Antimicrobial Peptides: Bringing Solution to the Rising Threats of Antimicrobial Resistance in Livestock

Saeed, Shamsaldeen Ibrahim; Mergani, AhmedElmontaser; Aklilu, Erkihun; Kamaruzzman, Nor Fadhilah

doi:10.3389/fvets.2022.851052

Cited by 24 publications

(9 citation statements)

References 69 publications

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“…With expanding bacterial resistance to conventional antibiotics, antimicrobial peptides have become increasingly important as a last resort for combating multi-resistant bacteria; and some of these have already been used in veterinary medicine (Davis and Janssen, 2020). Antimicrobial peptides have certain advantages, such as adverse effects against biofilm formation and immune response stimulators, thereby enhancing immunity and protection against infections (Saeed et al ., 2022).…”

Section: Resultsmentioning

confidence: 99%

Virulence factors, antimicrobial resistance and phylogeny of bovine mastitis-associatedStreptococcus dysgalactiae

Crippa

Rodrigues

Tomazi

et al. 2023

Journal of Dairy Research

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We carried out a thorough genetic evaluation of Streptococcus dysgalactiae isolated from clinical bovine mastitis cases and performed a phylogenetic analysis to represent the evolutionary relationship between S. dysgalactiae sequences. A total of 35 S. dysgalactiae strains were isolated from cases of clinical mastitis identified at a large commercial dairy farm located near Ithaca, New York. Whole-genome sequencing identified twenty-six antibiotic resistance genes, four of which were acquired genes, in addition to fifty virulence genes. Multi-locus sequence typing detected three new sequence types (STs). We conclude that a high proportion of this microorganism carries multiple virulence determinants and resistance genes, and that this indicates its potential to cause mastitis. Eight different STs were identified, of which ST453 (n = 17) was the most prevalent and ST714, ST715, ST716 were novel STs.

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Section: Resultsmentioning

confidence: 99%

Virulence factors, antimicrobial resistance and phylogeny of bovine mastitis-associatedStreptococcus dysgalactiae

Crippa

Rodrigues

Tomazi

et al. 2023

Journal of Dairy Research

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“…These peptides exhibit potential activity against yeasts, fungi and bacteria. Although several AMPs derived from plants have been identified till date, none of them has been clinically approved yet (Saeed et al 2022 ).…”

Section: Peptides As Therapeuticsmentioning

confidence: 99%

“…Nisin is 34 amino acid peptide sequence that has been commercially approved for the treatment of bovine mastitis (Li et al 2021 ). Another 40 amino acid AMP, plectasin isolated from the fungus Pseudoplectania nigrella has exhibited strong bactericidal activity against multidrug resistant strains of S. aureus (Saeed et al 2022 ).…”

Section: Peptides As Therapeuticsmentioning

confidence: 99%

Strategic Approaches to Improvise Peptide Drugs as Next Generation Therapeutics

Barman

Joshi

Sharma

et al. 2023

Int J Pept Res Ther

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In recent years, the occurrence of a wide variety of drug-resistant diseases has led to an increase in interest in alternate therapies. Peptide-based drugs as an alternate therapy hold researchers’ attention in various therapeutic fields such as neurology, dermatology, oncology, metabolic diseases, etc. Previously, they had been overlooked by pharmaceutical companies due to certain limitations such as proteolytic degradation, poor membrane permeability, low oral bioavailability, shorter half-life, and poor target specificity. Over the last two decades, these limitations have been countered by introducing various modification strategies such as backbone and side-chain modifications, amino acid substitution, etc. which improve their functionality. This has led to a substantial interest of researchers and pharmaceutical companies, moving the next generation of these therapeutics from fundamental research to the market. Various chemical and computational approaches are aiding the production of more stable and long-lasting peptides guiding the formulation of novel and advanced therapeutic agents. However, there is not a single article that talks about various peptide design approaches i.e., in-silico and in-vitro along with their applications and strategies to improve their efficacy. In this review, we try to bring different aspects of peptide-based therapeutics under one article with a clear focus to cover the missing links in the literature. This review draws emphasis on various in-silico approaches and modification-based peptide design strategies. It also highlights the recent progress made in peptide delivery methods important for their enhanced clinical efficacy. The article would provide a bird’s-eye view to researchers aiming to develop peptides with therapeutic applications. Graphical Abstract

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“…By introducing outer membrane-disrupting peptides or polycationic peptides, the engineered endolysin was expanded to the treatment of gram-negative pathogens [ 18 , 19 ]. Antimicrobial peptide (AMP) is found in nature and is part of the innate immune system of different organisms with the membrane-disrupting ability [ 20 ]. Cecropins were originally isolated from the insect Hyalophora cecropia and have antibacterial activity as antimicrobial peptides against gram-negative pathogens [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Bactericidal Effect of Cecropin A Fused Endolysin on Drug-Resistant Gram-Negative Pathogens

Lim

Hong

Jung

et al. 2022

J. Microbiol. Biotechnol.

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The rapid spread of superbugs leads to the escalation of infectious diseases, which threatens public health. Endolysins derived from bacteriophages are spotlighted as promising alternative antibiotics against multi-drug resistant bacteria. In this study, we isolated and characterized the novel Salmonella typhimurium phage PBST08. Bioinformatics analysis of the PBST08 genome revealed putative endolysin ST01 with a lysozyme-like domain. Since the lytic activity of the purified ST01 was minor, probably owing to the outer membrane, which blocks accessibility to peptidoglycan, antimicrobial peptide cecropin A (CecA) was fused to the N-terminus of ST01 to disrupt the outer membrane. The resulting CecA::ST01 has been shown to have increased bactericidal activity against gram-negative pathogens including Pseudomonas aeruginosa , Klebsiella pneumoniae , Acinetobacter baumannii , Escherichia coli , and Enterobacter cloacae and the most affected target was A. baumannii . In the presence of 0.25 μM CecA::ST01, A. baumannii ATCC 17978 strain was completely killed and CCARM 12026 strain was wiped out by 0.5 μM CecA::ST01, which is a clinical isolate of A. baumannii and resistant to multiple drugs including carbapenem. Moreover, the larvae of Galleria mellonella could be rescued up to 58% or 49% by the administration of CecA::ST01 upon infection by A. baumannii 17978 or CCARM 12026 strain. Finally, the antibacterial activity of CecA::ST01 was verified using 31 strains of five gram-negative pathogens by evaluation of minimal inhibitory concentration. Thus, the results indicate that a fusion of antimicrobial peptide to endolysin can enhance antibacterial activity and the spectrum of endolysin where multi-drug resistant gram-negative pathogens can be efficiently controlled.

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Antimicrobial Peptides: Bringing Solution to the Rising Threats of Antimicrobial Resistance in Livestock

Cited by 24 publications

References 69 publications

Virulence factors, antimicrobial resistance and phylogeny of bovine mastitis-associatedStreptococcus dysgalactiae

Virulence factors, antimicrobial resistance and phylogeny of bovine mastitis-associatedStreptococcus dysgalactiae

Strategic Approaches to Improvise Peptide Drugs as Next Generation Therapeutics

Bactericidal Effect of Cecropin A Fused Endolysin on Drug-Resistant Gram-Negative Pathogens

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