2. Ten of these selected endophytic Actinobacteria showed antifungal activities against at least one of the fungal pathogens, of which three strains exhibited cytotoxic activities with IC50-values ranging between 3 and 33 μg·mL−1. Frequencies for the presence of biosynthetic genes, polyketide synthase- (PKS-) I, PKS-II, and nonribosomal peptide synthetase (NRPS) among these 17 selected bioactive Actinobacteria were 29.4%, 70.6%, and 23.5%, respectively. The results indicated that the medicinal plant D. cochinchinensis Lour. is a good niche of biologically important metabolites-producing Actinobacteria.

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“…Actinobacteria are the most prominent bioactive compound producers; they produce approximately two-thirds of the all-natural antibiotics used in medicine and agriculture. Among them, the genus Streptomyces has produced the majority of these compounds with wide range of pharmaceutical activities, such as antimicrobial, anticancer and antiviral activities 5 .…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Marine Actinomycetes and the Optimization of Culture Conditions for the Production of Antimicrobial Agent(s)

Hamed¹,

Abdelfattah²,

Fahmy³

2019

J Pure Appl Microbiol

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Citation: Moaz M. Hamed, Lamis Sh. Abdelfattah and Nayer M. Fahmy, Antimicrobial Activity of Marine Actinomycetes and the Optimization of Culture Conditions for the Production of Antimicrobial Agent(s), J Pure Appl Microbiol., 2019; 13(4):2177-2188. Abstract The aim of the present study was to isolate antagonistic actinomycetes from marine sediment collected from the Red Sea coast at Hurghada city and the Suez Gulf. A total of 16 actinomycete isolates were obtained in October 2018 and their antagonistic activities were evaluated against Aeromonas hydrophila, Vibrio damsela, Staphylococcus aureus ATCC6538, Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa ATCC 9027, Salmonella typhimurium ATCC 14028, Escherichia coli ATCC 19404 and Candida albicans ATCC 10231 by using the agar well diffusion method. Among the 16 isolates, 14 (87.5%) isolates exhibited antimicrobial activity against most of the tested pathogens. The most potent isolate was identified by 16S rRNA sequence analysis as Streptomyces sp. and designated Streptomyces sp. MK388207. The experimental design of Plackett-Burman was implemented to optimize the culture conditions for antimicrobial agent(s) production by the most powerful isolate against C. albicans ATCC 10231 when grown in starch nitrate broth and the data revealed that negative (-) levels of KNO 3 , K 2 HPO 4 and pH and positive (+) levels of starch, MgSO 4 .7H 2 O, FeSO 4 and incubation period supported the production of the antimicrobial agent(s). Growth under the optimized culture conditions led to a 1.4-fold rise in antimicrobial activity. The ethyl acetate extract of Streptomyces sp.MK388207 was subjected to gasliquid chromatography mass spectrometry (GC MS) and revealed the presence of six fractions with the major component being phenol, 2,4-bis (1,1 dimethylethyl). The findings of this study suggested that the antagonistic marine Streptomyces, in particular Streptomyces sp MK388207, the antibacterial compounds produced by this isolate, could be used as antibiotics that could have future applications in the pharmaceutical industry.

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Antimicrobial, Antioxidant and Cytotoxic Activity of MarineStreptomyces parvulus VITJS11 Crude Extract

Abstract: ABSTRACT

Cited by 22 publications

References 22 publications

Characterization and evaluation of antimicrobial and cytotoxic effects of Streptomyces sp. HUST012 isolated from medicinal plant Dracaena cochinchinensis Lour.

Characterization and evaluation of antimicrobial and cytotoxic effects of Streptomyces sp. HUST012 isolated from medicinal plant Dracaena cochinchinensis Lour.

Endophytic Actinobacteria Associated with Dracaena cochinchinensis Lour.: Isolation, Diversity, and Their Cytotoxic Activities

Antimicrobial Activity of Marine Actinomycetes and the Optimization of Culture Conditions for the Production of Antimicrobial Agent(s)

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