Antimicrobial resistance of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Tannerella forsythia in periodontitis patients

Medina, C.M. Ardila; Bedoya-García, Jader-Alexander

doi:10.1016/j.jgar.2020.02.024

Cited by 62 publications

(74 citation statements)

References 26 publications

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“…Consistent with other investigators we observed non‐susceptibility of P. gingivalis to DO (0.02%), CIP (8.03%), DA (1.09%) and AZM (1.14%) (van Winkelhoff et al, 2000a, 2000b; Jaramillo et al, 2005; Ardila et al, 2010; Ardila & Bedoya‐Garcia, 2020). However, others reported P. gingivalis sensitivity to AZM (Pajukanta et al, 1994; Jaramillo et al, 2005) but resistance of some periodontopathogens to tetracycline (Collins et al, 2015).…”

Section: Discussionsupporting

confidence: 92%

Prevalence and antibiotic susceptibility trends of periodontal pathogens in the subgingival microbiota of German periodontitis patients: A retrospective surveillance study

Jepsen

Falk

Brune

et al. 2021

J Clinic Periodontology

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Objective This retrospective surveillance study aimed to follow periodontitis‐associated bacterial profiles and to identify time‐dependent changes in antibiotic susceptibility patterns. Materials and Methods From 2008 to 2015, bacterial specimen from deep periodontal pockets were collected from a total of 7804 German adults diagnosed with periodontitis. Presence of selected bacteria was confirmed by anaerobic culture and nucleic acid amplification. Antimicrobial susceptibility of clinical isolates was tested by disc diffusion with antibiotics used for the treatment of periodontitis and oral infections. The prevalences of periodontal pathogens were calculated and temporal evolution of antimicrobial susceptibility towards amoxicillin, amoxicillin/clavulanic acid, metronidazole, doxycycline, clindamycin, azithromycin, ciprofloxacin and ampicillin was analysed with logistic regression. Results The prevalence of patients harbouring bacteria was 95.9% Fusobacterium nucleatum, 88.0% Tannerella forsythia, 76.4% Treponema denticola, 76.5%, Campylobacter rectus, 76.0% Eikenella corrodens, 75.0% Capnocytophaga spp., 68.2% Porphyromonas gingivalis, 57.7% Peptostreptococcus micros, 43.1% Prevotella intermedia, 30.4% Eubacterium nodatum and 21.5% Aggregatibacter actinomycetemcomitans. In 63.5% of patients, one or more isolates were not susceptible to at least one of the antibiotics tested. The data further revealed a trend towards decreasing susceptibility profiles (p < 0.05) with antibiotic non‐susceptibilities in 37% of patients in 2008 and in 70% in 2015. Conclusions The present study confirmed a high prevalence of periodontal pathogens in the subgingival microbiota of German periodontitis patients. The data revealed an incremental increase in isolates displaying resistance to some antibiotics but no relevant change in susceptibility to amoxicillin and metronidazole.

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Section: Discussionsupporting

confidence: 92%

Prevalence and antibiotic susceptibility trends of periodontal pathogens in the subgingival microbiota of German periodontitis patients: A retrospective surveillance study

Jepsen

Falk

Brune

et al. 2021

J Clinic Periodontology

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“…The few available in vitro data on antibiotic resistance suggest a high susceptibility rate of T. forsythia to metronidazole [ 47 , 48 ]. However, in the present study, AMXTMET showed an even stronger depletion of T. forsythia and T. denticola .…”

Section: Discussionmentioning

confidence: 99%

Non-Surgical Periodontal Therapy with Adjunctive Amoxicillin/Metronidazole or Metronidazole When No Aggregatibacter actinomycetemcomitans Is Detected—A Randomized Clinical Trial

2020

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Background: The aim was to compare two different systemic antibiotics regimens adjunctive to non-surgical periodontal therapy when Aggregatibacter actinomycetemcomitans was not detected in the subgingival biofilm. Methods: A total of 58 patients with periodontitis and with no A. actinomycetemcomitans in the subgingival biofilm were treated with full-mouth subgingival instrumentation and either metronidazole (MET; n = 29) or amoxicillin/metronidazole (AMX/MET; n = 29). Probing depth (PD), clinical attachment level (CAL) and bleeding on probing (BOP) were recorded at baseline, as well as after three and six months. Subgingival biofilm and gingival crevicular fluid were collected and analyzed for major periodontopathogens and biomarkers. Results: PD, CAL and BOP improved at 3 and 6 months (each p < 0.001 vs. baseline) with no difference between the groups. Sites with initial PD ≥ 6 mm also improved in both groups after 3 and 6 months (p < 0.001) with a higher reduction of PD in the AMX/MET group (p < 0.05). T. forsythia was lower in the AMX/MET group after 3 months (p < 0.05). MMP-8 and IL-1β were without significant changes and differences between the groups. Conclusion: When A. actinomycetemcomitans was not detected in the subgingival biofilm, the adjunctive systemic use of amoxicillin/metronidazole results in better clinical and microbiological outcomes of non-surgical periodontal therapy when the application of systemic antibiotics is scheduled.

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“…However, this hypothesis was based on culture-based research, which did not go into great detail about the microbiological diversity in the samples [ 99 ]. Modern approaches have furthermore revealed other bacterial pathogens also closely associated with periodontitis such as the bacterial classes Clostridia and Negativicutes , Erysipelotrichia [ 100 ]; the genera Fusobacterium, Prevotella [ 101 ] and Synergistes [ 102 ]; as well as the species Methanobrevibacter oralis, Methanosarcina mazeii, Methanobacterium curvum/congolense [ 99 , 103 , 104 ], Filifactor alocis [ 100 ], and Aggregatibacter actinomycetemcomitans [ 105 ].…”

Section: Modes Of Action Of Salvadora Persica As Potential Adjuncts During Periodontal Therapy and In Periodontitis-asmentioning

confidence: 99%

Salvadora persica: Nature’s Gift for Periodontal Health

Mekhemar

Geib²,

Kumar

et al. 2021

Antioxidants

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Salvadora persica (SP) extract, displays very valuable biotherapeutic capacities such as antimicrobial, antioxidant, antiparasitic and anti-inflammatory effects. Numerous investigations have studied the pharmacologic actions of SP in oral disease therapies but its promising outcomes in periodontal health and treatment are not yet entirely described. The current study has been planned to analyze the reported effects of SP as a support to periodontal therapy to indorse regeneration and healing. In consort with clinical trials, in vitro investigations show the advantageous outcomes of SP adjunctive to periodontal treatment. Yet, comprehensive supplementary preclinical and clinical investigations at molecular and cellular levels are indispensable to reveal the exact therapeutic mechanisms of SP and its elements for periodontal health and therapy.

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Antimicrobial resistance of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Tannerella forsythia in periodontitis patients

Cited by 62 publications

References 26 publications

Prevalence and antibiotic susceptibility trends of periodontal pathogens in the subgingival microbiota of German periodontitis patients: A retrospective surveillance study

Prevalence and antibiotic susceptibility trends of periodontal pathogens in the subgingival microbiota of German periodontitis patients: A retrospective surveillance study

Non-Surgical Periodontal Therapy with Adjunctive Amoxicillin/Metronidazole or Metronidazole When No Aggregatibacter actinomycetemcomitans Is Detected—A Randomized Clinical Trial

Salvadora persica: Nature’s Gift for Periodontal Health

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