Antimicrobial peptide based magnetic recognition elements and Au@Ag-GO SERS tags with stable internal standards: a three in one biosensor for isolation, discrimination and killing of multiple bacteria in whole blood

Yuan, Kaisong; Mei, Qingsong; Guo, Xinjie; Xu, Youwei; Yang, Danting; Jurado‐Sánchez, Beatriz; Sheng, Bingbing; Liu, Chusheng; Hu, Ziwei; Gao, Yu; Ma, Hong-Ming; Gao, Hao; Haisch, Christoph; Nießner, Reinhard; Jiang, Zhengjing; Zhou, Haibo

doi:10.1039/c8sc04637a

Cited by 161 publications

(99 citation statements)

References 48 publications

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“…The Rapid detection of micro-organisms responsible for diseases is extremely important for initiating treatment of the concerned disease. In addition to this, detailed analysis of metabolites involved in the biological process is necessary for successful treatment of the disease [39]. Because of this reason, a large amount of attention is being given to rapid, specific and sensitive detection methods.…”

Section: Resultsmentioning

confidence: 99%

Gold and silver nanoparticles used for SERS detection of S. aureus and E. coli

Ankamwar

Gharpure

2020

Nano Ex.

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Surface enhanced Raman scattering (SERS) is emerging as a robust analytical method used in sensing applications in chemical as well as biological systems. SERS has been reported to be used in fast detection of micro-organisms up to the specificity of strain identification. However, use of SERS is tricky because of difficulties involved in selection of SERS active substrate so as to give uniform, sensitive as well as reproducible results. We have synthesized silver and gold nanoparticles using chemical, electrochemical and microwave-assisted methods followed by their characterization. Uses of these nanoparticles in association with micro-organisms such as S. aureus and E. coli have been analyzed using SERS to generate signature spectra. This demonstrates use of so synthesized gold and silver nanoparticles as SERS active substrates for rapid detection of micro-organisms which pave way to find applications in disease diagnostics.

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Section: Resultsmentioning

confidence: 99%

Gold and silver nanoparticles used for SERS detection of S. aureus and E. coli

Ankamwar

Gharpure

2020

Nano Ex.

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“…Thus, various strategies have been developed for precise biosensing and biomarker detecting, such as immunofluorescence, real‐time PCR, etc . Among them, ferromagnetic‐particle‐assisted methods strikingly facilitate this process by broadening the species of biological detection samples, as well as making the detection ultrasensitive and easy‐operated, thus opening up new avenues in efficient, rapid, accurate biodetection and bioanalysis …”

Section: Ferrofluid‐based Detection Diagnosis and Therapymentioning

confidence: 99%

“…Besides, the total yeast and Brettanomyces bruxellensis content in wine were quantified through concanavalin A and anti‐Brett polyclonal antibodies labeled ferromagnetic particles, separately . In addition, as displayed by Yuan et al, the surface of ferromagnetic particles was modified and functionalized by antimicrobial peptide, which could then enrich, capture, and separate targeted bacteria in blood samples . By further combining the captured bacteria with surface‐enhanced Raman scattering tags, the concentration of the bacteria could be read out simply and accurately.…”

Section: Ferrofluid‐based Detection Diagnosis and Therapymentioning

confidence: 99%

Flexible Ferrofluids: Design and Applications

et al. 2019

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Ferrofluids, also known as ferromagnetic particle suspensions, are materials with an excellent magnetic response, which have attracted increasing interest in both industrial production and scientific research areas. Because of their outstanding features, such as rapid magnetic reaction, flexible flowability, as well as tunable optical and thermal properties, ferrofluids have found applications in various fields, including material science, physics, chemistry, biology, medicine, and engineering. Here, a comprehensive, in‐depth insight into the diverse applications of ferrofluids from material fabrication, droplet manipulation, and biomedicine to energy and machinery is provided. Design of ferrofluid‐related devices, recent developments, as well as present challenges and future prospects are also outlined.

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“…Among the four bacteria strains, S. aureus, S. epidermidis, and S. anginosus were Gram-positive bacteria frequently causing bacterial infectious diseases, while E. coli was a common Gram-negative bacterial pathogen exhibiting antibiotic resistance. As for concentrations, although several traditional bacteria detection methods showed a lower detection limit in vitro, [29][30][31] 10 4 CFU mL −1 was still considered sensitive enough for in vivo application. [32] Fluorescence signals could be detected in the bacterial sediments after centrifugation and PBS rinsing, indicating successful labeling of bacteria by PbS QDs (Figure 3a1-d1).…”

Section: Time Window Of Antibiotic Administration Is a Critical But Lmentioning

confidence: 99%

In Vivo Dynamic Monitoring of Bacterial Infection by NIR‐II Fluorescence Imaging

Feng

Chen

Pei

et al. 2020

Small

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Time window of antibiotic administration is a critical but long‐neglected point in the treatment of bacterial infection, as unnecessary prolonged antibiotics are increasingly causing catastrophic drug‐resistance. Here, a second near‐infrared (NIR‐II) fluorescence imaging strategy based on lead sulfide quantum dots (PbS QDs) is presented to dynamically monitor bacterial infection in vivo in a real‐time manner. The prepared PbS QDs not only provide a low detection limit (104 CFU mL−1) of four typical bacteria strains in vitro but also show a particularly high labeling efficiency with Escherichia coli (E. coli). The NIR‐II in vivo imaging results reveal that the number of invading bacteria first decreases after post‐injection, then increases from 1 d to 1 week and drop again over time in infected mouse models. Meanwhile, there is a simultaneous variation of dendritic cells, neutrophils, macrophages, and CD8+ T lymphocytes against bacterial infection at the same time points. Notably, the infected mouse self‐heals eventually without antibiotic treatment, as a robust immune system can successfully prevent further health deterioration. The NIR‐II imaging approach enables real‐time monitoring of bacterial infection in vivo, thus facilitating spatiotemporal deciphering of time window for antibiotic treatment.

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Antimicrobial peptide based magnetic recognition elements and Au@Ag-GO SERS tags with stable internal standards: a three in one biosensor for isolation, discrimination and killing of multiple bacteria in whole blood

Abstract: A SERS based biosensor has been developed for isolation, detection and killing of multiple bacterial pathogens.

Cited by 161 publications

References 48 publications

Gold and silver nanoparticles used for SERS detection of S. aureus and E. coli

Gold and silver nanoparticles used for SERS detection of S. aureus and E. coli

Flexible Ferrofluids: Design and Applications

In Vivo Dynamic Monitoring of Bacterial Infection by NIR‐II Fluorescence Imaging

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