Antimicrobial, modulatory and chemical analysis of the oil of
            <i>Croton limae</i>

Leite, Tiago Rodrigues; Silva, Maria Arlene Pessôa da; Santos, Antonio; Coutinho, Henrique Douglas Melo; Duarte, Antônia Eliene; Costa, José Galberto Martins da

doi:10.1080/13880209.2017.1355926

Cited by 16 publications

(9 citation statements)

References 29 publications

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“…Accordingly, different classes of natural products have been shown to be able to modulate resistance to azoles [ 67 ]. The present study demonstrated the modulating effects of the ethanolic extract of C. heliotropiifolius roots, corroborating previous work that found similar effects for plants of the same genus against Candida strains [ 69 , 70 ].…”

Section: Discussionsupporting

confidence: 92%

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Chemical Constituents and Biological Activities of Croton heliotropiifolius Kunth

et al. 2021

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Croton heliotropiifolius Kunth (Euphorbiaceae), whose occurrence has already been registered in the most varied Brazilian biomes, is commonly found in the Chapada do Araripe, Ceará. The species is traditionally used to treat fungal, parasitic, and degenerative diseases. This study investigated the chemical composition and pharmacological potential (antioxidant, antifungal, antiparasitic, and cytotoxic) of an aqueous extract obtained from the roots of C. heliotropiifolius. Following a qualitative phytochemical screening, the chemical constituents were identified by ultra-efficiency liquid chromatography coupled witha quadrupole/time-of-flight system (UPLC-QTOF). The antioxidant potential was verified by thin-layer chromatography (TLC). The direct and combined antifungal activity of the extract against opportunistic Candida strains was investigated using the microdilution method. The minimal fungicidal concentration (MFC) was determined by subculture, while the modulation of the morphological transition (fungal virulence) was evaluated by light microscopy. The in vitro antiparasitic activity was analyzed using epimastigotes of Trypanosoma cruzi and promastigotes of Leishmania braziliensis and Leishmania infantum, while cytotoxicity was determined in cultures of mouse fibroblasts. The phytochemical analysis identified the presence of acids, terpenes, flavonoids, lignans, and alkaloids. Among these constituents, the presence of polar and non-polar phenolic compounds with known antioxidant action washighlighted. While the extract showed clinically ineffective antifungal effects, it could enhance the effectiveness of fluconazole, in addition to inhibiting the morphological transition associated with increased virulence in Candida strains. Although the extract showed low cytotoxicity against fibroblasts, it also had weak antiparasitic effects. In conclusion, Croton heliotropiifolius is a source of natural products with antifungal and antioxidant potential.

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Section: Discussionsupporting

confidence: 92%

“…Based on previous studies, it is reasonable to assume that this effect may be due to the presence of components such as carboxylic acids, terpenes [ 55 , 58 ], and phenolic compounds [ 69 ]. However, further research is required to investigate the action of isolated compounds.…”

Section: Discussionmentioning

confidence: 99%

Chemical Constituents and Biological Activities of Croton heliotropiifolius Kunth

et al. 2021

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“…Similarly to what observed by Pinheiro et al [2], HP-β-CD showed an endothermic peak ( Figure 6B) with Tonset = 37.25 °C (ΔH = 6.47 J/g and Tpeak = 37.05 °C) related to water release from its β-CD exhibited an endothermic peak ( Figure 6A) involving ∆H = 231.02 J/g with Tonset = 30.94 • C and Tpeak = 73.71 • C, likely due to water release from its cavity, and a second event occurred at Tonset = 223.50 • C (∆H = 0.75 J/g and Tpeak = 226.68 • C), similarly to the observations of Nascimento et al [3]. It was not possible to identify eventual endothermic events related to β-CD mass loss at high temperatures [2,40] because a maximum temperature of 300 • C was set in this study. β-CD complexes obtained by KND and SL showed similar behavior ( Figure 6A) with Tonset = 27.07 • C (∆H = 48.46 J/g and Tpeak = 42.72 • C) and 24.07 • C (∆H = 142.10 J/g and Tpeak = 48.86 • C), respectively, both related to dehydration, but occurring at lower temperatures when compared to β-CD.…”

Section: Differential Scanning Calorimetry (Dsc)mentioning

confidence: 77%

Development and Evaluation of Antimicrobial and Modulatory Activity of Inclusion Complex of Euterpe oleracea Mart Oil and β-Cyclodextrin or HP-β-Cyclodextrin

Magalhães

Macedo

Pacheco

et al. 2020

IJMS

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The development of inclusion complexes is used to encapsulate nonpolar compounds and improve their physicochemical characteristics. This study aims to develop complexes made up of Euterpe oleracea Mart oil (EOO) and β-cyclodextrin (β-CD) or hydroxypropyl-β-cyclodextrin (HP-β-CD) by either kneading (KND) or slurry (SL). Complexes were analyzed by molecular modeling, Fourier-transform infrared spectroscopy, scanning electron microscopy, powder X-ray diffraction, thermogravimetry analysis and differential scanning calorimetry. The antibacterial activity was expressed as Minimum Inhibitory Concentration (MIC), and the antibiotic resistance modulatory activity as subinhibitory concentration (MIC/8) against Escherichia coli, Streptomyces aureus, Pseudomonas aeruginosa and Enterococcus faecalis. Inclusion complexes with β-CD and HP-β-CD were confirmed, and efficiency was proven by an interaction energy between oleic acid and β-CD of −41.28 ± 0.57 kJ/mol. MIC values revealed higher antibacterial activity of complexes compared to the isolated oil. The modulatory response of EOO and EOO-β-CD prepared by KND as well as of EOO-β-CD and EOO-HP-β-CD prepared by SL showed a synergistic effect with ampicillin against E. coli, whereas it was not significant with the other drugs tested, maintaining the biological response of antibiotics. The antimicrobial response exhibited by the complexes is of great significance because it subsidizes studies for the development of new pharmaceutical forms.

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“…Studies show that natural products without antibacterial activity can potentiate the effect of certain antibiotics, which is known as synergism [19]. The C. ceanothifolius essential oil is characterized by the presence of bicyclogermacrene, germacrene D and caryophyllene sesquiterpenes as major compounds (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Essential Oil of Croton ceanothifolius Baill. Potentiates the Effect of Antibiotics against Multiresistant Bacteria

et al. 2020

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This study is a pioneer in reporting the antibacterial properties of the species Croton ceanothifolius Baill. The genus Croton belongs to the family Euphorbiaceae composed of numerous species with documented biological activities. However, the pharmacological properties of C. ceanothifolius remain poorly understood. The leaves of this plant were submitted to hydrodistillation for essential oil (CcEO) extraction and the phytochemical characterization of the oil was performed by GC/MS. The minimum inhibitory concentration of the CcEO was determined for the evaluation of antibacterial activity against multiresistant strains of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The antibiotic-modulating activity of the oil, in combination with antibiotics, was also evaluated. The combination of the CcEO with penicillin, norfloxacin, and gentamicin presented a synergistic effect. This effect was more significant for the association with antibiotics of the quinolone and aminoglycoside classes against Escherichia coli. The association of oil with gentamicin showed better results with regard to the Gram-positive strain. The association of the oil with norfloxacin against P. aeruginosa also showed synergism, but the association with penicillin did not change the effect of this antibiotic. Thus, it is concluded that C. ceanothifolius essential oil selectively potentiates the action of antibiotics against multiresistant strains.

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Antimicrobial, modulatory and chemical analysis of the oil of Croton limae

Cited by 16 publications

References 29 publications

Chemical Constituents and Biological Activities of Croton heliotropiifolius Kunth

Chemical Constituents and Biological Activities of Croton heliotropiifolius Kunth

Development and Evaluation of Antimicrobial and Modulatory Activity of Inclusion Complex of Euterpe oleracea Mart Oil and β-Cyclodextrin or HP-β-Cyclodextrin

Essential Oil of Croton ceanothifolius Baill. Potentiates the Effect of Antibiotics against Multiresistant Bacteria

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