Antimicrobial Resistance in Some Gram‐Negative Bacteria Isolated from the Bovine Ejaculate

Kilburn, C; Rooks, DJ; McCarthy, AJ; Murray, Richard D.

doi:10.1111/rda.12127

Cited by 13 publications

(7 citation statements)

References 23 publications

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“…Sphingomonas and Pseudomonas, sensitive to gentamicin in bovine semen. Greater scientific understanding of the transfer of antibiotic resistance, especially in pathogenic bacteria, is needed and concerns over the routine use of antibiotics as a substitute for good animal husbandry practices within intensive farming must be addressed (Kilburn et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

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Boar Semen Bacterial Contamination in Italy and Antibiotic Efficacy in a Modified Extender

Bresciani

Cabassi

Morini

et al. 2014

Italian Journal of Animal Science

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The aims of the study were to identify microbial flora in boar semen under field conditions in northern Italy, to investigate antibiotic resistance and sensitivity of isolated bacteria, and to evaluate elimination of bacteria after storage in two types of extenders added with different antibiotics (amikacin vs gentamicin). A total of 60 boars were collected in 13 pig farms. Bacteriological and mycological investigations were performed immediately on raw semen samples, then at 48 and 120 h of storage on semen diluted randomly in a new short-term modified extender (ME-S) or in a commercial one (CRONOS TM ). Bacterial contamination was found in 63% of raw semen samples and different bacterial species were isolated: E.coli, Serratia marcescens, Staphylococcus epidermidis and aureus, Proteus spp., Streptococcus spp. and Pseudomonas aeruginosa. E. coli was the most isolated contaminant (53%); Pseudomonas aeruginosa was found only in one semen sample. The analysis of variance of factors affecting contamination levels was significant for the farm of origin (P<0.05) and not significant for the breed. Antibiotic resistance of these bacteria was assessed using different antibiotics. Significant differences (P<0.05) between observed and expected frequencies of bacterial isolates resistant or not to the antibiotics contained in the extenders were found. At 48 h of storage a reduction of aerobic contamination was found after ME-S dilution by 85.3% and after CRONOS TM by 63.8%. This paper proved the presence of pathogenic bacteria in semen. We thus believe it is highly advisable to perform periodic microbiological screening of boar semen in the swine industry to avoid the use of low sperm quality.

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Section: Resultsmentioning

confidence: 99%

“…The presence of Ureaplasma diversum, Histophilus somni, Chlamydophila abortus, Escherichia coli, and Sthaphylococcus spp., has been reported during reproductive tract infection of the cows and Pseudomonas, are opportunistic microorganisms of the bull's genital tract(Schlafer and Miller, 2007). Recently,Kilburn et al (2013) …”

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confidence: 99%

Boar Semen Bacterial Contamination in Italy and Antibiotic Efficacy in a Modified Extender

Bresciani

Cabassi

Morini

et al. 2014

Italian Journal of Animal Science

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“…has been widely identified in nature, especially in aquatic environments ( Koskinen et al, 2000 ). So far, they have been reported in samples of bovine ejaculated semen ( Kilburn et al, 2013 ), which might be contaminated by environmental sources. In the current study, the samples of endometritis donkey jennies were collected within 4 to 6 days post-insemination, at which time the semen might not be completely cleared out from the uterus of jennies with endometritis.…”

Section: Discussionmentioning

confidence: 99%

Endometrial and vaginal microbiome in donkeys with and without clinical endometritis

Zhu²,

Mi³

et al. 2022

Front. Microbiol.

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Endometrial and vaginal microbiomes are critical in the study of endometritis, which is an important cause of infertility in donkeys. Our objective was to investigate the difference of the endometrial and vaginal microbiomes between healthy donkey jennies (group C) and jennies with endometritis (group E). Endometrial and vaginal swab samples were collected, and the 16 s rRNA gene amplicon high-throughput sequencing technique was applied to identify the microbial composition in the samples. A similar microbial composition pattern was found between endometrial and vaginal samples, which indicated the impact of the vaginal microbiome on the endometrial microbial environment and health. There was a significant difference of endometrial and vaginal swab samples between the two groups. Ruminococcaceae and Lachnospiraceae were significantly more abundant in endometrial and vaginal microbiomes of group E than in group C. Their dominance was consistent with increased anaerobic bacterial taxa in the functional analysis, which might be associated with the pathogenesis of endometritis in donkeys. Sphingomonadaceae, a bacterial family reported in bovine semen, was statistically more abundant in endometrial microbiome of group E than in group C, which might suggest an association between high abundance of Sphingomonadaceae possibly due to uncleared semen and donkey endometritis. Our study revealed the composition of the vaginal and endometrial microbiomes in healthy and endometritis donkeys. These findings will provide more insights into the pathogenesis of donkey endometritis.

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“…Most members of these genera are antibiotic-resistant bacteria and detrimental to sperm quality. For example, bacteria from genus Sphingomonas and Stenotrophomonas were frequently detected in bovine semen exhibited a wide range of antimicrobial resistance phenotypes which further impaired sperm motility and suppressed early embryonic development [ 39 , 40 ]. Species of Enterobacter could degrade antimicrobial agents in swine extenders, damage sperm motility and membrane integrity, and increase sperm agglutination [ 41 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

The Antibacterial and Antioxidant Roles of Buckwheat Honey (BH) in Liquid Preservation of Boar Semen

Lan

Xie

Pan

et al. 2021

BioMed Research International

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In the present study, we hypothesized that buckwheat honey (BH) should be regarded as a potential alternative to antibacterial and antioxidant agent in liquid storage of boar semen. To this end, boar semen was firstly studied for in vitro dose tolerability to BH by measuring sperm progressive motility. The optimum progressive motility of boar spermatozoa was observed in extender with 0.5% and 0.6% BH addition. Afterward, sperm quality parameters, bacterial profile and composition, total antioxidant (T-AOC), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) levels of control, BH supplementation, antibiotics supplementation, and incorporated supplementation were compared during liquid storage period, to further investigate antibacterial and antioxidant properties of BH. The results showed that BH supplementation significantly improved sperm motility, acrosome integrity, plasma membrane integrity, inhibited opportunistic bacterial growth, and altered microbial compositions at the end of preservation. Additionally, T-AOC, SOD, and CAT levels were significantly higher in the BH supplementation group than those in the control and antibiotic supplementation group, whereas MDA level exhibited opposite change pattern. Importantly, BH addition to the extender was able to exert a synergistic effect in combination of antibiotic use. Our findings suggested that the appropriate concentrations (0.5% and 0.6%) of BH were added to the extender could act antibacterial and antioxidant roles in liquid preservation of boar semen.

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Antimicrobial Resistance in Some Gram‐Negative Bacteria Isolated from the Bovine Ejaculate

Cited by 13 publications

References 23 publications

Boar Semen Bacterial Contamination in Italy and Antibiotic Efficacy in a Modified Extender

Boar Semen Bacterial Contamination in Italy and Antibiotic Efficacy in a Modified Extender

Endometrial and vaginal microbiome in donkeys with and without clinical endometritis

The Antibacterial and Antioxidant Roles of Buckwheat Honey (BH) in Liquid Preservation of Boar Semen

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