2011
DOI: 10.1128/aac.00206-11
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Antimicrobial Penetration and Efficacy in an In Vitro Oral Biofilm Model

Abstract: The penetration and overall efficacy of six mouthrinse actives was evaluated by using an in vitro flow cell oral biofilm model. The technique involved preloading biofilm cells with a green fluorescent dye that leaked out as the cells were permeabilized by a treatment. The loss of green color, and of biomass, was observed by time-lapse microscopy during 60 min of treatment under continuous flow conditions. The six actives analyzed were ethanol, sodium lauryl sulfate, triclosan, chlorhexidine digluconate (CHX), … Show more

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Cited by 96 publications
(73 citation statements)
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“…Triclosan (purity .97 %, diffusion coefficient in water 6.1 Â 10 À10 m 2 s À1 at 25 8C [17] ), Ludox LS silica nanoparticles (mean radius of 7.5 nm as previously measured, [9,18] diffusion coefficient of 2.7 Â 10 À11 m 2 s…”
Section: Experimental Materialsmentioning
confidence: 69%
“…Triclosan (purity .97 %, diffusion coefficient in water 6.1 Â 10 À10 m 2 s À1 at 25 8C [17] ), Ludox LS silica nanoparticles (mean radius of 7.5 nm as previously measured, [9,18] diffusion coefficient of 2.7 Â 10 À11 m 2 s…”
Section: Experimental Materialsmentioning
confidence: 69%
“…Also, care should be taken when comparing different biofilms, since the laser penetration on biofilms with different depth can also result in misinterpretation of live/dead data. To decipher the mechanisms of biocide tolerance associated with biofilm architecture, procedures compatible with real-time observation of their action on cell viability were developed (Bridier et al, 2011a, b;Corbin et al, 2011;Davison et al, 2010). Davison and his collaborators proposed an indirect labeling procedure to visualize the spatial and temporal patterns of biocide action against biofilms (Davison et al, 2010).…”
Section: Microscopy Methodsmentioning
confidence: 99%
“…For a long time it appeared that molecular weight was a limiting factor for antibiotic penetration into biofilms, however recent studies have demonstrated that the molecular dimensions of antibiotics have no significant relevance [31,32]. Indeed, in an in vitro study by Stewart et al, daptomycin, one of the antibiotics with a higher molecular weight, was confirmed to have freely diffusive penetration into a large, dense cluster of staphylococcal biofilms [33].…”
Section: Biofilmmentioning
confidence: 99%