Antimicrobial effect of ozonated water, sodium hypochlorite and chlorhexidine gluconate in primary molar root canals

Abstract

Objective:The aim was to determine the antimicrobial effect of ozonated water, ozonated water with ultrasonication, sodium hypochloride and chlorhexidine (CHX) in human primary root canals contaminated by Enterococcus faecalis (E. faecalis).Materials and Methods:Fifty-eight extracted human primary molar teeth were used. Crowns were cut off using a diamond saw under water-cooling. One hundred roots were obtained and mechanically prepared. The roots were then sterilized by autoclaving in water for 15 min at 121°C. All samples were contaminated with E. faecalis for 24 h and the root canals were randomly divided into five groups (n = 20). Group I: 25 mg/L of Ozonated water (O3aq), Group II: 25 mg/L of O3aq with ultrasonication, Group III: 2.5% Sodium hypochloride (NaOCl), Group IV: 2% CHX and Group V: Positive control. The canal of each specimen was irrigated for 4 min and positive control was untreated. All root canals were agitated with sterile saline solution. The saline solution was collected from canals with sterile paper points. For each specimen, the paper points were transposed to eppendorf vials containing 2 ml of brain heart infusion. According to bacterial proliferation, the mean values of optical density were achieved by ELİSA (Biotek EL ×800, Absorbance Microplate Reader, ABD) and the data were analyzed.Results:NaOCI, CHX and two types of O3aq were found statistically different than positive control group. NaOCI irrigation was found significantly most effective.Conclusions:NaOCl, CHX and O3aq applications provide antibacterial effect in vitro conditions in primary root canals.

Comparison of the effect of MTAD and conventional irrigation agents on apical leakage and push‐out bond strength of root canal filling

“…Different solutions are used for root canal irrigation. NaOCl is the most preferred irrigation solution because it is an organic solvent, antiseptic, has low surface tension, can easily diffuse to dentin walls, shows good residual effect, and has a long shelf life (Frais et al ., ; Goztas et al ., ; Tulsani et al ., ). The cleaning and antimicrobial effect of EDTA and NaOCl combined is superior to using a single solution (Niu et al ., ; Kalyoncuoglu and Demiryurek, ).…”

Antimicrobial efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine, and ozonated water as irrigants in mesiobuccal root canals with severe curvature of mandibular molars

“…Ozone's oxidizing power is exerted specifically on polyunsaturated fatty acids of the bacterial membrane, increasing oxygen delivery to tissues and modulating the immune system, thereby improving and accelerating tissue repair. According to Goztas et al .,[ 33 ] the advantages of ozone in the aqueous phase include lack of mutagenicity, rapid microbicidal effects, and ease of handling. The authors also reported that ozonated water shows no cytotoxicity and is highly biocompatible compared with other antimicrobial agents.…”

Comparación entre clorhexidina e hipoclorito de sodio como soluciones desinfectantes en la práctica endodóntica

“…Evaluando la eficacia antimicrobiana del extracto de hoja de guayaba y aloe vera en comparación con hipoclorito de sodio, clorhexidina y QMix empleados como irrigantes de conductos radiculares, observamos que si bien el extracto de hojas de guayaba y el extracto áloe vera poseen acciones antibacterianas y antioxidantes contra patógenos orales Candida Albicans y Enterococus Faecalis, los resultados muestran al QMix como una nueva propuesta para la irrigación de conductos relacionando su eficacia antibacteriana con su composición (11) .…”