Antimicrobial resistance in Enterococcus spp. isolated from laying hens of backyard poultry floks

Bertelloni, Fabrizio; Salvadori, Claudia; Moni, Annalisa; Cerri, Domenico; Mani, Paolo; Ebani, Valentina Virginia

doi:10.5604/12321966.1185771

Cited by 28 publications

(29 citation statements)

References 21 publications

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“…Enterococcus spp. have a strong ability to acquire, express and transfer antimicrobial resistance [ 21 ], and our findings are in agreement with other studies reported in the literature [ 22 ].…”

Section: Resultssupporting

confidence: 93%

Antimicrobial Activity and Composition of Five Rosmarinus (Now Salvia spp. and Varieties) Essential Oils

et al. 2021

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Salvia rosmarinus Spenn. and Salvia jordanii J.B.Walker are aromatic evergreen shrubs belonging to the Lamiaceae family. Their aerial parts have been used since ancient times as natural preservatives. The present study reported the investigation of the chemical profile and the extraction yield of the essential oils (EOs) obtained from the dried aerial parts of four cultivars of Salvia rosmarinus (‘Boule’; ‘Vicomte de Noailles’; ‘Gorizia’; ‘Joyce de Baggio’) and the species S. jordanii, together with their antibacterial and antifungal activities. The phytochemical investigation evidenced a predominance of oxygenated monoterpenes in all the samples (57.5–77.1%), except in ‘Boule’, in which the hydrocarbon form prevailed (50.2%). Principal Component Analysis (PCA) of the matrix taxa × compounds showed that nine compounds have a significant discriminating function between the samples. ‘Vicomte de Noailles’ was characterized by high amounts of camphor and 14-hydroxy-9-epi-(E)-caryophyllene, while ‘Gorizia’ and Jord differed in their predominance of camphene, borneol, bornyl acetate, and α-humulene. Lastly, ‘Boule’ and ‘Joyce de Baggio’ segregated separately and were characterized by high amounts of α-pinene, myrcene, and verbenone. The selected EOs presented a moderate antibacterial activity on the tested bacterial strains and resulted not active on the tested yeast species.

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Section: Resultssupporting

confidence: 93%

Antimicrobial Activity and Composition of Five Rosmarinus (Now Salvia spp. and Varieties) Essential Oils

et al. 2021

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“…Almost half of our isolates (44.6%) showed resistance to tetracycline. Resistance to this antimicrobial has been frequently found in pathogens and commensal bacteria from domestic [16,[63][64][65][66] and wild [58,60,67] animals. Even if β-lactams and tetracycline belong to different antibiotic classes, there could be some shared considerations.…”

Section: Discussionmentioning

confidence: 99%

Pathotypes and Antimicrobial Susceptibility of Escherichia Coli Isolated from Wild Boar (Sus scrofa) in Tuscany

Bertelloni

Cilia

Bogi

et al. 2020

Animals

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Wild boar are among the most widespread wild mammals in Europe. Although this species can act as a reservoir for different pathogens, data about its role as a carrier of pathogenic and antimicrobial-resistant Escherichia coli are still scarce. The aim of this study was to evaluate the occurrence of antimicrobial-resistant and pathogenic Escherichia coli in wild boar in the Tuscany region of Italy. During the hunting season of 2018–2019, E. coli was isolated from 175 of 200 animals and subjected to antimicrobial resistance tests and PCR for detection of resistance and virulence factor genes. The highest resistance rates were against cephalothin (94.3%), amoxicillin–clavulanic acid (87.4%), ampicillin (68.6%), and tetracycline (44.6%). The most detected resistance genes were blaCMY-2 (54.3%), sul1 (38.9%), sul2 (30.9%), and tetG (24.6%). Concerning genes encoding virulence factors, 55 of 175 isolates (31.4%) were negative for all tested genes. The most detected genes were hlyA (47.4%), astA (29.1%), stx2 (24.6%), eaeA (17.1%), and stx1 (11.4%). E. coli was classified as Shiga toxin-producing E. coli (STEC) (21.7%), enterohemorrhagic E. coli (EHEC) (6.3%), enteroaggregative E. coli (EAEC) (5.1%), and atypical enteropathogenic E. coli (aEPEC) (3.4%). Enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), and typical enteropathogenic E. coli (tEPEC) were not detected. Our results show that wild boars could carry pathogenic and antimicrobial-resistant E. coli, representing a possible reservoir of domestic animal and human pathogens.

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“…In this study, high-level resistance to gentamicin among all isolates investigated was not observed, which is in agreement with results obtained by other authors. 57…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Biofilm Formation inEnterococcusspp. Isolated from Humans and Turkeys in Poland

Woźniak-Biel

Bugla-Płoskońska

Burdzy

et al. 2019

Microbial Drug Resistance

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Enterococci are a natural component of the intestinal flora of many organisms, including humans and birds. As opportunistic pathogens, they can cause fatal infections of the urinary tract and endocarditis in humans, whereas in poultry symptoms are joint disease, sepsis, and falls in the first week of life. The study covered 107 Enterococcus strains—56 isolated from humans and 51 from turkeys. Among the isolates investigated Enterococcus faecalis was detected in 80.36% of human and 80.39% of turkey samples. Enterococcus faecium was identified in 8.93% of human and 17.65% of turkey strains. The highest percentage of the strains was resistant to tetracycline as follows: 48 (85.71%) and 48 (94.12%) of human and turkey strains, respectively. Resistance to erythromycin occurred in 37.50% of the human and in 76.47% of turkey strains, otherwise 27.10% of all strains showed resistance to ciprofloxacin. Our study revealed that 25% of human and 15.69% of turkey strains were resistant to vancomycin. Multidrug resistance showed in 32.14% and 43.14% of human and turkey strains, respectively. The tetracycline resistance gene, tetM, was detected in 82.24% of all strains analyzed, whereas the tetO gene was found in 53.57% of human but only in 7.84% of turkey strains. The vancomycin resistance gene ( vanA ) was detected in seven Enterococcus strains (six isolated from turkeys and one from humans). The ermB gene (resistance to macrolide) was detected in 55.14% of all isolates (42.86% of human and 68.63% of turkey strains), whereas the ermA gene was detected in 17.65% of turkey but only in 3.57% of human isolates. All the strains had the ability to form biofilms. A stronger biofilm was formed after 24-hour incubation by strains isolated from turkeys, whereas after 48 hours of incubation all examined strains produced strong biofilm.

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Antimicrobial resistance in Enterococcus spp. isolated from laying hens of backyard poultry floks

Cited by 28 publications

References 21 publications

Antimicrobial Activity and Composition of Five Rosmarinus (Now Salvia spp. and Varieties) Essential Oils

Antimicrobial Activity and Composition of Five Rosmarinus (Now Salvia spp. and Varieties) Essential Oils

Pathotypes and Antimicrobial Susceptibility of Escherichia Coli Isolated from Wild Boar (Sus scrofa) in Tuscany

Antimicrobial Resistance and Biofilm Formation inEnterococcusspp. Isolated from Humans and Turkeys in Poland

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