Antimicrobial Effect of Eco- Friendly Silver Nanoparticles Synthesis by Iraqi Date Palm (Phoenix dactylifera) on Gram-Negative Biofilm-Forming Bacteria

Abstract

Date palm silver nanoparticles are a green synthesis method used as antibacterial agents. Today, there is a considerable interest in it because it is safe, nontoxic, low costly and ecofriendly. Biofilm bacteria existing in marketed local milk is at highly risk on population health and may be life-threatening as most biofilm-forming bacteria are multidrug resistance. The goal of current study is to eradicate biofilm-forming bacteria by alternative treatment green synthesis silver nanoparticles. The biofilm formation by bacterial isolates was detected by Congo red method. The silver nanoparticles were prepared from date palm(khestawy) fruit extract. The formed nanoparticles were characterized with UV-Vis and AFM. The antibacterial activity of synthetic silver nanoparticles was evaluated by agar well diffusion method. Gram-negative bacteria isolates were E. coli in 3 isolates and Klebsiella pneumoniae in 5 isolates and all are biofilm producer. The size of synthetic green silver nanoparticles is 18 nm and the generation of silver nanoparticles was confirmed by change of date extract color from yellow to brown with an absorption maximum at 410 nm. Highly antibacterial activity of silver nanoparticles was recorded in comparison to plant extract and silver nitrate against gram-negative biofilm-forming bacteria. From this study, the antibacterial activity of date palm silver nanoparticles was more efficient to eradicate gram negative biofilm[1]forming bacteria isolated from marketed local milk

Silver Nanoparticles Synthesized by Cold Plasma as an Antibiofilm Agent against Staphylococcus epidermidis Isolated from Acne

“…The characteristic absorption band of AgNPs typically falls within the range of 350-450 nm. The asymmetrical and narrow SPR band suggests that the AgNPs possess a uniform shape and a narrow size distribution [21][22][23]. Upon these facts and regarding the results illustrated in Fig.…”

النشاط المضاد للميكروبات لجسيمات الفضة النانوية على البكتيريا المسببة للأمراض المعزولة من الحالات السريرية لعدوى المستشفيات

“…The mixture was heated and the colourless solution changed to yellowish brown. The solution was the removed from microwave oven and still at room temperature with stirring to cool at 28ᴼC 17 .…”

Preparing a New Type of Concrete Based on Sulfur-melamine Modifier

“…The chemical and corrosion stability of this concrete was investigated in the various aggressive chemical and corrosion solutions. Before doing experiment, the prepared concrete was submerged in the selected aggressive chemical and corrosion solutions for 60 days [44][45][46] . The values of stability coefficient were measured in this experiment.…”